[1]蒋泽平,肖小君,何开跃*.NaCl胁迫对构树、光叶楮生理及细胞结构的影响[J].南京林业大学学报(自然科学版),2010,34(03):077-82.[doi:10.3969/j.jssn.1000-2006.2010.03.016]
 JIANG Ze ping,XIAO Xiao jun,HE Kai yue*.Effects of NaCl stress on physiological characteristic and cell ultrastructure in leaves of two Broussonetia papyrifera varieties[J].Journal of Nanjing Forestry University(Natural Science Edition),2010,34(03):077-82.[doi:10.3969/j.jssn.1000-2006.2010.03.016]
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NaCl胁迫对构树、光叶楮生理及细胞结构的影响
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
34
期数:
2010年03期
页码:
077-82
栏目:
研究论文
出版日期:
2010-06-29

文章信息/Info

Title:
Effects of NaCl stress on physiological characteristic and cell ultrastructure in leaves of two Broussonetia papyrifera varieties
作者:
蒋泽平1肖小君23何开跃2*
1.江苏省林业科学研究院,江苏南京211153;2.南京林业大学森林资源与环境学院,江苏南京210037; 3.陕西汉中市农业科学研究所,陕西汉中723000
Author(s):
JIANG Zeping1 XIAO Xiaojun23 HE Kaiyue2*
1.Forestry of Academy Jiangsu Province, Nanjing 211153, China; 2.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China; 3.Hanzhong Agricultural Science Institute Shaanxi Province, Hanzhong 723000, China
关键词:
NaCl胁迫构树光叶楮生理特性细胞超微结构耐盐性
Keywords:
NaCl stress Broussonetia papyrifera Vent B.kazinoki Sied physiological characteristic cell ultrastructure salt tolerance
分类号:
S718
DOI:
10.3969/j.jssn.1000-2006.2010.03.016
文献标志码:
A
摘要:
以1年生构树和光叶楮幼苗为材料,研究了其细胞膜透性、MDA(丙二醛)含量、SOD(超氧化物歧化酶)和POD(过氧化物酶)活性对不同浓度NaCl胁迫的反应,并观察了细胞超微结构的变化。结果显示:(1)随NaCl浓度的升高及胁迫时间的延长,构树和光叶楮膜透性和MDA含量显著增加;而SOD和POD活性先增加后下降,抗氧化酶活性(SOD和POD)之间协调变化有利于清除活性氧,维持活性氧代谢平衡,保护膜结构。(2)低浓度NaCl处理下(质量分数为0.1 %和0.2 %)构树和光叶楮叶片的细胞超微结构未受到伤害,同时可观察到线粒体增多的现象;随着盐浓度的增加,细胞超微结构变化较大,0.4 % NaCl胁迫时构树和光叶楮叶片细胞器变形严重,液泡增大明显,且构树细胞核明显固缩,核物质凝聚,线粒体结构模糊,细胞结构受到严重伤害。综合分析认为:光叶楮的耐盐性较强。
Abstract:
The membrane permeability, MDA contents, SOD and POD activities and cell ultrastructure of the oneyear seedlings of two Broussonetia papyrifera varieties under different NaCl treatments were studied. The results were as follows: (1)With an increase in NaCl stress and treating time, the membrane permeability and MDA contents in both cultivars increased considerably. The membrane permeability and MDA contents in the B.papyrifera cultivar increased more quickly thus suffered greater damage at higher NaCl concentrations. The activities of SOD and POD increased firstly and decreased later for both cultivars. The coordinated changes of activities among the antioxidant enzymes of SOD and POD could scavenge ROS and maintain a balance of ROS accumulation in cells to protect membrane structure. (2)The cell ultrastructure in leaves of two cultivars didn’t destroy under 0.1 % and 0.2 % NaCl and mitochondrial grew in number. With the increasing NaCl stress, the cell ultrastructure changed remarkably, the cellular organs for both cultivars became deformed and vacuole augmented under NaCl. Besides, the nucleolus diminished obviously and nucleolus matter agglomerated and the mitochondria structure was indistinct. Altogether, we concluded the B.kazinoki cultivar had greater salttolerance.

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备注/Memo

备注/Memo:
收稿日期:2009-06-29修回日期:2010-01-26基金项目:“十一五”国家科技支撑计划(2006BAD09A04-01)作者简介:蒋泽平(1963—),研究员。*何开跃(通信作者),副教授。Email: hekaiy2002@yahoo.com.cn。引文格式:蒋泽平,肖小君,何开跃. NaCl胁迫对构树、光叶楮生理及细胞结构的影响[J]. 南京林业大学学报:自然科学版,2010,34(3):77-82.
更新日期/Last Update: 2010-06-29