[1]马晓乾,赵红盈,周 琦,等.光肩星天牛产卵分泌物的化学成分分析[J].南京林业大学学报(自然科学版),2013,37(01):083-86.[doi:10.3969/j.issn.1000-2006.2013.01.014]
 MA Xiaoqian,ZHAO Hongying,ZHOU Qi,et al.Composition analysis of the oviposition secretion of Anoplophora glabripennis[J].Journal of Nanjing Forestry University(Natural Science Edition),2013,37(01):083-86.[doi:10.3969/j.issn.1000-2006.2013.01.014]
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光肩星天牛产卵分泌物的化学成分分析
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
37
期数:
2013年01期
页码:
083-86
栏目:
研究论文
出版日期:
2013-01-15

文章信息/Info

Title:
Composition analysis of the oviposition secretion of Anoplophora glabripennis
作者:
马晓乾1赵红盈1周 琦1邓 勋1宋小双1遇文婧1范海娟2
1.黑龙江省林业科学研究院,黑龙江 哈尔滨 150040; 2.东北林业大学林学院,黑龙江 哈尔滨 150040
Author(s):
MA Xiaoqian1ZHAO Hongying1ZHOU Qi1DENG Xun1SONG Xiaoshuang1YU Wenjing1FAN Haijuan2
1.Heilongjiang Academy of Forestry, Harbin 150040,China; 2. College of Forestry, Northeast Forestry University, Harbin 150040,China
关键词:
光肩星天牛 产卵分泌物 蛋白质 可溶性糖 游离氨基酸
Keywords:
Anoplophora glabripennis oviposition secretion protein soluble sugar free amino acids
分类号:
S763.38
DOI:
10.3969/j.issn.1000-2006.2013.01.014
文献标志码:
A
摘要:
通过室内饲养产卵期光肩星天牛成虫并采集其产卵分泌物,对光肩星天牛产卵分泌物进行组分分析。利用烘干法、Bradford法、蒽酮比色法、茚三酮试剂显色法、SDS-PAGE对其组分中水、蛋白质、可溶性糖、游离氨基酸的含量及蛋白质组分进行了测定。结果表明:光肩星天牛产卵分泌物组分中含水量为81.98%,蛋白质含量为4.14%,可溶性糖及游离氨基酸的含量依次为0.51%和0.06%(均为质量分数),蛋白质组分中主要蛋白的分子质量为107.1、86.7、62.2、47.5、39.0 ku,其中107.1~86.7 ku与47.5 ku为强谱带区。
Abstract:
To analyze the composition of Anoplophora glabripennis oviposition secretion, A.glabripennis were kept indoors during the spawning period, and oviposition secretion of which was collected. The oven drying method, Bradford method, anthrone colorimetry, Ninhydrin reagent colorimetric method and SDS-PAGE were used to analyze and determinate the fractions of water, protein, soluble sugar and free amino acid content and protein fractions in oviposition secretion. The results showed that oviposition secretion components of A.glabripennis included water(81.98%), protein(4.14%), soluble sugar(0.51%)and free amino acid(0.06%). The molecular weight of main protein components were 107.1 ku,86.7 ku,62.2 ku,47.5 ku and 39.0 ku. The strong spectral bands were 107.1 ku to 86.7 ku and 47.5 ku.

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备注/Memo

备注/Memo:
收稿日期:2012-03-20 修回日期:2012-10-15
基金项目:黑龙江省青年科学基金项目(QC2009C64)
第一作者:马晓乾,助理研究员。E-mail:369086324@qq.com。
引文格式:马晓乾,赵红盈,周琦,等. 光肩星天牛产卵分泌物的化学成分分析[J]. 南京林业大学学报:自然科学版,2013,37(1):83-86.
更新日期/Last Update: 2013-01-15