[1]匙明强,王焱,叶建仁*,等.水杉赤枯病病原形态及分子鉴定[J].南京林业大学学报(自然科学版),2013,37(05):075-80.[doi:10.3969/j.issn.1000-2006.2013.05.015]
 CHI Mingqiang,WANG Yan,YE Jianren*,et al.Morphological and molecular identification of Metasequoia red blight[J].Journal of Nanjing Forestry University(Natural Science Edition),2013,37(05):075-80.[doi:10.3969/j.issn.1000-2006.2013.05.015]
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水杉赤枯病病原形态及分子鉴定
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
37
期数:
2013年05期
页码:
075-80
栏目:
研究论文
出版日期:
2013-09-30

文章信息/Info

Title:
Morphological and molecular identification of Metasequoia red blight
文章编号:
1000-2006(2013)05-0075-06
作者:
匙明强1 王焱2 叶建仁1* 张岳峰2
1.南京林业大学森林资源与环境学院,江苏省有害生物入侵预防与控制重点实验室,江苏南京210037;
2.上海市林业总站,上海200072
Author(s):
CHI Mingqiang1 WANG Yan2 YE Jianren1* ZHANG Yuefeng2
1.Jiangsu Key Laboratory for Prevention and Control of Invasive Species, College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China;
2.Shanghai Forestry Station, Shanghai 200072, China
关键词:
水杉赤枯病细极链格孢异角状拟盘多毛孢形态鉴定分子鉴定
Keywords:
Metasequoia red blight Alternaria tenuissima Pestalotiopsis heterocornis morphological identification molecular identification
分类号:
S763
DOI:
10.3969/j.issn.1000-2006.2013.05.015
文献标志码:
A
摘要:
对上海、南京地区的水杉赤枯病(Metasequoia red blight)的发生情况进行了调查和分析,并采用形态学和分子生物学结合的方法对该病的病原菌进行了鉴定。结果表明:单行种植和单棵零星分布的水杉感病程度相对于已经郁闭成林的水杉要严重得多;用作行道树的水杉抗病能力较弱,发病情况相对于农田中的水杉较为严重;生长势弱的水杉植株容易发生赤枯病,这一现象在行道树中表现尤为明显。通过对水杉赤枯病病原菌分离培养、人工接种试验、分离菌形态学观察及分子生物学分子鉴定,水杉赤枯病的病原菌被鉴定为细极链格孢(Alternaria tenuissima)和异角状拟盘多毛孢(Pestalotiopsis heterocornis)。
Abstract:
The occurrence regularity of Metasequoia red blight disease in Shanghai and Nanjing were investigated and analysed. The pathogens of the disease were identified with morphological and molecular methods. Results showed: Metasequoia of single row or sporadic distribution was more sensitive than that with a higher canopy density. The resistance of Metasequoia that used as street tree was weak, and the disease was more serious than that planted in farm. The Metasequoia with a poor growth potential was more likely to be infected, especially the ones used as street trees. By isolation and culture of diseased tissue, artificial inoculation test, morphological observation and molecular identification by ITS analysis of the isolated strain, the pathogen of Metasequoia red blight was identified as Alternaria tenuissima and Pestalotiopsis heterocornis.

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相似文献/References:

[1]朱克恭.水杉赤枯病病原初探[J].南京林业大学学报(自然科学版),1987,11(03):029.[doi:10.3969/j.jssn.1000-2006.1987.03.004]
 Zhu Kegong.A PRELIMINARY STUDAY OF ETIOLOGY OF METASEQUOIA BLIGHT[J].Journal of Nanjing Forestry University(Natural Science Edition),1987,11(05):029.[doi:10.3969/j.jssn.1000-2006.1987.03.004]

备注/Memo

备注/Memo:
收稿日期:2012-05-05修回日期:2013-06-05 基金项目:上海市科技兴农重点攻关项目[沪农科攻字(2009)第9-1号] 第一作者:匙明强,硕士。 *通信作者:叶建仁,教授。 Email: jrye@njfu.edu.cn。 引文格式:匙明强, 王焱, 叶建仁,等. 水杉赤枯病病原形态及分子鉴定[J]. 南京林业大学学报:自然科学版,2013,37(5):75-80.
更新日期/Last Update: 2013-09-30