[1]吴 凡,吴小芹*.细胞自噬对松材线虫繁殖存活的影响及线虫与松树互作早期自噬相关基因的表达[J].南京林业大学学报(自然科学版),2018,42(05):059-64.[doi:10.3969/j.issn.1000-2006.201712025]
 WU Fan,WU Xiaoqin*.The role of autophagy of pine wood nematode Bursaphelenchus xylophilus in the growth and development and infecting pine trees at early time[J].Journal of Nanjing Forestry University(Natural Science Edition),2018,42(05):059-64.[doi:10.3969/j.issn.1000-2006.201712025]
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细胞自噬对松材线虫繁殖存活的影响及线虫与松树互作早期自噬相关基因的表达
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
42
期数:
2018年05期
页码:
059-64
栏目:
研究论文
出版日期:
2018-09-15

文章信息/Info

Title:
The role of autophagy of pine wood nematode Bursaphelenchus xylophilus in the growth and development and infecting pine trees at early time
文章编号:
1000-2006(2018)05-0059-06
作者:
吴 凡吴小芹*
南京林业大学,南方现代林业协同创新中心,南京林业大学林学院,江苏省有害生物入侵预防与控制重点实验室,江苏 南京 210037
Author(s):
WU Fan WU Xiaoqin*
Co-Innovation Center for the Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, College of Forestry, Nanjing Forestry University, Nanjing 210037, China
关键词:
松材线虫 细胞自噬 自噬诱导剂 雷帕霉素 外源诱导 基因表达
Keywords:
Bursaphelenchus xylophilus autophagy autophagy inducer Rapamycin exogenous induction gene expression
分类号:
S763
DOI:
10.3969/j.issn.1000-2006.201712025
文献标志码:
A
摘要:
【目的】细胞自噬在真核生物细胞应对不良环境中具有重要作用。探讨细胞自噬对松材线虫取食繁殖的影响,测定松材线虫与松树互作过程中松材线虫BxATG1和BxATG8两个自噬相关基因的表达。【方法】在前期研究发现松材线虫存在细胞自噬现象,并克隆了松材线虫BxATG1和BxATG8两个自噬相关基因的基础上,采用外源自噬诱导剂探讨了细胞自噬对松材线虫取食繁殖的影响,测定了松材线虫与松树互作早期自噬相关基因的表达。【结果】经雷帕霉素处理后,松材线虫的自噬启动基因BxATG1和自噬标志性基因BxATG8表达量显著上调,且松材线虫的取食速率及繁殖量也显著增加; 松材线虫在饥饿状态下的存活能力明显上升,脂肪含量的堆积也略有增加。松材线虫与松树互作早期(6、12、18、24 h)的qRT-PCR结果表明,松材线虫侵染松树12 h时,细胞自噬爆发达到最高峰。【结论】外源自噬诱导后促进了松材线虫细胞自噬的发生; 细胞自噬在促进松材线虫生长发育中具有一定作用; 在松材线虫侵染松树过程中,细胞自噬有助于线虫适应松树的早期抵抗反应。
Abstract:
【Objective】Autophagy plays an important role in Eukaryotic cells adapting adversities. Nevertheless, there're few researches into autophagy and its functions in pine wood nematode(PWN), which is the pathogeny of pine wilt disease. 【Method】Our laboratory previously found that there existed autophagy in PWNs, thus, we cloned genes BxATG1 and BxATG8 of them, as the genes were related to autophagy. Based on that, this study discussed the impact of autophagy on PWNs' growth and development, as well as its roles during the interaction between PWNs and pines. 【Result】After being treated with Rapamycin, which was the autophagy inducer, the expression of BxATG1 and BxATG8 rose significantly, which demonstrated that the exogenous autophagy induction had promoted the occurrence of autophagy. Besides, the exogenous autophagy inducer notably helped with the boost in PWNs' feeding rate and reproduction. What's more, after autophagy induction, PWNs' viability under starving condition was stimulated obviously, and the accumulation of fats also improved. These facts showed that autophagy played an important role in the growth and development of PWNs. qRT-PCR results of early interaction between PWNs and pines showed that, the occurrence of autophagy reached its climax after infecting the pine seedlings for 12 hours, which implied that, during the course of PWNs infecting the pines, autophagy was helpful for PWNs to go through the early stage of plant's resistance response.【Conclusion】Induction of autophagy promoted the occurrence of PWN's autophagy, and autophagy played an important role in promoting the growth and development of Bursaphelenchus xylophilus. In the process of pine wood nematode infestation, autophagy can help the nematode adapt to the early resistance of pine.

参考文献/References:

[1] YANG Z, KLIONSKY D J. Eaten alive: a history of macroautophagy[J]. Nat Cell Biol,2010, 12(5):814-822. DOI:10.1038/ncb0910-814.
[2] MIZUSHIMA N. Autophagy: process and function[J]. Genes Dev,2007, 27(14):2861-2873. DOI:10.1101/gad.1599207.
[3] KANG C, YOU Y, AVERY L. Dual roles of autophagy in the survival of Caenorhabditis elegans during starvation[J]. Genes & Development, 2007, 21(17): 2161-2171. DOI:10.1101/gad.1573107.
[4] MELENDEZ A, TALLOCZY Z, SEAMAN M, et al. Autophagy genes are essential for dauer development and life-span extension in C. elegans[J]. Science, 2003, 301(5638): 1387-1391. DOI:10.1126/science.1087782.
[5] ALADZSITY I, TOTH M L, SIGMOND T, et al. Autophagy genes unc-51 and bec-1 are required for normal cell size in Caenorhabditis elegans[J]. Genetics, 2007, 177(1): 655-660. DOI: 10.1534/ genetics.107. 075762.
[6] TAKESHIGE K, BABA M, TSUBOI S, et al. Autophagy in yeast demonstrated with proteinase-deflcient mutants and conditions for its induction[J]. Cell Biol,1992, 119:301-311.
[7] JIA K, THOMAS C, AKBAR M, et al. Autophagy genes protect against Salmonella typhimurium infection and mediate insulin signaling-regulated pathogen resistance[J]. Proceedings of the National Academy of Sciences, 2009, 106(34): 14564-14569. DOI:10.1073/ pnas.0813319106.
[8] LEVINE B, YUAN J. Autophagy in cell death: an innocent convict[J]. Journal of Clinical Investigation, 2005, 115(10): 2679-2688. DOI:10.1172/JCI26390.
[9] 李明. 活性氧调节mTOR信号及其分子机制[D]. 广州:南方医科大学, 2009.
LI M. Regulation of mTOR signaling by reactive oxygen species and its mechanisms[D].Guangzhou: Southern Medical University,2009.
[10] SCHERZ-SHOUVAL R, SHVETS E, FASS E, et al. Reactive oxygen species are essential for autophagy and specifically regulate the activity of Atg4[J]. EMBO J,2007, 26:1749-1760. DOI:10.1038/sj.emboj.7601623.
[11] DENG L N. Identification of autophagy in the pine wood nematode Bursaphelenchus xylophilus and the molecular characterization and functional analysis of two novel autophagy-related genes, BxATG1 and BxATG8[J]. Molecular Science,2016,17:297. DOI:10.3390/ijms17030279.
[12] 袁为敏, 吴小芹, 叶建仁, 等. 松材线虫和拟松材线虫体内细菌的透射电镜观察及分离鉴定[J]. 微生物学报, 2011,51(8): 1071-1077. DOI:10.13343/j. cnki.wsxb.2011.08.005.
YUAN W M, WU X Q, YE J R, et al. Observation by transmission electron microscope and indentification of endophytic bacteria isolated from Bussaphelenchus xylophilus and B. mucronatus[J]. Acta Microbiological Sinica, 2011, 51(8): 1071-1077.
[13] 蒋丽丽. 雷帕霉素对UUO大鼠细胞自噬及肾小管间质纤维化的影响[D].石家庄:河北医科大学, 2014.
[14] 李佳芮, 邓玉杰, 张许平,等. 雷帕霉素对二种鳞翅目昆虫细胞自噬和凋亡的影响[J]. 昆虫知识, 2009(3): 383-388.
[15] 王晗, 米生权, 孙雅煊, 等. 壳寡糖对秀丽隐杆线虫寿命的影响[J]. 食品科学,2015,36(1):229-233.
WANG H, MI S Q, SUN Y X, et al. Effect of chitoo ligosaccharides lifespan of Caenorhabditis elegans[J]. Food Science, 2015, 36(1): 229-233.
[16] VITORIA B, ALICIA R, MAR R, et al. Evaluation of different RNA extraction methods of filamentous fungi in various food matrices[J]. LWT-Food Science and Technology, 2017,78:47-53. DOI:10.1016/j.lwt.2016.12.018.
[17] LIVAL K, SCHMITTGEN D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method[J]. Methods, 2001, 25(4): 402-408. DOI: 10.1006/meth.2001.1262.
[18] LIU X H, LU J P, ZHANG L, et al. Involvement of a Magnaporthe grisea serine/threonine kinase gene, MgATG1, in appressorium turgor and pathogenesis[J]. Eukaryotic Cell, 2007, 6(6): 997-1005. DOI:10.1128/ EC.00011-07.
[19] BRYANT B, RAIKHEL A S. Programmed autophagy in the fat body of Aedes aegypti is required to maintain egg maturation cycles[J]. PloS One, 2011,6: e25502-e25502. DOI:10.1371/journal.pone.0025502.
[20] ASAKURA M, NINOMIYA S, SUGIMOTO M, et al. Atg26-mediated pexophagy is required for host invasion by the plant pathogenic fungus Colletotrichum orbiculare[J]. The Plant Cell, 2009, 21(4): 1291-1304. DOI:10.1105/tpc.108.060996.
[21] ESKELINEN E L, MELÉNDEZ A, HALL D H, et al. Autophagy genes are essential for dauer development and life-span extension in C. elegans[J]. Science, 2003, 301(5638): 1387-1391. DOI:10.1126/science.1087782.
[22] 刘劼, 吴小芹, 应晨希,等. 松材线虫和拟松材线虫的繁殖力及其超氧自由基差异[J]. 南京林业大学学报(自然科学版), 2009, 33(3):5-8. DOI:10.3969/j.issn.1000-2006.2009.03.01.
LIU J,WU X Q,YING C X,et al.The difference of progenitive power and superoxide anion production in Bursaphelenchus xylophilus and B.mucronatus[J].Journal of Nanjing Forestry University(Natural Sciences Edition),2009,33(3):5-8.
[23] 杨宝君. 松材线虫病致病机理的研究进展[J]. 中国森林病虫, 2002(1): 27-31.

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备注/Memo

备注/Memo:
收稿日期:2017-12-11 修回日期:2018-04-27 基金项目:国家重点研发计划(2016YFC1202100); 江苏省农业科技自主创新资助项目(CX(16)1005); 江苏高校优势学科建设工程资助项目(PAPD) 第一作者:吴凡(501888347@qq.com),硕士。*通信作者:吴小芹(xqwu@njfu.edu.cn),教授。
更新日期/Last Update: 2018-09-15