[1]焦虎美,时留新*.腐浆中细菌的鉴定及其生物膜活性研究[J].南京林业大学学报(自然科学版),2012,36(02):077-80.[doi:10.3969/j.jssn.1000-2006.2012.02.016]
 JIAO Humei,SHI Liuxin*.Identification of bacterias from the slime and the study on their bioflim activity[J].Journal of Nanjing Forestry University(Natural Science Edition),2012,36(02):077-80.[doi:10.3969/j.jssn.1000-2006.2012.02.016]
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腐浆中细菌的鉴定及其生物膜活性研究
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
36
期数:
2012年02期
页码:
077-80
栏目:
研究论文
出版日期:
2012-04-10

文章信息/Info

Title:
Identification of bacterias from the slime and the study on their bioflim activity
作者:
焦虎美时留新*
南京林业大学轻工科学与工程学院,江苏 南京 210037
Author(s):
JIAO HumeiSHI Liuxin*
College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing 210037,China
关键词:
腐浆细菌 鉴定 生物膜活性
Keywords:
bacteria in slime identification bioflim activity
分类号:
TS78; X703
DOI:
10.3969/j.jssn.1000-2006.2012.02.016
文献标志码:
A
摘要:
纸机运转过程中的腐浆障碍主要是由系统内微生物所引起的。从UPM常熟纸厂PM2纸机机架的腐浆里筛选得到10株细菌N1、N2……N10,通过对其形态特征、生理生化特性以及16S rRNA 序列分析,初步鉴定N1、N3为鞘氨醇单孢菌(Sphingomonas azotifigens)、N2为腐败假单孢菌(Pseudomonas putrefaciens)、N4为未培养细菌(uncultured bacterium)、N5为Filimonas lacunae、N6为Asticcacaulis excentricus、N7为少动假单孢菌(Pseudomonas paucimobilis)、N8为Siphonobacter aquaeclarae、N9为不解糖鞘氨醇单孢菌(Sphingomonas pruni)、N10为Flectobacillus Larkin。将分离后的10株细菌分别回接到含细小纤维的回接试样中,检测腐浆细菌的生物膜对细小纤维的絮聚情况,结果显示N5、N8、N10对细小纤维有很大的絮凝沉淀作用,由此推断这3株细菌的生物膜活性强,是腐浆中的有害细菌。
Abstract:
The slime pulp, causing much trouble for the pulp and paper making industry, is usually produced by bacterium. Ten kinds of bacteria including N1, N2,…,N10 were screened from the slime hanging on the PM2 paper machine at UPM. They were identified as Sphingomonas azotifigens(N1, N3), Pseudomonas putrefaciens(N2), uncultured bacterium(N4), Filimonas lacunae (N5), Asticcacaulis excentricus(N6), Pseudomonas paucimobilis(N7), Siphonobacter aquaeclarae(N8), Sphingomonas pruni(N9), Flectobacillus Larkin(N10)through the morphological observation, physiological & chemical tests and 16S rRNA sequence analysis. Inoculate these bacteria to the simulative white water system and detect whether the slime was produced. It was proved that N5,N8,N10 were the key bacteria for the slime.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2011-07-04 修回日期:2011-11-22 基金项目:南京林业大学科技创新基金项目(X09-030-5) 第一作者:焦虎美,硕士生。*通信作者:时留新,副教授。E-mail:shiliuxin@njfu.edu.cn。引文格式:焦虎美, 时留新.
更新日期/Last Update: 2012-04-10