[1]赵晨光,李燕杰,张平冬*,等.胡杨未减数2n花粉形成的细胞学机制[J].南京林业大学学报(自然科学版),2015,39(06):040-44.[doi:10.3969/j.issn.1000-2006.2015.06.008]
 ZHAO Chenguang,LI Yanjie,ZHANG Pingdong*,et al.Cytological mechanism of unreduced 2n pollen formation in Populus euphratica[J].Journal of Nanjing Forestry University(Natural Science Edition),2015,39(06):040-44.[doi:10.3969/j.issn.1000-2006.2015.06.008]
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胡杨未减数2n花粉形成的细胞学机制
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
39
期数:
2015年06期
页码:
040-44
栏目:
研究论文
出版日期:
2015-11-30

文章信息/Info

Title:
Cytological mechanism of unreduced 2n pollen formation in Populus euphratica
文章编号:
1000-2006(2015)06-0040-05
作者:
赵晨光李燕杰张平冬*康向阳
林木育种国家工程实验室,林木花卉遗传育种教育部重点实验室,北京林业大学,北京 100083
Author(s):
ZHAO Chenguang LI Yanjie ZHANG Pingdong* KANG Xiangyang
National Engineering Laboratory for Tree Breeding, Key Laboratory for Genetic and Breeding in Forest Trees and Ornamental Plants, Ministry of Education,Beijing Forestry University, Beijing 100083, China
关键词:
胡杨 花粉母细胞 减数分裂 微管骨架 胞质分裂 未减数2n花粉
Keywords:
Populus euphratica pollen mother cell meiosis microtubule cytoskeleton cytokinesis unreduced 2n pollen
分类号:
Q343; S792.11
DOI:
10.3969/j.issn.1000-2006.2015.06.008
文献标志码:
A
摘要:
为了研究花粉母细胞减数分裂染色体的异常行为以及胞质分裂过程中微管骨架变化,利用液氮冷冻压片法和间接免疫荧光结合DAPI染色法,对胡杨花粉母细胞减数分裂过程中染色体行为和微管骨架进行了观察。结果表明:①胡杨花粉母细胞减数分裂前期Ⅰ同源染色体联会结合松散,在终变期可以观察到单价体; 中期Ⅱ存在平行纺锤体、三极纺锤体以及融合纺锤体; 后期Ⅰ以及后期Ⅱ可观察到落后染色体。②三极纺锤体以及融合纺锤体与未减数2n花粉比率呈正相关,说明三极纺锤体和融合纺锤体会导致FDR(first division restitution)型2n花粉的产生。③前期Ⅱ存在胞质分裂提前现象,可导致SDR(second division restitution)型2n花粉形成。④后期Ⅱ纺锤体微管缺失致使姊妹染色体单体分离后不移向两极,保留在同一子核内,这可能是形成SDR型2n花粉的另一种机制。
Abstract:
In order to analyze abnormal behaviors of chromosomes during meiosis and abnormal organization of microtubule during meiosis and cytokinesis, and find a new method to illustrate unreduced 2n pollen formation, indirect immunofluorescence microscopy with DAPI staining was conducted to observe the microtubule cytoskeleton pattern and chromosomes behavior during meiosis and cytokinesis in Populus euphratica. The results indicated that: ① During the prophase stage of meiosis, the paired homologous chromosomes combined, loosely, with univalent at diakinesis. Parallel spindle, tripolar spindle and fused spindle could also been found, at metaphase Ⅱ Lagging chromosomes could be observed at anaphaseⅠand anaphaseⅡ. ② Positive correlations between frequency of 2n pollen and rate of tripolar spindle and fused spindle were found, which suggested that tripolar spindle and fused spindle could result in FDR-typed 2n pollen formation. ③ Prematurely cytokinesis was observed at prophaseⅡ, which resulted in SDR-typed 2n pollen formation in Populus euphratica. ④ Due to lack of spindle microtubule in one daughter cell at anaphaseⅡ, the chromatids do not migrated to the poles instead of retaining in the daughter cell and forming a SDR-typed 2n pollen.

参考文献/References:

[1] Veilleux R E, McHale N A, Lauer F I. 2n gametes in diploid Solanum: frequency and types of spindle abnormalities[J]. Canadian Journal of Genetics and Cytology, 1982, 24(3): 301-314.
[2] Dewitte A, Eeckhaut T, Van Huylenbroeck J, et al. Meiotic aberrations during 2n pollen formation in Begonia[J]. Heredity, 2010, 104(2): 215-223.
[3] Zhang Z H, Kang X Y. Cytological characteristics of numerically unreduced pollen production in Populus tomentosa Carr.[J]. Euphytica, 2010, 173(2): 151-159.
[4] 张平冬,康向阳,韩锦. 胡杨嫁接繁殖研究初报[J].干旱区资源与环境, 2005, 19(4):197-200. Zhang P D, Kang X Y, Han J. Preliminary studies on grafting propagation of Populus euphratica[J]. Journal of Arid Land Resources and Environment, 2005, 19(4): 197-2000.
[5] 李文钿,朱彤.胡杨花粉和胚囊发育[J]. 林业科学研究, 1988, 1(2):132-137. Li W T, Zhu T. Development of pollen and embryosac in Populus euphratca Oliv.[J]. Forest Research, 1998, 1(2):132-137.
[6] 张平冬,康向阳. 胡杨小孢子发生及微管骨架变化与异常研究[J].西北植物学报, 2013, 33(11):2166-2171. Zhang P D, Kang X Y. Organization of microtubule and its abnormities during microsporogenesis in Populus euphratica[J]. Acta Bot Boreal, 2013, 33(11):2166-2171.
[7] Orjeda G, Freyre R, Iwanaga M. Production of 2n pollen in diploid Ipomoea trifida, a putative wildancestor of sweet potato[J]. J Hered, 1990, 81(6): 462-467.
[8] Islam A K M R, Shepherd K W. Meiotic restitution in wheat-barley hybrids[J]. Chromosoma, 1980, 79: 363-372.
[9] Bretagnolle F, Thompson J D. Gametes with the somatic chromosome number: mechanisms of their formation and role in the evolution of autopolyploid plants[J]. New Phytol, 1995, 129(1):1-22.
[10] Ramsey J, Schemske D W. Pathway, mechanisms, and rates of polyploid formation in flowering plants[J]. Annu Rev Ecol Syst, 1998, 29:467-501.
[11] Mok D W S, Peloquin S J. Three mechanisms of 2n pollen formation in diploid potatoes[J]. Can J Genet Cytol, 1975, 17(2):217-225.
[12] d'Erfurth I, Jolivet S, Froger N, et al. Mutations in AtPS1(Arabidopsis thaliana parallel spindle 1)lead to the production of diploid pollen grains[J]. PLoS Genet, 2008, 4(11):1000274.
[13] Ramanna M S. A re-examination of the mechanisms of 2n gamete formation in potato and its implications for breeding[J].Euphytica, 1979, 28(3):537-561.
[14] Zhang J F,Wei Z, Li D, et al. Using SSR markers to study the mechanism of 2n pollen formation in Populus × euramericana(Dode)Guinier and P. × popularis[J]. Ann For Sci, 2009, 66(56):506.
[15] 康向阳.毛白杨2n花粉的发生机制[J]. 北京林业大学学报,2002,24(5):67-70. Kang X Y.Mechanism of 2n pollen occurring in Chinese white poplar[J]. Journal of Beijing Forestry University, 2002, 24(5):67-70.
[16] 鲁敏. 响叶杨三倍体和四倍体诱导技术研究[D].北京: 北京林业大学, 2013. Lu M. Techniques of triploid and teraploid induction in Populus adenopoda Maxim.[D]. Beijing:Beijing Forestry University, 2013.
[17] 王君. 青杨派树种多倍体诱导技术研究[D]. 北京: 北京林业大学, 2009. Wang J. Techniqnes of polyphoid induction in Populus spp.(Section Tacamahaca)[D]. Beijing:Beijing Forestry University, 2009.
[18] Manzos A M. Fast growing form of Populus balsamifera obtained by pollinating female flowers with fractionated pollen of the same species[J].DoklAkad NaukS SSR, 1960, 130(2): 433-435.
[19] Seitz F W. The occurrence of triploids after self-pollination of anomalous androgynous flowers of a gray poplar[J]. Z Forstgenet, 1954, 3(1): 1-6.

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备注/Memo

备注/Memo:
收稿日期:2014-01-26 修回日期:2014-09-08
基金项目:国家自然科学基金项目(31370659)
第一作者:赵晨光,硕士生。*通信作者:张平冬,副教授,博士。E-mail: zhangpd@bjfu.edu.cn。
引文格式:赵晨光,李燕杰,张平冬,等. 胡杨未减数2n花粉形成的细胞学机制[J]. 南京林业大学学报:自然科学版,2015,39(6):40-44.
更新日期/Last Update: 2015-11-30