[1]李德伟,John C Sutton.拮抗微生物防治仙客来灰霉菌Botrytis cinerea的评估[J].南京林业大学学报(自然科学版),2009,33(01):000.[doi:10.3969/j.jssn.1000-2006.2009.01.001]
 LI De wei,John C Sutton.Evaluation of antagonistic microorganisms for biocontrol of Botrytis cinerea in cyclamen[J].Journal of Nanjing Forestry University(Natural Science Edition),2009,33(01):000.[doi:10.3969/j.jssn.1000-2006.2009.01.001]

拮抗微生物防治仙客来灰霉菌Botrytis cinerea的评估




Evaluation of antagonistic microorganisms for biocontrol of Botrytis cinerea in cyclamen
李德伟John C Sutton
加拿大圭尔夫大学环境生物系,Guelph,ON,N1G 2W1,Canada
LI DeweiJohn C Sutton
Department of Environmental Biology,University of Guelph,Guelph,ON,N1G 2W1,Canada
拮抗微生物生物防治Botrytis cinereaClonostachys rosea仙客来Trichoderma koningii
antagonistic microorganismsbiocontrolBotrytis cinereaClonostachys roseaCyclamenTrichoderma koningii
以仙客来叶圆片方法对灰霉菌Botrytis cinerea的生防效果进行了评估,结果发现Clonostachys rosea和Trichoderma koningii抑制B. cinerea的效果达94%~100%,其效果优于所测试的从仙客来上分离的真菌和细菌的多数菌株。用整株仙客来测试生防菌效果表明,C.rosea在叶片、花瓣、叶柄和花梗上抑制B.cinerea效果分别达96%、58%、53%、77%;而Trichoderma koningii的效果分别达100%、42%、100%、 90%。 以析因试验设计对B.cinerea和C.rosea或T.koningii施用浓度在生防效果上的交互效应进行了测定,结果表明:当C.rosea的接种浓度是病原菌浓度的10倍或10倍以上时,其在叶片、花瓣、叶柄和花梗上抑制B.cinerea的效果分别达73%~100%、52%~86%、100%、54%~68%;当T.koningii的接种浓度为106个/mL或以上时,其抑制B.cinerea在仙客来(接种浓度为103~106个/mL时)各部分发生的效果分别为:叶片100%、叶柄62%~100%、花瓣38%~96%、花梗 56%~100%。C.rosea在叶龄为2、3、5、6 周的叶片上的生防效果更好,而在叶龄为4 周的叶片上效果差些,在叶龄为1 周的叶片上无效果。T.koningii在叶龄为1~6 周的叶片上均可有效地抑制B.cinerea的发生,抑制效果达89%~100%。C.rosea和T.koningii均是在温室内防治仙客来灰霉菌B.cinerea的具潜力的有效生防菌,但T.koningii的防治效果显著地优于C.rosea的效果。
In biocontrol essays on cyclamen leaf disks,Clonostachys rosea and Trichoderma koningii suppressed Botrytis cinerea by 94%—100%,and were more effective than most fungi and bacteria isolated from cyclamen.Biocontrol effectiveness on whole plants showed that C.rosea suppressed B.cinerea in leaves,petals,petiole,and pedicels by 96%,58%,53%,and 77%,respectively and T.koningii by 100%,42%,100% and 90%,respectively.The effects of inoculum concentrations of B.cinerea interacting with C.rosea or T.koningii respectively on biocontrol were examined in factorial design experiments.C.rosea suppressed B.cinerea in lamina,petals,petiole,and pedicels by 73%—100%,52%—86%,100% and 54%—68%,respectively,when inoculum concentration of the antagonist was 10 times more than that of the pathogen.When inoculum concentration of T.koningii was at or over 106 conidia/mL,it suppressed B.cinerea at concentrations of 103—106conidia/mL in lamina by 100%,in petioles by 62%—100%,in petal by 38%—96% and in pedicels by 56%—100%.C.rosea was more effectively on 2,3,5 and 6 weekold leaves,but less effective on 4 weekold leaves and no effect on 1 weekold leaves.T.koningii suppressed B.cinerea on 1 to 6 weekold leaves very effectively ranging from 89% to 100%.We concluded that C.rosea and T.koningii were potentially effective biocontrol agents against B.cinerea in cyclamen in greenhouses,but the effectiveness of T.koningii was significantly better than that of C.rosea.


[1]Grey Wilson C.Cyclamen: A Guide for Gardeners,Horticulturists and Botanists[M].Portland,Oregon:Timber Press,1997.
[2]Widmer E R.Cyclamen Larson R A .Introduction to Floriculture[M]. 2nd edition. San Diego: Academic Press,1992.
[3]Saunders D E.Cyclamen,a Gardener’s Guide to the Genus[M].UK,Pershore an Alpine Garden Society Publication,1973.
[4]Tompkins C M,Hansen H N.Cyclamen petal spot,caused by Botrytis cinerea,and its control[J].Phytopathology,1948,38: 114117.
[5]Fokkema N J.Biological control of fungal plant diseases[J].Entomophaga,1996,41: 333342.
[6]Troliner J C.Epidemiology of Botrytis blight of greenhouse floral crops[D].Raleigh: North Carolina State University,1983.
[7]ColeySmith J R,Verhoeff K, Jarvis W R.The Biology of Botrytis[M].London: Academic Press,1980.
[8]Dubos B.Biological control of Botrytis: stateoftheart.in recent advance in Botrytis research[C]// Proceedings of the 10th International Botrytis Symposium. Heraklion,Crete,Greece,1992.
[9]Elad Y.Responses of plants to infection by Botrytis cinerea and novel means involved in reducing their susceptibility to infection[J].Biol Rev,1997,72: 381422.
[10]Jarvis W R.Botryotinia and Botrytis Species: taxonomy physiology and pathogenicity[R].Ottawa: Canada Department of Agriculture,1977.
[11]Elad Y,Evensen K.Physiological aspects of resistance to Botrytis cinerea[J].Phytopathology,1995,85: 637643.
[12]El-Ghaouth A,Wilson C L,Wisniewski M.Ultrastructural and cytochemical aspects of the biological control of Botrytis cinerea by Candida saitoana in apply fruit[J].Phytopathology,1998,88: 282291.

[13]Bollen G J,Scholten G.Acquired resistance to benomyl and some other systemic fungicides in a strain of Botrytis cinerea in cyclamen[J].Neth J Plant Pathol,1971,77: 8390.
[14]Whipps J M,McQuilken M P.Aspects of biocontrol of fungal plant pathogens[C]// Jones D G.Exploitation of Microorganisms. London: Chapman & Hall,1993:4579.
[15]Reglinski T,Lyon G D,Newton A C.The control of Botrytis cinerea and Rhizoctonia on lettuce using elicitors extracted from yeast cell walls[J].J Plant Dis Prot,1995,102: 257266.
[16]Ghini R.Occurrence of resistance of fungicides in Botrytis cinerea strains in the state of Sao Paulo[J].Fitopatologia Brasileira,1996,21: 285288.
[17]Gulino M L.Control of Botrytis rot of grapes and vegetables with Trichoderma spp.[C]// Tjamos E C,Papavizas G C, Cook R J.Biological Control of Plant Diseases Progress and Challenges for the Future.New York: Plenum,1992.
[18]Likhachev A N,Sale M.Pathogenicity of the original and fungicideresistance strains of Botrytis cinerea Pers.:Fr [J].Mikologiya I Fitopatologiya,1991,25: 240243.
[19]Pappas A C.Inadequate control of grey mould on cyclamen by dicarboximide fungicides in Greece[J].Pflanzneschutz (J Pl Dis Prot),1982,89: 5258.
[20]van Dommelen L,Bollen J G.Antagonism between benomylresistant fungi on cyclamen sprayed with benomyl[J].Acta Bot Neerl,1973,22: 169170.
[21]Elad Y,Yunis H,Katan T.Multiple fungicide resistance to benzimidazoles,dicarboximides and diethofencarb in field isolates of Botrytis cinerea in Israel[J].Plant Pathol,1992,41: 4146.
[22]Raposo R,Delcan J,Gomez V,Melgarejo P.Distribution and fitness of isolates of Botrytis cinerea with multiple fungicide resistance in Spanish greenhouses[J].Plant Pathol,1996,45: 497505.
[23]Latorre B A.Dicarboximideresistance isolates of Botrytis cinerea from table grape in Chile: Survey and characterization[J].Plant Dis,1994,78: 990994.
[24]Pappas A C.Seasonal fluctuation in benzimidazoles and dicarboximideresistant population of Botrytis cinerea in greenhouse crops[R].ISPP Chemical Newsletter, 1986,7:1011.
[25]De Waard M A,Georgopoulos S G,Hollomon D W,et al.Chemical control of plant diseases: Problems and prospects[J].Ann Rev Phytopathol,1993,31: 403421.
[26]Rhodes D J.Formulation of biological control agents[C]// Jones D G.Exploitation of Microorganisms.London: Chapman & Hall,1993.
[27]Harman G E,Latorre B,Agosin E,San Martin R,Riefel D G,Nielsen P A,Tronsmo A,Pearson R C.Biological and integrated control of Botrytis bunch rot of grape using Trichoderma spp.[J].Biological Control,1996,7: 259266.
[28]Kamensky M,Ovadis M,Chet I,Chernin L..Soilborne strain IC14 of Serratia plymuthica with multiple mechanisms of antifungal activity provides biocontrol of Botrytis cinerea and Sclerotinia sclerotiorum diseases[J].Soil Biol & Biochemistry,2003,35: 323331.
[29]Elad Y,Zimand G,Zaqs Y,Zuriel S.Use of Trichoderma harzianum in combination or alternation with fungicides to control cucumber grey mould (Botrytis cinerea) under commercial greenhouse conditions[J].Pl Pathol,1993,42: 324332.
[30]Schena L,Nigro F,Pentimone I,Ligoric A,Ippolito A.Control of postharvest rots of sweet cherries and table grapes with endophytic isolates of Aureobasidium pullulans[J].Postharvest Biol & Tech,2003,30: 209211.
[31]Paul B.A new species of Pythium isolated from burgundian vineyards and its antagonism towards Botrytis cinerea,the causative agent of the grey mould disease[J].Fems Microbiology Letters,2004,234: 269274.
[32]Saligkarias I D,Gravanis F T,Epton H A S.Biological control of Botrytis cinerea on tomato plants by the use of epiphytic yeasts Candida guilliermondii strains 101 and US 7 and Candida oleophila strain I-182: I.in vivo studies [J].Biological Control,2002,25: 143150.
[33]Spadaro D,Vola R,Piano S,Gullino M L.Mechanisms of action and efficacy of four isolates of the yeast Metschnikowia pulcherrima active against postharvest pathogens on apples[J].Postharvest Biol & Technol,2002,24: 123133.
[34]Pennock-Vos M G,Roebroeck E J A,Skrzypczak C.Preliminary results on biological control of Botrytis cinerea in forced tulips[J].Acta Hortic.Wageningen: International Society for Horticultural Science,1990,266: 425428.
[35]Pratella G C,Mari M.Effectiveness of Trichoderma,Gliocladium and Paecilomyces in postharvest fruit protection [J].Postharvest Biol and Technol,1993,3: 4956.
[36]Sutton J C,Peng G.Biocontrol of Botrytis cinerea in strawberry leaves[J].Phytopathology,1993,83: 615621.
[37]Yu Hai,Sutton J C.Effects of inoculum density,wetness duration,and temperature on control of Botrytis cinerea by Gliocladium roseum in raspberry[J].Can J Plant Pathol,1998,20: 243252.
[38]Yu Hai,Sutton J C.Density dynamics of Gliocladium roseum in relation to biological control of Botrytis cinerea in red raspberry[J].Can J Plant Pathol,1999,21: 2332.
[39]Zhang P G,Sutton J C,Hopkin A A.Evaluation of microorganisms for biocontrol of Botrytis cinerea in containergrown black spruce seedlings[J].Can J For Res,1994,24: 13121316.
[40]Sutton J C,Liu W,Huang R,OwenGoing N.Ability of Clonostachys rosea to establish and suppress sporulation potential of Botrytis cinerea in deleafed stems of hydroponic greenhouse tomatoes[J].Biocontrol Sci Technol,2002,12: 413425.
[41]Morandi M A B,Maffia L A,Mizubuti E S G,Alfenas A C.Suppression of Botrytis cinerea sporulation by Clonostachys rosea on rose debris: a valuable component in Botrytis blight management in commercial greenhouses[J].Biological Control,2003,26: 311316.
[42]Capieau K,Stenlid J,Stenstrm E.Potential for biological control of Botrytis cinerea in Pinus sylvestris seedlings[J ].Scand J For Res,2004,19: 312319.
[43]Yohalem D S.Evaluation of fungal antagonists for grey mould management in early growth of pot roses[J].Ann Appl Biol,2004,144: 915.
[44]McCain A H,Sciaroni R H,Welch K E.Gray mold control of cyclamen using Trichoderma harzianum and reduced rates of vinclozolin[J].California Pl Pathol,1983,63: 12.
[45]Someya N,Nakajima M,Hirayae K,Hibi T,Akutsu K.Synergistic antifungal activity of chitinolytic enzymes and prodigiosin produced by biocontrol bacterium,Serratia marcescens strain B2 against gray mold pathogen,Botrytis cinerea[J].J General Pl Pathol,2001,67: 312317.
[46]Khl J,Gerlagh M,De Haas B H Krijger M C.Biological control of Botrytis cinerea in cyclamen with Ulocladium atrum and Gliocladium roseum under commercial growing conditions[J].Phytopathology,1998,88: 568575. [47]Khl J,Lombaersvan der Plas C H,Molhoek W M L,et al.Competitive ability of the antagonists Ulocladium atrum and Gliocladium roseum at temperatures favourable for Botrytis spp.[J].Development Biocontrol (Dordrecht),1999,44: 329346. [48]Khl J,Gerlagh M,Grit G.Biocontrol of Botrytis cinerea by Ulocladium atrum in different production systems of cyclamen [J].Plant Dis,2000,84: 569573. [49]Kessel G J T,De Haas B H,Lombaersvan der Plas C H,et al.Comparative analysis of the role of substrate specificity in biological control of Botrytis elliptica in lily and B.cinerea in cyclamen with Ulocladium atrum[J].European J Pl Pathol,2001,107: 273284. [50]Kessel G J T,De Haas B H,van der Werf W,Kohl J.Competitive substrate colonisation by Botrytis cinerea and Ulocladium atrum in relation to biological control of B.cinerea in cyclamen[J].Mycol Res,2002,106: 716728. [51]Peng G,Sutton J C.Evaluation of microorganisms for biocontrol of Botrytis cinerea in strawberry[J].Can J Plant Pathl,1991,13: 247257. [52]Jarvis W R,Shaw L A,Traquair J A.Factors affecting antagonism of cucumber powdery mildew by Stephanoascus flocculosus and S.rugulosus[J].Mycol Res,1989,92: 162165. [53]Zhou T,Reeleder R D.Application of Epicoccum purpurascens spores to control white mold of snap bean[J].Plant Dis,1989,73: 639642. [54]Sutton J C,Li D-W,Peng G,ValdebenitoSanhueza R M.Gliocladium roseum: a versatile adversary of Botrytis cinerea in crops[J].Plant Dis,1997,81: 316328. [55]Samuels G. Trichoderma: a review of biology and systematics of the genus[J].Mycol Res,1996,100: 923935. [56]Tronsmo A,Hjeljord L G.Biological control with Trichoderma species[C]// Boland G J,Kuykendall L D.PlantMicrobe Interactions and Biological Control.New York: Marcel Dekker,Inc,1998. [57]Eden M A,Hill R A,Stewart A.Biological control of Botrytis stem infection of greenhouse tomatoes[J].Plant Pathol,1996,45: 276284. [58]Rifai M A.A revision of the genus Trichoderma[J].Mycol Papers,1969,116: 1116. [59]Dumas M T,Strunz G M,Boyonoski N W,et al.In vitro interactions between Cylindrocladium floridanum and species of Trichoderma[J].Can J Plant Pathol,1996,18: 325329. [60]Trutmann P,Keane P J.Trichoderma koningii as a biological control agent for Sclerotinia sclerotiorum in southern Australia[J].Soil Biol Biochem,1990,22: 4350. [61]Hadar Y,Harman G E,Taylor A G.Evaluation of Trichoderma koningii and T.harzianum from New York soils for biological control of seed rot caused by Pythium spp.[J].Phytopathology,1984,74: 106110. [62]Domsch K H,Gams W,Anderson T-H.Compendium of Soil Fungi[M].London: Academic Press,1980. [63]Vercesi A,Locci R,Prosser J I.Growth kinetics of Botrytis cinerea on organic acids and sugars in relation to colonization of grape berries[J].Mycol Res,1997,101: 139142. [64]Campos G,Montealegre J R,Perez L M.Changes in soluble reducing sugars and pH in raspberry blossoms,flowers and fruits,at different phenological stages: relationship to infection by Botrytis cinerea Pers[J].Fitopatologia,1995,30: 143147. [65]Kerssies A,Frinking H D.Relations between glasshouse climate and dry weight of petals,epicuticular wax,cuticle,pre harvest flowering period and susceptibility to Botrytis cinerea of gerbera and rose flowers[J].European J Plant Pathol,1996,102: 257263. [66]Li D-W,Sutton J C,Peng G.Gliocladium roseum effectively suppresses Botrytis cinerea in greenhousegrown begonia [J].Phytopathology,1995,85: 1191. [67]Yu H.Relationships of epidemiologic factors,Gliocladium roseum,and bee vectors to gray mold of raspberry caused by Botrytis cinerea[D].Guelph: University of Guelph,Canada,1996. [68]Zhang P G,J Sutton J C,Hopkin A A.Inoculum concentration and time of application of Gliocladium roseum in relation to biocontrol of Botrytis cinerea in black spruce seedlings[J].Can J For Res,1996,26: 360367. [69]Wurms K V,Long P G,Sharrock K R.The potential for resistance to Botrytis cinerea by kiwifruit[J].Crop Protection,1999,18: 427436. [70]Schlosser E.Development stages of leaves from Cyclamen persicum — colonization by Botrytis cinerea Z.Pflanzenkr.Pflanzneschutz[J].J Plant Dis Prot,1978,85: 179185. [71]Hjeljord L,Tronsmo A.Trichoderma and Gliocladium in biological control: an overview[C]// Harman G E,Kubicek C.Trichoderma and Gliocladium: enzymes,biological control and commercial applications.Vol.2.London: Taylor and Francis Ltd,1998.


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收稿日期:2007-09-31修回日期:2008-06-15 基金项目:DMAFRA and Agriculture and AgriFood Canada 作者简介:李德伟(1956—),博士,研究方向为气载真菌与真菌分类。现通讯地址为:Research Mycologist,The Connecticut Agricultural Experiment Station,Valley Laboratory,153 Cook Hill Rd.,Windsor,CTO6095。Email:dewei.li@po.state.ct.us 引文格式:李德伟,John C Sutton.拮抗微生物防治仙客来灰霉菌Botrytis cinerea的评估[J].南京林业大学学报:自然科学版,2009,33 (1):110.
更新日期/Last Update: 2009-05-06