[1]黄 鹏,毛 霞,韩 歌,等.雌雄异型异熟青钱柳花发育过程中养分的动态变化[J].南京林业大学学报(自然科学版),2018,42(05):001-9.[doi:10.3969/j.issn.1000-2006.201802018]
 HUANG Peng,MAO Xia,HAN Ge,et al.Dynamic changes of nutrients during flora development in heterodichogamous Cyclocarya paliurus[J].Journal of Nanjing Forestry University(Natural Science Edition),2018,42(05):001-9.[doi:10.3969/j.issn.1000-2006.201802018]
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雌雄异型异熟青钱柳花发育过程中养分的动态变化
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
42
期数:
2018年05期
页码:
001-9
栏目:
研究论文
出版日期:
2018-09-15

文章信息/Info

Title:
Dynamic changes of nutrients during flora development in heterodichogamous Cyclocarya paliurus
文章编号:
1000-2006(2018)05-0001-09
作者:
黄 鹏毛 霞韩 歌陈小玲洑香香*
南京林业大学,南方现代林业协同创新中心,南京林业大学林学院,江苏 南京 210037
Author(s):
HUANG Peng MAO Xia HAN Ge CHEN Xiaolin FU Xiangxiang*
Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China
关键词:
青钱柳 雌雄异型异熟 花芽发育 可溶性养分 淀粉
Keywords:
Cyclocarya paliurus (Batal.)Iljinskaja heterodichogamous floral development soluble nutrient starch
分类号:
S718; Q945.61
DOI:
10.3969/j.issn.1000-2006.201802018
文献标志码:
A
摘要:
【目的】青钱柳集药用、材用、观赏于一身,是典型的雌雄异型异熟植物,通过研究不同交配类型雌雄花芽(序)发育过程中花芽(序)和周围组织内养分的动态变化,以阐述其雌雄异熟的养分调控机理。【方法】在花发育期间,从青钱柳林分中随机选取两种交配类型(雄先型(简称PA),雌先型(简称PG)),观测花芽(序)(雄花♂,雌花♀)形态变化并测量花序长度; 同时测定两种交配类型花芽(序)及其周围组织(雄花枝条(记为♂Z),雌花枝条(记为♀Z)和雌花叶片(记为♀Y))内的淀粉、可溶性糖和可溶性蛋白含量。【结果】①淀粉:花芽萌动前,花和枝条上均积累了大量淀粉,并随着花的发育呈持续下降的趋势; ♂Z的含量显著高于♀Z,PA的♂Z上的含量高于PG的♂Z上的。②可溶性糖:萌动前♂Z积累了大量的可溶性糖,在发育过程中其含量呈现下降、上升并趋于稳定的动态过程,而♂内的含量出现波动但相对稳定; 在♀及其周围组织均呈先上升后下降的趋势,在花序伸长生长结束时达到峰值。③可溶性蛋白质:萌动前PA的♂比PG积累了更多的可溶性蛋白,在两种交配类型♂上可溶性蛋白含量均随着花的发育逐渐下降,♂Z内的可溶性蛋白含量在发育过程中相对稳定; 可溶性蛋白在♀花序开始伸长生长时达到高峰,而♀Y中的含量呈持续下降,♀Z上可溶性蛋白的变化模式在两种交配类型上存在差异。【结论】雌雄花发育所需可溶性糖主要来自自身和枝条内贮藏的可溶性糖和淀粉(水解产物),但PA的♂Z内贮藏的可溶性糖含量远高于PG的; 雄花发育所需可溶性蛋白来自自身或光合产物的输入,雌花则主要来自光合产物的输入。淀粉和可溶性养分的动态变化在不同交配类型雌雄花上均存在时序差异,但与花序的发育进程一致。
Abstract:
【Objective】Cyclocarya paliurus is a typical heterodichogamous species. To demonstrate nutrient the regulatory mechanism of heterodichogamous characteristics of flowering in C. paliurus, dynamic changing patterns of nutrients in the flowers and surrounding tissues during various developmental stages of the floral were monitored. 【Method】 In two mating types(protandry, PA and protogyny, PG)of C. paliurus selected from a plantation, changes in morphology and length of male and female inflorescences(male, ♂; female, ♀)were observed and measured; also, dynamic changes in starch, soluble sugar, and soluble protein content in the flowers and surrounding tissues(branch surrounding male bud,♂Z; branch and leaves surrounding female bud,♀Z and♀Y)during the process of floral development were measured. 【Result】 ① Starch: much starch was found in the flower buds and branches before initiation, and the contents declined with the process of inflorescence development. The content of starch in♂Z was significantly higher than in♀Z, and the content in♂Z of PA was higher than that of PG. ②Soluble sugar: large amounts of soluble sugar were found in♂Z before bud initiation and changing patterns of soluble sugar showed a dynamic pattern of “declining to increasing, then a tendency to stabilize.” The content in the♂fluctuated with a little change, whereas the tendency of “rising to declining” and the maximum in the end of elongation growth of the ♀ occurred in the flower and surrounding tissues. ③ Soluble protein: more soluble protein was accumulated in the♂of PA than PG before bud initiation, and the content decreased gradually with floral development; whereas the content in♂Z changed a little. Soluble protein in the♀reached a peak before elongation growth of the inflorescence; whereas changing patterns of the content in♀Y of PA were observed. However, changing patterns of the soluble protein content in♀Z were different between PA and PG. 【Conclusion】Soluble sugar for floral development was from soluble sugar and hydrolysis products of starch stored in flowers and surrounding the branch, whereas soluble sugar stored in♂Z of PA was much higher than that of PG. Soluble protein stored in the♂or photosynthetic products input provided the needs for male flower development, whereas photosynthetic products were the only source for female flower development. Although changing patterns of starch and soluble nutrients are different in stages and sequences of male and female flowers between PA and PG, they were synchronized precisely with the process of floral development.

参考文献/References:

[1] 毛霞, 刘晶晶, 李晓春, 等. 雌雄异型异熟青钱柳幼龄林开花习性及交配系统分析[J]. 南京林业大学学报(自然科学版), 2016, 40(6): 47-55. DOI: 10.3969/j.issn.1000-2006.2016.06.008.
MAO X, LIU J J, LI X C, et al. Flowering biological characteristics and mating system in immature plantations of heterodichogamous Cyclocarya paliurus[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2016, 40(6): 47-55.
[2] 谢明勇, 谢建华. 青钱柳研究进展[J]. 食品与生物技术学报, 2008, 27(1): 113-121. DOI: 10.3321/j.issn:1673-1689.2008.01.021.
XIE M Y, XIE J H. Review about the research on Cyclocarya paliurus(Batal.)Iljinskaja [J]. Journal of Food Science and Biotechnology, 2008, 27(1): 113-121.
[3] 洑香香, 冯岚, 方升佐, 等. 青钱柳开花习性及雄花发育的解剖学观察[J]. 南京林业大学学报(自然科学版), 2010, 34(3): 67-71. DOI: 10.3969/j.issn.1000-2006.2010.03.014.
FU X X, FENG L, FANG S Z, et al. Observation on flowering habits and anatomy of stamen development in Cyclocarya paliurus [J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2010, 34(3): 67-71.
[4] 洑香香, 冯岚, 尚旭岚, 等. 青钱柳雌、雄花芽分化进程的形态解剖特征观察[J]. 南京林业大学学报(自然科学版), 2011, 35(6): 17-22. DOI: 10.3969/j.jssn.1000-2006.2011.06.004.
FU X X, FENG L, SHANG X L, et al. Observation of morphological and anatomical characters on staminate and pistillate flower differentiation in Cyclocarya paliurus [J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2011, 35(6):17-22.
[5] FUKUHARA T, TOKUMARU S. Inflorescence dimorphism, heterodichogamy and thrips pollination in Platycarya strobilacea(Juglandaceae)[J]. Annals of Botany, 2014, 113(3): 467-476. DOI: 10.1093/aob/mct278.
[6] FIELD D L, BARRETT S C. Disassortative mating and the maintenance of sexual polymorphism in painted maple[J]. Molecular Ecology, 2012, 21(15): 3640-3643. DOI: 10.1111/j.1365-294X.2012.05643.x.
[7] BAI W N, ZENG Y F, ZHANG D Y. Mating patterns and pollen dispersal in a heterodichogamous tree, Juglans mandshurica (Juglandaceae)[J]. New Phytologist, 2007, 176(3): 699. DOI: 10.1111/j.1469-8137.2007.02202.x.
[8] 汪晓谦, 张延龙, 牛立新, 等. 郁金香花芽分化过程中鳞茎碳水化合物和蛋白质含量的变化[J]. 植物生理学报, 2011, 47(4): 379-384. DOI:10.13592/j.cnki.ppj.2011.04.006
WANG X Q, ZHANG Y L, NIU L X, et al. Changes of carbohydrate and protein contents in bulbs of Tulipa gesneriana L. during flower bud differentiation [J]. Plant Physiology Communications, 2011, 47(4): 379-384.
[9] 曲波, 张微, 陈旭辉, 等. 植物花芽分化研究进展[J]. 中国农学通报, 2010, 26(24): 109-114.
QU B, ZHANG W, CHEN X H, et al. Research progress of flower bud differentiation mechanism of plant [J]. Research Progress of Flower Bud Differentiation Mechanism of Plant, 2010, 26(24): 109-114.
[10] GLEISER G, VERDU M, SEGARRA-MORAGUES J G, et al. Disassortative mating, sexual specialization, and the evolution of gender dimorphism in heterodichogamous Acer opalus [J]. Evolution, 2008, 62(7): 1676-1688. DOI: 10.1111/j.1558-5646.2008.00394.x.
[11] KIMURA M K, GOTO S, SUYAMA Y, et al. Morph-specific mating patterns in a low-density population of a heterodichogamous tree, Juglans ailantifolia [J]. Plant Ecology, 2012, 213(9):1477-1487. DOI: 10.1007/s11258-012-0105-6.
[12] WATANABE S, NOMA N, NISHIDA T. Flowering phenology and mating success of the heterodichogamous tree Machilus thunbergii Sieb. et Zucc(Lauraceae)[J]. Plant Species Biology, 2016, 31(1):29-37. DOI: 10.1111/1442-1984.12078.
[13] SHIBATA M, KIKUCHI S, TANAKA H, et al. Effects of population density, sex morph, and tree size on reproduction in a heterodichogamous maple, Acer mono, in a temperate forest of Japan[J]. Ecological Research, 2009, 24(1):1-9. DOI: 10.1007/s11284-008-0474-4.
[14] 李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社, 2000: 184-205.
LI H S.Principles and techniques of plant physiology and biochemistry experiments[M]. Beijing: Higher Education Press, 2000: 184-205.
[15] 钟晓红, 罗先实, 陈爱华. 奈李花芽分化与体内主要代谢产物含量的关系[J]. 湖南农业大学学报, 1999, 25(1): 31-35. DOI:10.13331/j.cnki.jhau.1999.01.008.
ZHONG X H, LUO X S, CHEN A H. A study on nai plum's flower bud differentiation and its major content of metabolic production [J]. Journal of Hunan Agricultural University, 1999, 25(1): 31-35.
[16] LA ROSA R,RALLO L, HAYA F. Olive floral bud growth and starch content during winter rest and spring bud break[J]. HortScience,2000,35(7): 1227-1234.
[17] 李兴军, 李苎玉, 汪国云, 等. 杨梅花芽孕育期间叶片酸性蔗糖酶活性及糖类含量的变化[J]. 四川农业大学学报, 2000, 18(2): 164-166. DOI:10.16036/j. issn.1000-2650.2000.02.018.
LI X J, LI Z Y, WANG G Y, et al. Changes of acid invertase activity and correlated sugar level in bayberry leaves during flower bud initiation stage [J]. Journal of Sichuan Agricultural University, 2000, 18(2): 164-166.
[18] 樊卫国, 刘国琴, 安华明. 刺梨花芽分化期芽中内源激素和碳氮营养的含量动态[J]. 果树学报, 2003, 20(l): 40-43. DOI:10.13925/j.cnki.gsxb.2003.01.010.
FAN W G, LIU G Q, AN H M. Study on the changes of endogenous hormones, carbohydrate and nitrogen nutrition at the flower bud differentiation stage of Rosa roxburghii[J]. Journal of Fruit Science, 2003, 20(l): 40-43.
[19] 胡玉玲, 姚小华, 张山, 等. 油茶花器官形成过程花芽与叶片生理生化变化[J]. 江西农业大学学报, 2016, 38(3): 412-417. DOI: 10.13836 / j.jjau.2016059.
HU Y L, YAO X H, ZHANG S, et al. Physiological and biochemical change in flower buds and leaves during the formation of floral organs of Camellia oleifera [J]. Acta Agriculturae Universitatis Jiangxiensis, 2016, 38(3): 412-417.
[20] 涂淑萍, 穆鼎, 刘春. 不同百合品种花芽分化期的生理生化变化[J]. 中国农学通报, 2005, 21(7): 207-209. DOI: 10.3969/j.issn.1000-6850.2005.07.063.
TU S P, MU D, LIU C. The physiological and biochemical changes during flower bud differentiation of different lily cultivars[J]. Chinese Agricultural Science Bulletin, 2005, 21(7): 207-209.
[21] 罗羽洧, 解卫华, 马凯. 无花果花芽分化与营养物质含量的关系[J]. 江西农业大学学报, 2008, 30(1): 40-43. DOI:10.3969/j.issn.1000-2286.2008.01.009.
LUO Y W, XIE W H, MA K. Correlation between nutrition material and flower bud differentiation of Ficus caraca L. [J]. Acta Agriculturae Universitatis Jiangxiensis, 2008, 30(1): 40-43.

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备注/Memo

备注/Memo:
收稿日期:2018-02-13 修回日期:2018-06-28 基金项目:国家自然科学基金项目(31470637); 江苏高校优势学科建设工程资助项目(PAPD) 第一作者:黄鹏(1024962457@qq.com)。*通信作者:洑香香(xxfu@njfu.edu.cn),教授。
更新日期/Last Update: 2018-09-15