[1]任淑娟,喻方圆*,徐丽萍.引发对马尾松种子细胞周期和发芽率的影响[J].南京林业大学学报(自然科学版),2012,36(05):051-55.[doi:10.3969/j.jssn.1000-2006.2012.05.009]
 REN Shujuan,YU Fangyuan*,XU Liping.Effects of priming on cell cycle and germination of Pinus massoniana seeds[J].Journal of Nanjing Forestry University(Natural Science Edition),2012,36(05):051-55.[doi:10.3969/j.jssn.1000-2006.2012.05.009]
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引发对马尾松种子细胞周期和发芽率的影响
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
36
期数:
2012年05期
页码:
051-55
栏目:
研究论文
出版日期:
2012-09-30

文章信息/Info

Title:
Effects of priming on cell cycle and germination of Pinus massoniana seeds
作者:
任淑娟喻方圆* 徐丽萍
南京林业大学森林资源与环境学院,江苏南京21003
Author(s):
REN Shujuan YU Fangyuan* XU Liping
College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China
关键词:
马尾松种子引发处理细胞周期种子萌发PEG 8000流式细胞术
Keywords:
Pinus massoniana seedspriming treatmentcell cycleseed germination PEG 8000 flow cytometry
分类号:
S722
DOI:
10.3969/j.jssn.1000-2006.2012.05.009
文献标志码:
A
摘要:
采用流式细胞术研究不同引发处理下马尾松种子胚细胞DNA含量的差异,测定不同引发处理下马尾松种子的发芽率。结果表明:(1)PEG 8000溶液引发处理能促进胚细胞DNA的复制,4倍体细胞的比例增加,其中以20 %PEG 8000溶液引发3~4 d的处理对种子胚细胞DNA合成的促进作用最明显。(2)胚细胞增殖指数最高的引发处理是20 %PEG 8000溶液在15 ℃时引发3~4 d。(3)较高浓度的PEG 8000溶液和较长的引发时间有利于提高马尾松种子发芽率。(4)在一定范围内,胚细胞增殖指数与种子发芽率呈显著正相关。
Abstract:
In order to reveal the cytological mechanism of promoting seed germination through seed priming, the differences of DNA content in embryonic cell of Pinus massoniana seeds after different priming treatments were studied by using flow cytometry, and seed germination percentage was also determined. The results showed that: (1) DNA replication in embryonic cell could be enhanced by PEG 8000 solution pretreatment, the ratio of tetraploid cells increased, the best treatment was a regime treated for 3-4 days using 20 % PEG 8000 solution. (2) The best priming treatment for increasing cell proliferation index was a regime treated in 20 % PEG 8000 solution for 3-4 days at 15 ℃. (3) Higher PEG 8000 concentration and longer pretreatment time were benefit for increasing germination percentage. (4) In a certain extent, there was a significant positive correlation between the cell proliferation index and seed germination percentage.

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备注/Memo

备注/Memo:
收稿日期:2011-12-04修回日期:2012-05-25
基金项目:江苏省普通高校研究生科研创新计划资助项目(苏教研〔2009〕8号)
第一作者:任淑娟,硕士生。*通信作者:喻方圆,教授。E-mail: fyyu@njfu.edu.cn。
引文格式:任淑娟,喻方圆,徐丽萍. 引发对马尾松种子细胞周期和发芽率的影响[J]. 南京林业大学学报:自然科学版,2012,36(5):51-55.
更新日期/Last Update: 2012-09-30