[1]龚明,王进*.甲胺磷影响下土壤微生物群落结构的动态平衡[J].南京林业大学学报(自然科学版),2011,35(03):001.[doi:10.3969/j.jssn.1000-2006.2011.03.002]
 GONG Ming,WANG Jin*.The dynamic equilibrium of soil microbial community structure affected by methamidophos[J].Journal of Nanjing Forestry University(Natural Science Edition),2011,35(03):001.[doi:10.3969/j.jssn.1000-2006.2011.03.002]
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甲胺磷影响下土壤微生物群落结构的动态平衡
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
35
期数:
2011年03期
页码:
001
栏目:
研究论文
出版日期:
2011-06-13

文章信息/Info

Title:
The dynamic equilibrium of soil microbial community structure affected by methamidophos
作者:
龚明王进*
南京大学生命科学学院,医药生物技术国家重点实验室,江苏南京210093
Author(s):
GONG Ming WANG Jin*
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China
关键词:
土壤微生物群落甲胺磷生物多样性磷脂脂肪酸
Keywords:
soil microbial community methamidophos biological diversity phospholipid fatty acid (PLFA)
分类号:
S7188
DOI:
10.3969/j.jssn.1000-2006.2011.03.002
文献标志码:
A
摘要:
采用磷脂脂肪酸(PLFA)作为一种微生物分子标靶,研究不同浓度甲胺磷(C2H8ONPS)输入对土壤微生物群落的影响。结果表明:与对照土壤相比,高浓度甲胺磷分别使细菌PLFAs显著提高 0.85 nmol/g,而使真菌PLFAs显著下降 0.87 nmol/g。甲胺磷并没有使土壤微生物总量发生显著变化,真菌丰度下降和抵抗性细菌群落上升形成了土壤微生物群落结构的动态平衡。分析认为,由甲胺磷输入而产生的H+可能导致这一微生物群落结构的变化。
Abstract:
Phospholipid fatty acid (PLFA) as microbial molecular target was used to investigate the impact of different inputs of methamidophos (C2H8ONPS) on soil microbial community. Compared to the control soil, there was an increase of 0.85 nmol/g in the bacterial PLFAs and a decrease of 0.87 nmol/g in the fungi PLFAs, respectively. Our results represented that microbial biomass was not affected significantly by methamidophos. A significant reduction in fungal abundance and a selection of resistant bacterial population constituted the dynamic equilibrium of soil microbial community structure. The changes of soil microbial community structure might be due to H+ provided by methamidophos.

参考文献/References:

[1]Das A C,Mukherjee D. Soil application of insecticides influences microorganisms and plant nutrients [J]. Applied Soil Ecology, 2000, 14:55-62.
[2]Moorman T B. A review of pesticide effects on microorganisms and microbial processes related to soil fertility [J]. Journal of Production Agriculture, 1989, 2:14-23.
[3]MartinezToledo M V, Salmeron V, GonzalezLopez J. Effect of an organophosphorus insecticide, phenophos on agricultural soil microflora [J]. Chemosphere, 1992, 24:71-80.
[4]Meister R T. Farm Chemicals Handbook ′95 [M]. Willoughby OH: Meister Publishing Company, 1995.
[5]Kidd H, James D R. Agrochemicals Handbook [M]. Third Edition. Cambridge, England: Royal Society of Chemistry, 1994.
[6]Gary N E, Lorenzen K. Effect of methamidophos on honey bees (Hymenoptera: Apidae) during alfalfa pollination [J]. Journal of Economic Entomology, 1989, 82:1067-1072.
[7]White D C. Chemical ecology: possible linkage between macroand microbial ecology [J]. Oikos, 1997, 74:177-184.
[8]王勐骋,杨永华,臧红兵.利用PLFA、CLPPs和ARDRA标记分析甲胺磷对土壤微生物群落的影响[J].生态学杂志,2006,25(6):640-645.
[9]Wan Abdullah W Y, Aminuddin B Y, Zulkifli M. Modelling pesticide and nutrient transport in the Cameron highlands, Malaysia agroecosystems [J]. Water, Air, and Soil Pollution: Focus, 2005, 5:115-123.
[10]Rayment G E, Higginson F R. Australian Laboratory Handbook of Soil and Water Chemical Methods [M]. Melbourne: Inkata Press, 1992.
[11]White D C, Ringelberg D B. Signature Lipid Biomarker Analysis [S]. Burlage R S, Atlas R, Stahl D, et al. Techniques in Microbial Ecology. New York: Oxford University Press, 1998.
[12]Bossio D A, Scow K M, Gunapala N, et al. Determinants of soil microbial communities: effects of agricultural management, season, and soil type on phospholipid fatty acid profiles [J]. Microbial Ecology, 1998, 36:1-12.
[13]Frostega°rd A, Ba°a°th E, Tunlid A. Shifts in the structure of soil microbial communities in limed forests as revealed by phospholipids fatty acid analysis [J]. Soil Biology and Biochemistry, 1993, 25: 723-730.
[14]Federle T W. Microbial distribution in soilnew techniques [C]// Megusar F, Gantar M: Perspectives in Microbial Ecology: Proceedings of the Fourth International Symposium on Microbial Ecology. Ljubljana, Slovenia: Slovene Society for Microbiology, 1986.
[15]Bardgett R D, Hobbs P J, Frostega°rd A. Changes in soil fungal:bacterial biomass ratios following reductions in the intensity of management of an upland grassland [J]. Biology and Fertility of Soils, 1996, 22:261-264.
[16]Fierer N, Schimel J P, Holden P A. Variations in microbial community composition through two soil depth profiles [J]. Soil Biology and Biochemistry, 2003, 35:167-176.
[17]Barnhart C L, Vestal R. Effect of environmental toxicant on metabolic activity of natural microbial communities [J]. Applied Environmental Microbiology, 1983, 46:970-977.
[18]Bhuyan S, Sreedharan B, Adhya T K, et al. Enhanced biodegradation of (g-HCH) in HCH (commerial) acclimatized flooded soil: factors affecting its development and persistence [J]. Pesticide Science, 1993, 38:49-55.
[19]EPA. Methamidophos: Revision of EFED Risk Assessment for the reregistration Eligibility Decision (RED) Document to Include Registrants Comments [M]. Washington DC: Office of Prevention, Pesticides and Toxic Substances, 1998.
[20]Kunji E R, Robinson A J. Coupling of proton and substrate translocation in the transport cycle of mitochondrial carriers [J]. Curr Opin Struct Biol, 2010, 20:440-447.
[21]朱南文,胡茂林,高廷耀.甲胺磷对土壤微生物活性的影响[J].农业环境保护,1999,18(1):4-7.
[22]Pennanen T, Perkimki J, KiikkilO, et al. Prolonged, simulated acid rain and heavy metal deposition: separated and combined effects on forest soil microbial community structure [J]. FEMS Microbiology Ecology, 1998, 27:291-300.
[23]Zeller V, Bardgett R D, Tappeiner U. Site and management effects on soil microbial properties of subalpine meadows: a study of land abandoment along a northsouth gradient in European Alps [J]. Soil Biology and Biochemistry, 2001, 33:639-649.
[24]Wu Y, Ding N, Wang G, et al. Effects of different soil weights, storage times and extraction methods on soil phospholipid fatty acid analyses [J]. Geoderma, 2009, 150:171-178.

备注/Memo

备注/Memo:
收稿日期:2010-12-28修回日期:2011-04-06基金项目:国家自然科学基金项目(90813035, 30890044)作者简介:龚明(1978—),博士生。*王进(通信作者),教授。Email: lilysearch@163.com。引文格式:龚明,王进. 甲胺磷影响下的土壤微生物群落结构动态平衡[J]. 南京林业大学学报:自然科学版,2011,35(3):6-10.
更新日期/Last Update: 2011-06-13