[1]孔令营,郭道森,赵博光,等.荧光假单胞菌Pseudomonas fluorescens GcM51A菌株的胞外木素过氧化物酶的部分纯化和鉴定[J].南京林业大学学报(自然科学版),2010,34(02):151-152.[doi:10.3969/j.jssn.1000-2006.2010.02.036]
 KONG Ling ying,GUO Dao sen,ZHAO Bo guang,et al.Partial purification and characterization of extracellular lignin peroxidase from Pseudomonas fluorescens GcM51A[J].Journal of Nanjing Forestry University(Natural Science Edition),2010,34(02):151-152.[doi:10.3969/j.jssn.1000-2006.2010.02.036]
点击复制

荧光假单胞菌Pseudomonas fluorescens GcM51A菌株的胞外木素过氧化物酶的部分纯化和鉴定
分享到:

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
34
期数:
2010年02期
页码:
151-152
栏目:
国际会议学术论文选登
出版日期:
2010-03-30

文章信息/Info

Title:
Partial purification and characterization of extracellular lignin peroxidase from Pseudomonas fluorescens GcM51A
作者:
孔令营1郭道森1赵博光2李荣贵1
1.青岛大学生物系,山东青岛266071;2.南京林业大学森林资源与环境学院,江苏南京210037
Author(s):
KONG Lingying1 GUO Daosen1 ZHAO Boguang2 LI Ronggui1
1.Department of Biology, Qingdao University, Qingdao 266071, China; 2.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China
关键词:
荧光假单胞菌GcM51A胞外木素过氧化物酶
分类号:
S763
DOI:
10.3969/j.jssn.1000-2006.2010.02.036
文献标志码:
A
摘要:
荧光假单胞菌GcM51A是松材线虫携带的一种致病菌。在GcM51A的培养液中发现有木质素过氧化物酶的活性,同时显示很强的毒性。通过对其胞外木质素过氧化物酶的酶学性质的初步研究发现:其最适温度为35 ℃,最适pH为4.0。在15~35 ℃、pH为6.0~9.0范围内,酶活保持相对稳定。NH2OH·HCl和KCN对酶活的抑制作用分别达到88 %和57 %,而NaN3对酶活的抑制作用只有15 %左右。Ca2+、Mn2+ 和Fe3+对其活性有增强作用,但是Hg2+ 和 Zn2+却有中等的抑制作用。KCN 和NH2OH·HCl抑制其酶活性分别达57 %、88 %,而NaN3的抑制率仅为15 %。吸收光谱显示该酶在406 nm处有一个Soret峰,加入1 mmol/L Na2S2O4后,吸光度出现了明显的下降,但Soret峰没有显著移动。

参考文献/References:

[1]Morimoto K, Iwaslki A. Role of Monochamus alternatus(Coleoptera: Cerambycidae) as a vector of Bursaphelenchus lignicolus(Nematoda: Aphelenchoididae)[J]. J Jpn For Soc, 1972, 54(6): 177-183.
[2]Yano M. Investigation on pine death in Nagasaki prefecture[J]. SanrinKoho, 1913, 4(Suppl.): 1-14.
[3]Zhao B G, Futai K, Sutherland J R, et al. Pine Wilt disease[M]. New York: Springer Publishing Company, 2008.
[4]Myers R F. Pathogenesis in pine wilt caused by pine wood nematode, Bursaphelenchus xylophilus[J]. J Nematol, 1988, 20(2): 236-244.
[5]Kawazu K, Kaneko N. Asepsis of the pine wood nematode isolate OKD3 causes it to lose its pathogenicity[J]. Jap J Nematol, 1997, 27(2): 76-80.
[6]Zhao B G, Wangh L, HanS F et al. Distribution and pathogenicity of bacteria species carried by Bursaphelenchus xylophilus in China[J]. Nematology, 2003, 5(6): 899-906.
[7]Guo Q, Guo D, Zhao B, et al. Two cyclic dipeptides from Pseudomonas fluorescens GcM51A carried by the pine wood nematode and their toxicities to Japanese black pine suspension cells and seedlings in vitro[J]. J Nematol, 2007, 39(3): 243-247.
[8]Guo D, Zhao B, Li R, et al. Purification of flagellin of Pseudomonas fluorescens GcM51A carried by the pine wood nematote, Bursaphelenchus xylophilus, and its in vitro toxicity to a suspension of cells of Pinus thunbergii[J]. Russian Journal of Nematology, 2008, 16(2): 151-157.
[9]Kersten P J. Glyoxal oxidase of Phanerochaete chrysosporium: its characterization and activation by lignin peroxidase[J]. Proc Natl Acad Sci USA, 1990, 81: 2280-2284.
[10]Hammel K E, Jensen K A, Mozuch M D, et al. Ligninolysis by a purified lignin peroxidase[J]. J Biol Chen, 1993, 268: 12274-12281.
[11]Hammel K E. Extracellular free radical biochemistry of ligninollytic fungi[J]. New J Chem, 1996, 20: 195-198.
[12]Harvey P, Schoemaker H E, Pslmer J M.Veratryl alcohol as a mediator and the role of radical cation in lignin biodegradation by Phanerochaete chrysosporium[J]. FEBS Letters, 1986, 195: 242-246.
[13]Tien M, Kirk T K. Lignindegrading enzyme from Phanerochaete chrysosporium: purification, characterization, and catalytic properties of a unique H202requiring oxygenase[J]. Proc Natl Acad Sci USA, 1984, 81: 2280-2284.
[14]Hartree E F. Determination of protein: a modification of the lowry method that gives a linear photometric response[J]. Analytical Biochemistry, 1972, 48: 422-427.第34卷第2期 2010年3月南京林业大学学报(自然科学版) Journal of Nanjing Forestry University (Natural Science Edition)Vol.34, No.2 Mar., 2010

更新日期/Last Update: 2010-05-14