[1]朱丽梅,吴小芹*,徐旭凌.细菌JKJS3对松树线虫的杀线活性及菌种的分子鉴定[J].南京林业大学学报(自然科学版),2009,33(06):049.[doi:10.3969/j.jssn.1000-2006.2009.06.011]
　ZHU Li mei,WU Xiao qin*,XU Xu ling.Nematicidal activities of the bacterium strain JKJS3 on plant nematodes isolated from pine trees and its molecular identification[J].Journal of Nanjing Forestry University(Natural Science Edition),2009,33(06):049.[doi:10.3969/j.jssn.1000-2006.2009.06.011]
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细菌JKJS3对松树线虫的杀线活性及菌种的分子鉴定
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
33
期数:
2009年06期
页码:
049
栏目:
研究论文
出版日期:
2009-11-30

文章信息/Info

Title:
Nematicidal activities of the bacterium strain JKJS3 on plant nematodes isolated from pine trees and its molecular identification
作者:
朱丽梅1; 2; 吴小芹1*; 徐旭凌1
1.南京林业大学，江苏省有害生物入侵预防与控制重点实验室，江苏南京210037；2.金陵科技学院，江苏南京210036
Author(s):
ZHU Limei1; 2;  WU Xiaoqin1*;  XU Xuling1
1.Jiangsu Key Laboratory for Prevention and Management of Invasive Specie, Nanjing Forestry University, Nanjing 210037, China; 2.Jinling Institute of Technology, Nanjing 210036, China
关键词:
解淀粉芽孢杆菌; 杀线活性; 分子鉴定
Keywords:
Bacillus amyloliquefaciens;  nematicidal activity;  molecular identification
分类号:
S763
DOI:
10.3969/j.jssn.1000-2006.2009.06.011
文献标志码:
A
摘要:
分离自马尾松的细菌JKJS3是对松材线虫(Bursaphelenchus xylophilus)具有较高杀线活性的拮抗细菌，为了解该菌株对松树体内其他植物线虫的杀线活性并验证经形态学和Biolog方法得到的菌种鉴定结果，笔者测定了该细菌培养滤液对大核滑刃线虫(Aphelenchoides macronucleatus)等7种松树体内植物线虫的杀线活性，并对该菌株的16SrDNA基因进行了扩增测序和比对分析。结果表明：该菌株培养液原滤液对测试线虫24h的杀线率均达到了100%，说明该细菌的杀线谱较广，但培养滤液的不同倍数稀释液对不同种线虫的杀线活性有显著差异，10倍稀释液对霍夫曼尼伞滑刃线虫(B.hofmanni)的杀线活性最高，杀死率为96.9%；其次对李氏长尾线虫(Seinura lii)，杀死率为73.7%；再次对松材线虫和拟松材线虫(B.mucronatus)，杀死率分别为48.7%和39.0%。而对大核滑刃线虫、吴氏长尾线虫(S.wuae)和外滑刃科线虫(Ektaphelenchidae sp.)的杀死率较低，分别为15%、1.5%和0；该菌株的16SrDNA基因序列BLAST比对结果表明，该菌株与菌株Bacillus amyloliquefaciens FZB42最为接近，相似性为99.86%，处于系统发育树的同一分支，进一步验证了其形态学和Biolog细菌鉴定仪的鉴定结果，确定该菌株的分类地位为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。
Abstract:
The bacterium strain JKJS3 isolated from the leaves of Pinus massoniana has been screened for its high nematicidal activity on Bursaphelenchus xylophilus．The nematicidal activities of JKJS3 on 7 plant nematodes isolated from different dead pine trees and its molecular identification have been studied in this paper. The bioassay result obtained from 24 hours treatment of JKJS3 culture filtrate on different nematodes indicated that nematicidal activities not only effected on B.xylophilus, but also on other kinds of nematodes, the mortality of seven kinds of nematodes all attained 100%. The nematicidal activity of the ten times dilution of culture filtrate on B.hofmanni was the highest with mortality of 96.9%, the second was Seinura lii with 73.7%．The mortality of B.xylophilus and B.mucronatus treated with ten times dilution of culture filtrate was 48.7% and 39.0% respectively, however, the mortality of Aphelenchoides macronucleatus, S.wuae and Ektaphelenchidae sp. were rather lower with 1.5%, 1.5% and 0 respectively. The result from 16SrDNA analysis of strain JKJS3 indicated that the strain and Bacillus amyloliquefaciens FZB42 was at the same brand in the phylogenetic tree, their similarity was 99.86%, and it confirmed the former identification by morphological characters and Biolog, which demonstrated the strain JKJS3 belongs to Bacillus amyloliquefaciens.

参考文献/References:

[1]Kamchew Yin, Yusheng Fang, Tarjan A C. A key to species in the genus Bursaphelenchus with a description of Bursaphelenchus hunanensis sp.n.(Nematoda: Aphelenchoididae) found in pinewood in Hunan province, China[J]. Proceedings of the Helminthological Society of Washington, 1988, 55(1): 1-9.
[2]Fang Yusheng, Zhao Jun, Zhuo Kan. Description of Bursaphelenchus dongguansis sp.n.in China(Nematoda: Aphelenchoididae)[J]. Journal of Huazhong Agricultural University, 2002, 21(2): 109-110.
[3]Huang R E, Ye J R. Seinura chuandaoi sp.n.and S.xiaoqinae sp.n.(Nematoda: Seinuridae) from pinewood in China[J]. Nematology, 2006, 8(6): 749-759.
[4]王宏毅,张绍升. 中国马尾松木上伞滑刃属线虫新种记述[J]. 动物分类学报,2007,32(4):906-912.
[5]Kan Zhuo, Xundong Li, Danlei Li, et al. Bursaphelenchus uncispicularis sp.n.(Nematoda:Parasitaphelenchidae) from Pinus yunnanensis in China[J]. Nematology, 2007, 9(2): 237-242.
[6]汤坚,叶建仁,陈凤毛,等. 松树体内寄生线虫种类及其致病性[J]. 南京林业大学学报:自然科学版,2008,32(3):112-116.
[7]黄任娥,叶建仁,潘宏阳,等. 感染松材线虫病松树滑刃目线虫调查[J]. 植物病理学报,2008,38(2):136-146.
[8]朱丽梅,吴小芹,蒋萍. 细菌XSJS3对松材线虫杀线活性的测定[J]. 南京林业大学学报:自然科学版,2008,32(1):79-82.
[9]朱丽梅,吴小芹,徐旭凌. 松材线虫拮抗细菌的筛选和鉴定[J]. 南京林业大学学报:自然科学版,2008,32(3):91-94.
[10]黄庆生,王加启. 16SrRNA/rDNA序列分析技术在瘤胃细菌微生态系统研究中的应用[J]. 中国畜牧兽医,2003,30(1):7-11.
[11]蒋萍. 马尾松叶面微生物区系及松枯梢病菌拮抗菌的研究[D]. 南京:南京林业大学,2007.
[12]刘静,王军,姚建铭,等. 枯草芽孢杆菌JA抗菌物特性的研究及抗菌肽的分离纯化[J]. 微生物学报,2004,44(4):511-513.
[13]陈士云,杨宝玉,高梅影,等. 一株抑制油菜核盘菌菌核形成的解淀粉芽孢杆菌[J]. 应用与环境生物学报,2005,11(3):373-376.
[14]Chiou A L, Wu W S. Formulation of Bacillus amyloliquefacines B190 for control of lily grey mould(Botrytis elliptica)[J]. J Phytopathol, 2003, 151: 13-18.
[15]Yu G Y, Sinclair J B, Hartman G L, et al. Production of iturin A by Bacillus amyloliquefaciens suppressing Rhizoctonia solani[J]. Soil Biology & Biochemistry, 2002, 34: 955-963.
[16]Wang S L, Shi I L, Liang T W, et al. Purification and characterization of two antifungal chitinases extracellularly produced by Bacillus amyloliquefaciens V656 in a shrimp and crab shell powder medium[J]. J Agric Food Chem, 2002, 50(8): 2241-2248.

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备注/Memo

备注/Memo:
收稿日期：2009-02-17修回日期：2009-06-03基金项目：国家林业局“948”项目(2003-4-38)作者简介：朱丽梅(1972—)，副教授，博士。*吴小芹(通讯作者)，教授，研究方向为森林保护学。Email: xqwu@njfu.com.cn。引文格式：朱丽梅,吴小芹,徐旭凌. 细菌JKJS3对松树线虫的杀线活性及菌种的分子鉴定[J]. 南京林业大学学报:自然科学版,2009,33(6):49-52.
更新日期/Last Update: 2009-11-30