[1]张 琳,郭丽丽,郭大龙,等.牡丹杂交F1代性状分离规律及混合遗传分析[J].南京林业大学学报(自然科学版),2018,42(06):051-60.[doi:10.3969/j.issn.1000-2006.201712034]
　ZHANG Lin,GUO Lili,GUO Dalong,et al.Separation analysis and mixed genetic analysis of phenotypic traits in F1 progenies of tree peony[J].Journal of Nanjing Forestry University(Natural Science Edition),2018,42(06):051-60.[doi:10.3969/j.issn.1000-2006.201712034]
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牡丹杂交F1代性状分离规律及混合遗传分析
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
42
期数:
2018年06期
页码:
051-60
栏目:
研究论文
出版日期:
2018-12-15

文章信息/Info

Title:
Separation analysis and mixed genetic analysis of phenotypic traits in F1 progenies of tree peony
作者:
张 琳1; 郭丽丽1; 郭大龙2; 侯小改1*
(1.河南科技大学农学院/牡丹学院,河南 洛阳 471023; 2.河南科技大学林学院,河南 洛阳 471023)
Author(s):
ZHANG Lin1;  GUO Lili1;  GUO Dalong2;  HOU Xiaogai1*
(1. College of Agriculture/Tree Peony, Henan University of Science and Technology, Luoyang 471023, China; 2. College of Forestry, Henan University of Science and Technology, Luoyang 471023, China)
关键词:
牡丹;  表型性状;  杂种优势;  主基因+多基因;  数量性状;  遗传分析
Keywords:
tree peony;  phenotypic trait;  heterosis;  major gene plus polygene;  quantitative character;  genetic analysis
分类号:
S685.11; S713
DOI:
10.3969/j.issn.1000-2006.201712034
文献标志码:
A
摘要:
【目的】了解牡丹主要表型性状的杂种优势和遗传特点,指导牡丹的育种实践。【方法】以牡丹品种‘凤丹’ב新日月锦’的F1群体为材料,分别于2014年和2015年调查了F1群体的株高、冠幅等20个表型性状,对这些表型性状进行了相关性分析和混合遗传分析。【结果】杂种优势分析结果显示,20个表型性状在F1群体中分离广泛,变异系数变化范围为11.03%～63.49%。除花高、花瓣宽、花瓣长、果荚高外,其余16个性状的中亲优势均达极显著水平。相关性分析结果表明,除花瓣长、果荚高外,其余18个性状间紧密相关。混合遗传分析结果显示:株高、冠幅、新枝数、果柄长、花径、开花时长、果荚直径和果荚高等8个性状均由微效多基因控制; 新枝长度、花朵数、花高、花瓣数、花瓣宽、蓇葖果数和单果荚种子质量7个性状均受两对加性-显性-上位性主基因控制; 叶长由两对等加性主基因控制; 花瓣长受两对等显性主基因控制; 叶宽、单果荚种子数量均由1对加性-显性主基因控制; 单果荚质量受两对加性-显性主基因控制。【结论】牡丹杂交F1代20个表型性状均存在杂种优势和超亲分离现象,且部分性状表现出主基因控制的遗传效应。研究为牡丹表型性状的QTL定位和分子标记辅助育种研究提供了理论基础。
Abstract:
【Objective】The understanding of heterosis and genetic analysis of main phenotypic traits will provide a feasible guidance for tree peony breeding. 【Method】In this study, twenty phenotypic traits of F1 progeny obtained from the cross of Paeonia ostii ‘Feng Dan’ and Paeonia suffruticosa ‘Xin Riyuejin’ were investigated in 2014 and 2015. In addition, correlation analysis and mixed genetic analysis were conducted on these phenotypic data. 【Result】 The results of heterosis analysis showed that 20 phenotypic traits were separated broadly among F1 plants. The variation coefficient varied from 11.03% to 63.49%. Except for the traits of the flower height, petal width, petal length and pod height, the mid-parent heterotic values of the remaining 16 traits showed a significant difference at 0.01 level. Correlation analysis showed a significant positive correlation among the remaining 18 traits, except for the traits of petal length and pod height. Mixed genetic analysis revealed that the traits of plant height, crown width, shoot number, fruit stalk length, flower diameter, flowering duration, pod diameter and pod height were controlled by minor-polygene. The traits of shoot length, flower number, flower height, petal number, petal width, follicle number per pod, and per pod seed weight were dominated by two pairs of additive-dominant-epistatic major genes. For other traits, leaf length was controlled by two pairs of equally additive major genes, petal length was controlled by two pairs of equally dominant major genes, leaf width and per pod seed number were controlled by a pair of additive-dominant major genes, and per pod weight was controlled by two pairs of additive-dominant major genes. 【Conclusion】All 20 phenotypic traits showed heterosis and transgressive segregation, and some of them were controlled by major genes. These results provide a theoretical basis for further study on quantitative trait locus(QTL)analysis and molecular marker-assisted tree peony breeding.

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备注/Memo

备注/Memo:
收稿日期:2017-12-21 修回日期:2018-08-14
基金项目:国家自然科学基金项目(31370697); 河南省自然科学基金项目(162300410079); 河南省教育厅重点项目(17A180003)
第一作者:张琳(ayzhanglin@126.com),博士生。*通信作者:侯小改(hkdhxg@126.com),教授。
更新日期/Last Update: 2018-11-30