[1]邓敏捷,靳冉冉,赵振利,等.硫酸二甲酯对患丛枝病毛泡桐蛋白组的影响[J].南京林业大学学报(自然科学版),2018,42(05):077-83.[doi:10.3969/j.issn.1000-2006.201801055]
 DENG Minjie,JIN Ranran,ZHAO Zhenli,et al.Effects of dimethyl sulphonate on proteome of Paulownia tomentosa seedlings infected by phytoplasma[J].Journal of Nanjing Forestry University(Natural Science Edition),2018,42(05):077-83.[doi:10.3969/j.issn.1000-2006.201801055]
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硫酸二甲酯对患丛枝病毛泡桐蛋白组的影响
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
42
期数:
2018年05期
页码:
077-83
栏目:
研究论文
出版日期:
2018-09-15

文章信息/Info

Title:
Effects of dimethyl sulphonate on proteome of Paulownia tomentosa seedlings infected by phytoplasma
文章编号:
1000-2006(2018)05-0077-07
作者:
邓敏捷靳冉冉赵振利王 哲范国强*
河南农业大学林学院,河南 郑州 450002
Author(s):
DENG Minjie JIN Ranran ZHAO Zhenli WANG Zhe FAN Guoqiang*
College of Forestry, Henan Agricultural University, Zhengzhou 450002, China
关键词:
毛泡桐 丛枝病 硫酸二甲酯(DMS) iTRAQ 蛋白质组
Keywords:
Paulownia tomentosa Paulownia witches' broom disease dimethyl sulphonate(DMS) iTRAQ proteome
分类号:
S792.43
DOI:
10.3969/j.issn.1000-2006.201801055
文献标志码:
A
摘要:
【目的】找出与泡桐丛枝病发生相关的蛋白,为研究泡桐丛枝病发病机制提供依据。【方法】利用iTRAQ技术对使用硫酸二甲酯(DMS)处理后恢复健康的毛泡桐患丛枝病苗、未经处理的毛泡桐病苗和健康苗进行蛋白质组学分析。【结果】共鉴定得到2 628个蛋白质,其中177个蛋白表达水平具有显著差异; 通过比对分析找到37个与泡桐丛枝病发生密切相关的差异表达蛋白。代谢途径分析可知,37个差异表达蛋白主要参与光合作用、植物-病原物相互作用、氧化磷酸化等途径。【结论】毛泡桐感染泡桐丛枝病过程中,与光合作用相关的蛋白受到影响,钙调蛋白(CaM)在植原体与毛泡桐的相互作用过程中发挥作用。
Abstract:
【Objective】Determine the proteins related to Paulownia broom disease(PaWB)and provide a basis for studying its pathogenesis. 【Method】We used isobaric tags for relative and absolute quantitation(iTRAQ)to analyze proteomes for PaWB-infected Paulownia tomentosa seedlings treated and untreated with dimethyl sulphonate(DMS), and healthy seedlings as the control.【Result】A total of 2 628 proteins was identified, among which 177 proteins exhibited differential expression. By comparison, 37 differentially expressed proteins were found to relate to Paulownia witches' broom disease. Pathway analysis showed that these 37 proteins were mainly involved in photosynthesis, plant-pathogen interactions, and oxidative phosphorylation pathways. 【Conclusion】During the process of PaWB occurrence, the proteins related to photosynthesis were affected. CaM was involved in the interaction between phytoplasma and Paulownia. These results have important significance for the further study of the mechanism of Paulownia witches' broom disease at the protein level.

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相似文献/References:

[1]范国强,赵改丽,翟晓巧,等.硫酸二甲酯对毛泡桐丛枝病幼苗植原体及SSR扩增位点的影响[J].南京林业大学学报(自然科学版),2012,36(03):005.[doi:10.3969/j.jssn.1000-2006.2012.03.002]
 FAN Guoqiang,ZHAO Gaili,ZHAI Xiaoqiao,et al.Effect of dimethyl sulphate on phytoplasma of Paulownia tomentosa seedling infected by witches’ broom and its DNA loci at SSR level[J].Journal of Nanjing Forestry University(Natural Science Edition),2012,36(05):005.[doi:10.3969/j.jssn.1000-2006.2012.03.002]

备注/Memo

备注/Memo:
收稿日期:2018-01-31 修回日期:2018-07-01 基金项目:河南省科技攻关计划项目(172102110050) 第一作者:邓敏捷(dengmj1980@126.com),副教授。*通信作者:范国强(zlxx64@126.com),教授。
更新日期/Last Update: 2018-09-15