[1]李德伟,John C Sutton.拮抗微生物防治仙客来灰霉菌Botrytis cinerea的评估[J].南京林业大学学报(自然科学版),2009,33(01):000.[doi:10.3969/j.jssn.1000-2006.2009.01.001]
 LI De wei,John C Sutton.Evaluation of antagonistic microorganisms for biocontrol of Botrytis cinerea in cyclamen[J].Journal of Nanjing Forestry University(Natural Science Edition),2009,33(01):000.[doi:10.3969/j.jssn.1000-2006.2009.01.001]
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拮抗微生物防治仙客来灰霉菌Botrytis cinerea的评估
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
33
期数:
2009年01期
页码:
000
栏目:
特稿
出版日期:
2009-01-30

文章信息/Info

Title:
Evaluation of antagonistic microorganisms for biocontrol of Botrytis cinerea in cyclamen
作者:
李德伟John C Sutton
加拿大圭尔夫大学环境生物系,Guelph,ON,N1G 2W1,Canada
Author(s):
LI DeweiJohn C Sutton
Department of Environmental Biology,University of Guelph,Guelph,ON,N1G 2W1,Canada
关键词:
拮抗微生物生物防治Botrytis cinereaClonostachys rosea仙客来Trichoderma koningii
Keywords:
antagonistic microorganismsbiocontrolBotrytis cinereaClonostachys roseaCyclamenTrichoderma koningii
分类号:
S763
DOI:
10.3969/j.jssn.1000-2006.2009.01.001
文献标志码:
A
摘要:
以仙客来叶圆片方法对灰霉菌Botrytis cinerea的生防效果进行了评估,结果发现Clonostachys rosea和Trichoderma koningii抑制B. cinerea的效果达94%~100%,其效果优于所测试的从仙客来上分离的真菌和细菌的多数菌株。用整株仙客来测试生防菌效果表明,C.rosea在叶片、花瓣、叶柄和花梗上抑制B.cinerea效果分别达96%、58%、53%、77%;而Trichoderma koningii的效果分别达100%、42%、100%、 90%。 以析因试验设计对B.cinerea和C.rosea或T.koningii施用浓度在生防效果上的交互效应进行了测定,结果表明:当C.rosea的接种浓度是病原菌浓度的10倍或10倍以上时,其在叶片、花瓣、叶柄和花梗上抑制B.cinerea的效果分别达73%~100%、52%~86%、100%、54%~68%;当T.koningii的接种浓度为106个/mL或以上时,其抑制B.cinerea在仙客来(接种浓度为103~106个/mL时)各部分发生的效果分别为:叶片100%、叶柄62%~100%、花瓣38%~96%、花梗 56%~100%。C.rosea在叶龄为2、3、5、6 周的叶片上的生防效果更好,而在叶龄为4 周的叶片上效果差些,在叶龄为1 周的叶片上无效果。T.koningii在叶龄为1~6 周的叶片上均可有效地抑制B.cinerea的发生,抑制效果达89%~100%。C.rosea和T.koningii均是在温室内防治仙客来灰霉菌B.cinerea的具潜力的有效生防菌,但T.koningii的防治效果显著地优于C.rosea的效果。
Abstract:
In biocontrol essays on cyclamen leaf disks,Clonostachys rosea and Trichoderma koningii suppressed Botrytis cinerea by 94%—100%,and were more effective than most fungi and bacteria isolated from cyclamen.Biocontrol effectiveness on whole plants showed that C.rosea suppressed B.cinerea in leaves,petals,petiole,and pedicels by 96%,58%,53%,and 77%,respectively and T.koningii by 100%,42%,100% and 90%,respectively.The effects of inoculum concentrations of B.cinerea interacting with C.rosea or T.koningii respectively on biocontrol were examined in factorial design experiments.C.rosea suppressed B.cinerea in lamina,petals,petiole,and pedicels by 73%—100%,52%—86%,100% and 54%—68%,respectively,when inoculum concentration of the antagonist was 10 times more than that of the pathogen.When inoculum concentration of T.koningii was at or over 106 conidia/mL,it suppressed B.cinerea at concentrations of 103—106conidia/mL in lamina by 100%,in petioles by 62%—100%,in petal by 38%—96% and in pedicels by 56%—100%.C.rosea was more effectively on 2,3,5 and 6 weekold leaves,but less effective on 4 weekold leaves and no effect on 1 weekold leaves.T.koningii suppressed B.cinerea on 1 to 6 weekold leaves very effectively ranging from 89% to 100%.We concluded that C.rosea and T.koningii were potentially effective biocontrol agents against B.cinerea in cyclamen in greenhouses,but the effectiveness of T.koningii was significantly better than that of C.rosea.

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备注/Memo

备注/Memo:
收稿日期:2007-09-31修回日期:2008-06-15 基金项目:DMAFRA and Agriculture and AgriFood Canada 作者简介:李德伟(1956—),博士,研究方向为气载真菌与真菌分类。现通讯地址为:Research Mycologist,The Connecticut Agricultural Experiment Station,Valley Laboratory,153 Cook Hill Rd.,Windsor,CTO6095。Email:dewei.li@po.state.ct.us 引文格式:李德伟,John C Sutton.拮抗微生物防治仙客来灰霉菌Botrytis cinerea的评估[J].南京林业大学学报:自然科学版,2009,33 (1):110.
更新日期/Last Update: 2009-05-06