[1]张春红,熊振豪,吴文龙*,等.黑莓果实发育成熟期木质素合成CAD酶及其基因表达[J].南京林业大学学报(自然科学版),2019,43(01):141-148.[doi:10.3969/j.issn.1000-2006.201805004]
 ZHANG Chunhong,XIONG Zhenhao,WU Wenlong*,et al.CAD enzyme activity and gene expression in connection with lignin synthesis during fruit development and ripening process of blackberry[J].Journal of Nanjing Forestry University(Natural Science Edition),2019,43(01):141-148.[doi:10.3969/j.issn.1000-2006.201805004]
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黑莓果实发育成熟期木质素合成CAD酶及其基因表达
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
43
期数:
2019年01期
页码:
141-148
栏目:
专题报道
出版日期:
2019-01-28

文章信息/Info

Title:
CAD enzyme activity and gene expression in connection with lignin synthesis during fruit development and ripening process of blackberry
文章编号:
1000-2006(2018)06-0141-08
作者:
张春红1熊振豪1吴文龙1*李维林12
1.江苏省中国科学院植物研究所,江苏 南京 210014; 2. 南京林业大学林学院,江苏 南京 210037
Author(s):
ZHANG Chunhong1 XIONG Zhenhao1 WU Wenlong1* LI Weilin12
1. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China; 2. College of Forestry, Nanjing Forestry University, Nanjing 210037, China
关键词:
黑莓 果实硬度 肉桂醇脱氢酶(CAD) 基因表达分析 果实发育
Keywords:
blackberry(Rubus spp.) fruit firmness cinnamyl alcohol dehydrogenase(CAD) gene expression analysis fruit development
分类号:
S663.2
DOI:
10.3969/j.issn.1000-2006.201805004
文献标志码:
A
摘要:
【目的】比较黑莓高硬度品种‘Arapaho’和低硬度品种‘Boysen’果实发育成熟期木质素合成肉桂醇脱氢酶(CAD)基因表达,及其与CAD酶活性及总木质素含量的关系。【方法】对不同发育时期的黑莓果肉,利用半定量RT-PCR分析18个RuCADs基因成员的表达,利用比色法测定CAD酶活性,利用滴定法测定总木质素含量。【结果】在黑莓的18个RuCAD基因成员中,有8个CADs基因成员在2个品种发育早、中期果肉中表达量较高; 其中RuCAD16仅在‘Boysen’的早、中期果肉中表达,而在‘Arapaho’中不表达。‘Arapaho’和‘Boysen’果肉CAD酶活性均在着色前即花后9 d和花后21 d时最高,着色后呈下降趋势。‘Arapaho’和‘Boysen’果肉木质素分别在花后24 d和花后27 d含量较高。【结论】‘Arapaho’和‘Boysen’果实着色后,RuCADs基因成员表达呈多样化,且硬度与RuCAD酶及木质素含量关系表现出一定差异; 而在果实发育后期,RuCADs基因表达量降低。CAD酶活性降低及木质素含量下降,与黑莓成熟果实硬度下降具一致性,推测木质素合成与成熟果实硬度形成具有相关性。
Abstract:
【Objective】 This study aimed to investigate the relationships among the expression profiles of cinnamyl alcohol dehydrogenase(CAD)genes, CAD enzyme activity, and total lignin content in fruit flesh from the fruit-setting to the mature stage of blackberry(Rubus spp.)cv. ‘Arapaho’ and ‘Boysen’, which had relatively high and low firmness, respectively. 【Method】 Expression profiles of RuCAD genes in the fruit flesh of the two blackberry cultivars during the stages from coloring to maturity were detected using semi-quantitative RT-PCR. The changes in CAD enzyme activity and the lignin contents in fruit flesh were determined by colorimetric and titration methods, respectively. 【Result】 During fruit development of the two cultivars, eight RuCAD genes exhibited high expression level in the early and middle stages. RuCAD16 was only detected in the early and middle stages in ‘Boysen’ fruit flesh and not found in ‘Arapaho’ during any developmental stage. CAD enzyme activitly level in ‘Arapaho’ and ‘Boysen’ fruits was higher before coloring, specifically 9 DAF(days after flowering)and 21 DAF, and both declined after coloring. Total lignin content in the fruit flesh of both cultivars increased to relatively high level at 24 DAF and 27 DAF, respectively. 【Conclusion】After fruit coloring, expression level of RuCAD genes exhibited various patterns, and the relationships among the fruit firmness, CAD enzyme activity and lignin content showed differences in fruits of ‘Arapaho’ and ‘Boysen’. At late developmental stages, the fruit flesh of ‘Arapaho’ and ‘Boysen’ both showed similar declines in the expression level of RuCAD genes, CAD enzyme activity, and total lignin contents, which was consistent with the decline in blackberry fruit firmness at maturity, implying a relationship between lignin synthesis and firmness in ripe fruits.

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备注/Memo

备注/Memo:
收稿日期:2018-05-02 修回日期:2018-05-29基金项目:国家自然科学基金项目(31600553); 江苏省农业科技自主创新项目[CX(17)1004,CX(18)2018]; 江苏现代农业产业技术体系建设项目(JATS[2018]317)。 第一作者:张春红(chzhang@cnbg.net)。*通信作者:吴文龙(1964wwl@163.com),研究员,ORCID( 0000-0002-7630-6255)。引文格式:张春红,熊振豪,吴文龙,等. 黑莓果实发育成熟期木质素合成CAD酶及其基因表达[J]. 南京林业大学学报(自然科学版),2018,42(6):141-148.
更新日期/Last Update: 2019-01-28