[1]方志刚,张立钦,林新春.不同球孢白僵菌菌株DNA的RAPD分析[J].南京林业大学学报(自然科学版),2001,25(04):065-68.[doi:10.3969/j.jssn.1000-2006.2001.04.017]
 FANG Zhi gang,ZHANG Li qin,LIN Xin chun.RAPD Markers of Isolates of 16 Beauveria bassiana[J].Journal of Nanjing Forestry University(Natural Science Edition),2001,25(04):065-68.[doi:10.3969/j.jssn.1000-2006.2001.04.017]
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不同球孢白僵菌菌株DNA的RAPD分析
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
25
期数:
2001年04期
页码:
065-68
栏目:
研究论文
出版日期:
1900-01-01

文章信息/Info

Title:
RAPD Markers of Isolates of 16 Beauveria bassiana
文章编号:
1000-2006(2001)04-0065-04
作者:
方志刚;张立钦;林新春
浙江林学院;浙江临安311300;浙江林学院;浙江临安311300;浙江林学院;浙江临安311300
Author(s):
FANG Zhi gangZHANG Li qinLIN Xin chun
Zhejiang Forestry College, Linan, 311300,China
关键词:
球孢白僵菌DNARAPD标记
Keywords:
B. bassiana DNARAPD marker
分类号:
*
DOI:
10.3969/j.jssn.1000-2006.2001.04.017
文献标志码:
A
摘要:
<正>采用 RAPD技术分析了 1 6个球孢白僵菌菌株的遗传分化。由 1 6 0个随机引物中筛选出 2 7个引物对各菌株进行 PCR扩增。结果表明从 1 6个球孢白僵菌菌株中共获 3 0 1个 RAPD标记 ,其中多态性标记 2 88个 ,占 95.7% ,表明菌株间具有较丰富的遗传多态性。聚类分析把1 6个球孢白僵菌菌株分为三大类群 (1 ) Blh、Bfj、Bgd和 Bpy;(2 ) By7、F- 2 6 3、RECBb0 1、B12 和 B13;(3 ) B7、B11、Bxs、B6 、By2 、Bpc和 Bzs。菌株 DNA多态性与采集地之间有一定的相关性 ,但与寄主来源、孢子形态和对松墨天牛幼虫的毒力间未表现出明显的相关性
Abstract:
primers were selected from 160 primers for RAPD analysis of 16 strains of B. bassiana .Altogether 301 scorable DNA fragments were recorded. Among them, 288 were polymorphic,and accounted for 95.7%.A dendrogram generated by UPGMA method would divide 16 strains into three distinct groups, (1) B lh , B fj , B gd and B py ; (2) B y7 , F 263, RECBb01, B 12 and B 13 ; and (3)B 7,B 11 , B xs , B 6 , B y2 , B pc and B zs . The cluster results were certainly associated with geographic regions, rather than original hosts, conidiospore size and virulence on Monochamus alternatus larvae.

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更新日期/Last Update: 1900-01-01