[1]温强,叶金山,于宏,等.兔耳风属3个近缘种ISSR分子鉴别[J].南京林业大学学报(自然科学版),2008,32(03):139-141.[doi:10.3969/j.jssn.1000-2006.2008.03.033]
 WEN Qiang,YE Jin-shan,YU Hong,et al.ISSR molecular identification for three species in Ainsliaea[J].Journal of Nanjing Forestry University(Natural Science Edition),2008,32(03):139-141.[doi:10.3969/j.jssn.1000-2006.2008.03.033]
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兔耳风属3个近缘种ISSR分子鉴别
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
32
期数:
2008年03期
页码:
139-141
栏目:
研究简报
出版日期:
2008-05-20

文章信息/Info

Title:
ISSR molecular identification for three species in Ainsliaea
文章编号:
1000-2006(2008)03-0139-03
作者:
温强叶金山于宏江香梅
江西省林业科学研究院, 江西省植物生物技术重点实验室, 江西 南昌 330032
Author(s):
WEN Qiang YE Jin-shan YU Hong JIANG Xiang-mei
Jiangxi Academy of Forestry Sciences, Jiangxi Provincial Bio-tech Key Leg for Plant, Nanchang 330032, China
关键词:
杏香兔耳风 红背兔耳风 铁灯兔耳风 ISSR分子鉴别
Keywords:
Ainsliaea fragrans A. rubrifolia A. macroclinidioides iISSR molecular identification
分类号:
S338; Q943
DOI:
10.3969/j.jssn.1000-2006.2008.03.033
文献标志码:
A
摘要:
<正>以稻草为原料,利用自制的炭化设备制备稻草炭和稻草醋液,研究炭化温度对稻草炭、稻草醋液以及稻草燃气得率的影响,采用元素分析仪、电感耦合等离子直读光谱仪对稻草炭的元素组成进行了测试与分析。结果表明:稻草炭和稻草燃气的得率随炭化终点温度升高而下降,稻草醋液的得率则随炭化终点温度的升高而升高。稻草炭富含作物生长必需的氮、磷、钾等多种营养元素,其中碳、氮、磷、钾含量分别为3.70×104、8.18×104、3.96×103、3.10×104mg/kg。研究了稻草炭添加量对小白菜生长量的影响,结果表明:当稻草炭的添加量占土壤总量的8%时,小白菜生长量提高较大,增幅为20.87%。
Abstract:
Three difficultly-divisional species, i. e., Ainsliaea fragrans, A. macroclinidioides and A. rubrifolia were studied using the ISSR molecular identification system. Twenty primers were screened out from 100 ISSR UBC. They could generate reproducible polymorphic fragments. The particular bands were expanded to make the ISSR fingerprint by primer UBC807, UBC826, UBC834, UBC843, UBC853, and UBC895. It could be used to discriminate three species. Furthermore, according to the alike coefficient UPGMA showed that the re]ative relation of A. fragrans and A. rubrifolia was closer than that of A. fragrans and A. macroclinidioide. The research could be responded for the conservancy and development of the chinese herbal medicine resonrce, and providing a technical support of building up the stable qnality evaluation system of chinese herbal medicine.
更新日期/Last Update: 2013-05-20