[1]鲁 路,张景波,陈金慧,等.比拉底白刺苗期抗旱性分析[J].南京林业大学学报(自然科学版),2015,39(06):051-55.[doi:10.3969/j.issn.1000-2006.2015.05.010]
 LU Lu,ZHANG Jingbo,CHEN Jinhui,et al.Drought resistance analysis of Nitraria billardieri seedlings[J].Journal of Nanjing Forestry University(Natural Science Edition),2015,39(06):051-55.[doi:10.3969/j.issn.1000-2006.2015.05.010]
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比拉底白刺苗期抗旱性分析
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
39
期数:
2015年06期
页码:
051-55
栏目:
研究论文
出版日期:
2015-11-30

文章信息/Info

Title:
Drought resistance analysis of Nitraria billardieri seedlings
文章编号:
1000-2006(2015)06-0051-05
作者:
鲁 路1张景波2陈金慧1周艳威1成铁龙1*
1.林木遗传与生物技术省部共建教育部重点实验室,南京林业大学林学院,江苏 南京 210037;
2.中国林业科学研究院沙漠林业实验中心,内蒙古 磴口 015200
Author(s):
LU Lu1 ZHANG Jingbo2 CHEN Jinhui1 ZHOU Yanwei1 CHENG Tielong1*
1.Key Laboratory of Forest Genetics and Biotechnology, Ministry of Education, College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
2.The Experimental Center for Desert Forestry, Chinese Academy of Forestry, Dengkou 015200, China
关键词:
比拉底白刺苗 生理指标测定 抗旱性分析
Keywords:
Nitraria billardieri seedling physiological indexes determination drought resistance analysis
分类号:
S718; Q945.78
DOI:
10.3969/j.issn.1000-2006.2015.05.010
文献标志码:
A
摘要:
为研究澳大利亚荒漠植物比拉底白刺的抗旱性,以比拉底白刺幼苗作为研究材料,在100 mmol/L甘露醇溶液模拟干旱的环境下,观察了植物形态变化,测定了植物的相对含水量(relative water content,RWC)、过氧化物酶活性(peroxidase,POD)、丙二醛(malondiadehyde,MDA)以及游离脯氨酸(proline,Pro)含量的动态变化。结果表明,干旱处理过程中,比拉底白刺叶片从基部往上逐渐萎蔫变黄,推测其通过保护顶芽的生命力以适应干旱环境; 相对含水量前期相对稳定,后逐渐下降; POD活性先缓慢升高,后快速上升; 游离Pro浓度总体呈上升趋势; MDA含量先升后降。差异显著性分析表明,与对照相比,POD和游离Pro的变化趋势与对照组相比差异显著,说明二者与比拉底白刺的抗旱性具有相关性,通过减少质膜过氧化保护细胞完整性; MDA含量变化不明显,可能由于POD和游离脯氨酸的作用使得白刺细胞的膜脂系统未遭受活性氧自由基的强烈攻击。
Abstract:
The drought resistance research of Australia desert plants Nitraria billardieri was focused by observing morphological changes and analyzing variation trends of relative water content(RWC), enzymatic activity of peroxidase(POD), malondiadehyde(MDA), and proline(Pro)under drought stress simulated by 100 mmol/L mannitol solution. The results showed that the leaves turned yellow under drought stress and withered gradually from base to top, presumably by protecting the vitality of buds to adapt the arid environments. The relative water content kept relatively stable at early stage and then decreased gradually. The POD activity increased slowly at first and then increased rapidly. Free Pro concentration had been rising during the treatment. MDA content increased at first and then decreased. According to the statistical analysis, both POD and free Pro increased obviously compared with control group. They reduced plasma membrane peroxidation and kept the cell integrity which showed drought resistance of Nitraria billardieri. MDA concentration did not change significantly, this indicated that cytoplasmic membrane system was protected and did not seriously suffer from reactive oxygen free radical attacking under drought stress probably caused by increased concentration of POD and free Pro.

参考文献/References:

[1] 左凤月,郝秀芬,陈占峰,等.小果白刺和泡果白刺的耐盐性[J].天津农学院学报,2013,20(2):11-14. Zuo F Y, Hao X F, Chen Z F, et al. Salt tolerance of Nitraria sibirica Pall. and Nitraria roborowskii Kom[J]. Journal of Tianjin Agricultural College, 2013, 20(2):11-14.
[2] 张桂玲.等渗透势盐分和水分胁迫对白刺幼苗生长及离子吸收分配的影响[J].干旱地区农业研究,2013,31(4):114-118. Zhang G L. Effects of iso-osmotic salt and water stresses on growth and ionic absorption and distribution in Nitraria sibirica seedlings[J]. Agricultural Research in the Arid Areas, 2013, 31(4):114-118.
[3] 芦静,曹俊梅,周安定,等.PEG处理下新疆冬小麦品种幼苗期生理指标的抗旱性研究[J].新疆农业科学,2014,51(3):393-402. Lu J,Cao J M,Zhou A D, et al. Study on drought-resistance physiological mechanism under PEG water-stress of winter wheat varieties at seedling stage in Xinjiang[J]. Xinjiang Agricultural Sciences, 2014, 51(3):393-402.
[4] 高瞑,李毅,种培芳.等.渗透胁迫下不同地理种源白刺的生理响应[J].草业学报,2011,20(3):99.
[5] 王秀娟,杨会青.盐碱胁迫下西伯利亚白刺的渗透调节物质的变化[J].热带农业科学,2010,30(2):34-36. Wang X J, Yang H Q. Changes of osmosis-regulating substance of Nitraria sibirica Pall. under salt-alkali stress in Daqing[J]. Chinese Journal of Tropical Agriculture, 2010, 30(2):34-36.
[6] 潘晓玲,沈观冕,陈鹏.白刺属植物的分类学及系统学研究[J].云南植物研究,1999,21(3):287-295. Pan X L, Shen G M, Chen P. A preliminary research on taxonomy and systematics of genus Nitraria[J]. Acta Botanica Yunnanica, 1999, 21(3):287-295.
[7] 李毅,屈建军,张伟民,等.白刺对干旱胁迫的生理生化反应[J].中国农学通报,2012,28(01):24-29. Li Y, Qu J J, Zhang W M, et al. Physiological and biochemical responses of Nitraria L. to drought stress[J]. Chinese Agricultural Science Bulletin, 2012, 28(01):24-29.
[8] 杨钦周.白刺属植物的川西新记录和澳大利亚间断分布途径的探讨[J].山地学报,2006,24(02):137-143.
[9] Shen M Y,Zuanazzi J A, Kan C, et al. Alkaloids from Nitraria billardieri[J]. Natural Product Letters, 1995, 6(2): 119-125.
[10] Noble J C. The effects of emus(Dromaius novaehollandiae Latham)on the distribution of the nitre bush(Nitraria billardieri DC.)[J]. The Journal of Ecology, 1975,63(3): 979-984.
[11] Noble J C, Whalley R D B. The biology and autecology of Nitraria L. in Australia. II: Seed germination, seedling establishment and response to salinity[J]. Australian Journal of Ecology, 1978, 3(2): 165-177.
[12] An Y Y, Zhang M X, Liu G B, et al. Proline accumulation in leaves of Periploca sepium via both biosynthesis up-regulation and transport during recovery from severe drought[J]. PloS One, 2013, 8(7):e69942.
[13] 薛彦斌,秦华,张科.3种藤本地被植物抗旱性比较[J].中国农学通报,2010,26(9):239-243. Xiu Y B, Qin H, Zhang K. Comparative study of drought-resistance on three of ground cover plants[J]. Chinese Agricultural Science Bulletin, 2010, 26(9):239-243.
[14] 于金慧,柏明娥,方伟,等.干旱胁迫对4 种灌木生理生化特性的影响[J].浙江林学院学报,2009,26(4):485-489. Yu J H, Bo M E, Fang W, et al. Physiological and biochemical substances of four shrubs with drought stress[J]. Journal of Zhejiang Forestry College, 2009, 26(4):485-489.
[15] 路文静,李奕松.植物生理学实验教程[M].北京:中国林业出版社,2012:100-110. Lu W J, Li Y S. Plant physiology experiment tutorial[M]. Beijing: China Forestry Publishing, 2012:100-110.
[16] 崔豫川,张文辉,李志萍.干旱和复水对栓皮栎幼苗生长和生理特性的影响[J].林业科学,2014,50(7):66-73. Cui Y C, Zhang W H, Li Z P. Effects of drought stress and rewatering on growth and physiological characteristics of Quercus variabilis Seedlings[J]. Scientia Silvae Sinicae, 2014, 50(7):66-73.
[17] Engelbrecht B M J, Kursar T A. Comparative drought-resistance of seedlings of 28 species of co-occurring tropical woody plants[J]. Oecologia, 2003, 136(3):383-393.
[18] 李正理.旱生植物的形态和结构[J].生物学通报, 1981(4):9-12. Li Z L. Morphology and structure of xeric plants[J]. Bulletin of Biology, 1984(4):9-12.
[19] 孙永玉,李昆,崔永忠,等.干热河谷柠檬桉苗期抗旱生理特性[J].东北林业大学学报,2009,37(2):1-2. Sun Y Y, Li K, Cui Y Z, et al. Drought resistant physiological characteristics of Eucalyptus maculate seedlings in dry-hot valley[J]. Journal of Northeast Forestry University, 2009, 37(2):1-2.
[20] Farooq M, Hussain M, Han F. Plant responses to drought stress[M]. New York: Dordrecht London, 2012.
[21] Fu J,Huang B. Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress[J]. Environmental and Experimental Botany, 2001, 45(2):105-114.
[22] Liu H, Zhang Y H, Yin H, et al. Alginate oligosaccharides enhanced Triticum aestivum L. tolerance to drought stress[J]. Plant Physiology and Biochemistry, 2013, 62:33-40.
[23] Zhu J Y, Li L, Jia Z Q. Research advances on drought resistance mechanism of plant species in arid zones of China[J]. Sciences in Cold and Arid Regions, 2011, 3(5):0448-0454.
[24] Seki M, Umezawa T, Urano K, et al. Regulatory metabolic networks in drought stress responses[J]. Current Opinion in Plant Biology, 2007, 10(3):296-302.
[25] 赵丽英,邓西平,山仑.活性氧清除系统对干旱胁迫的响应机制[J].西北植物学报,2005,25(2):413-418. Zhao L Y, Deng X P, Shan L. The response mechanism of active oxygen species removing system to drought stress[J]. Acta Botanica Boreali-Occidentalia Sinica, 2005, 25(2):413-418.
[26] 孙军伟,孙世贤,杨国航,等.玉米苗期抗旱性鉴定指标的研究[J].华北农学报, 2008,23(S1):114-117. Sun J W, Sun S X, Yang G H, et al. Study on identification dexes of the drought resistance at Maize seedling stage[J]. Acta Agriculturae Boreali-Sinica, 2008, 23(S1): 114-117.
[27] Nanjo T, Kobayashi M, Yoshiba Y, et al. Biological functions of proline in morphogenesis and osmotolerance revealed in antisense transgenic Arabidopsis thaliana [J]. The Plant Journal, 1999, 18(2):185-193.
[28] Sharma S, Villamor J G, Verslues P E. Essential role of tissue-specific proline synthesis and catabolism in growth and redox balance at low water potential[J]. Plant Physiology, 2011, 157(1):292-304.
[29] Ashraf M, Foolad M R. Roles of glycine betaine and proline in improving plant abiotic stress resistance[J]. Environmental and Experimental Botany, 2007, 59(2):206-216.
[30] 薛瑞,周广奇,胡新文,等.柱花草种质抗旱性综合评价[J].中国农学通报,2009,25(11):224-233. Xue R, Zhou G Q, Hu X W, et al. Comprehensive evaluation of drought resistance in Stylosanthes germplasms[J]. Chinese Agricultural Science Bulletin, 2009, 25(11):224-233.

备注/Memo

备注/Memo:
收稿日期:2014-06-01 修回日期:2014-09-11
基金项目:国家自然科学基金项目(31270707); 中国林业科学研究院基本科研业务费专项项目(CAFYBB2011001); 国家林业局“948”引进项目(2011-4-27)
第一作者:鲁路,硕士生。*通信作者:成铁龙,高级工程师。E-mail:ctielong@126.com。
引文格式:鲁路,张景波,陈金慧,等. 比拉底白刺苗期抗旱性分析[J]. 南京林业大学学报:自然科学版,2015,39(6):51-55.
更新日期/Last Update: 2015-11-30