[1]陈梦义,徐 梅,钱 路,等.DNA条形码试剂盒检测技术在墨天牛鉴定中的应用[J].南京林业大学学报(自然科学版),2015,39(06):007-11.[doi:10.3969/j.issn.1000-2006.2015.06.002]
 CHEN Mengyi,XU Mei,QIAN Lu,et al.The application of DNA barcoding identification kit technology to Monochamus Dejean[J].Journal of Nanjing Forestry University(Natural Science Edition),2015,39(06):007-11.[doi:10.3969/j.issn.1000-2006.2015.06.002]
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DNA条形码试剂盒检测技术在墨天牛鉴定中的应用
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
39
期数:
2015年06期
页码:
007-11
栏目:
专题报道
出版日期:
2015-11-30

文章信息/Info

Title:
The application of DNA barcoding identification kit technology to Monochamus Dejean
文章编号:
1000-2006(2015)06-0007-05
作者:
陈梦义12徐 梅3钱 路3安榆林3郝德君12*
1.南京林业大学南方现代林业协同创新中心,江苏 南京 210037;
2.南京林业大学林学院,江苏 南京 210037;
3.江苏出入境检验检疫局,江苏 南京 210001
Author(s):
CHEN Mengyi12 XU Mei3 QIAN Lu3 AN Yulin3 HAO Dejun12*
1. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China;
2. College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
3. Jiangsu Entry-exit Inspection and Quarantine Bureau, Nanjing 210001, China
关键词:
墨天牛属 COⅠ基因 DNA条形码试剂盒 标记性碱基
Keywords:
Monochamus COⅠ gene DNA barcoding identification kit technology specific site quarantine
分类号:
S763
DOI:
10.3969/j.issn.1000-2006.2015.06.002
文献标志码:
A
摘要:
为了对进境口岸截获的俄罗斯木材中的墨天牛属(非中国种)害虫进行准确、快速鉴定,笔者利用DNA 条码试剂盒检测技术,基于墨天牛线粒体COⅠ(线粒体细胞色素氧化酶亚基I)基因设计一对巢式引物,对截获墨天牛属中的8 个种进行COⅠ扩增,将扩增序列上传至有害生物信息网站,依据碱基位点在不同种间的排列和组成的差异,获得不同种在相应位点上的特有碱基,即标记性碱基,以此作为区分墨天牛种的依据。通过这种墨天牛属DNA 条码试剂盒检测技术,可为口岸检疫鉴定提供有效的技术手段。
Abstract:
In order to rapidly and accurately identify the Monochamus of wood from Russian, we employed DNA barcode detection kit technology and designed a pair of nested primers based on mitochondrial COⅠ gene(mitochondrial cytochrome oxidase subunit I). Furthermore, COⅠ gene sequence of eight Monocharnus species was amplificated, and uploaded to pest information site. According to differences of arrangement and composition among these species at base sites, species unique bases was got in the corresponding sites, which is named specific site to distinguish this species. In this way, we developed the DNA barcoding identification kit technology to Monochamus species, which will provide a new way for port quarantine identification.

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相似文献/References:

[1]安榆林,刁彩华,朱宏斌,等.墨天牛属三个近缘种的RAPD分析[J].南京林业大学学报(自然科学版),1998,22(04):035.[doi:10.3969/j.jssn.1000-2006.1998.04.008]
 An Yulin Diao Caihua Zhu Hongbing Jiao Guoyao.RAPD ASSESSMENT OF THREE SIBLING SPECIES OFMONOCHAMUS GUER (COLEOPTERA: CERAMBYCIDAE)[J].Journal of Nanjing Forestry University(Natural Science Edition),1998,22(06):035.[doi:10.3969/j.jssn.1000-2006.1998.04.008]

备注/Memo

备注/Memo:
收稿日期:2014-12-10 修回日期:2015-09-09
基金项目:“十二五”国家科技支撑计划(2012BAK11B03); 国家自然科学基金项目(31170606); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:陈梦义,硕士生。*通信作者:郝德君,教授。E-mail: dejunhao@163.com。
引文格式:陈梦义,徐梅,钱路,等. DNA条形码试剂盒检测技术在墨天牛鉴定中的应用[J]. 南京林业大学学报:自然科学版,2015,39(6):7-11.
更新日期/Last Update: 2015-11-30