[1]伊艳杰,黄莹,尚富德.利用RAPD标记研究桂林桂花品种间的亲缘关系(英文)[J].南京林业大学学报(自然科学版),2004,28(07):065-70.[doi:10.3969/j.jssn.1000-2006.2004.07.010]
 YI Yan-jie,HUANG Ying,SHANG Fu-de.Identifying Osmanthus fragrans Cultivars in Guilin City and Evaluating Their Genetic Relationships by RAPD Markers[J].Journal of Nanjing Forestry University(Natural Science Edition),2004,28(07):065-70.[doi:10.3969/j.jssn.1000-2006.2004.07.010]
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利用RAPD标记研究桂林桂花品种间的亲缘关系(英文)
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
28
期数:
2004年07期
页码:
065-70
栏目:
研究论文
出版日期:
1900-01-01

文章信息/Info

Title:
Identifying Osmanthus fragrans Cultivars in Guilin City and Evaluating Their Genetic Relationships by RAPD Markers
文章编号:
1000-2006(2004)S1-0070-01
作者:
伊艳杰;黄莹;尚富德
河南大学;河南开封475001;桂林黑山植物园;广西桂林541002;河南大学;河南开封475001
Author(s):
YI Yan-jieHUANG YingSHANG Fu-de
Henan University,Kaifeng 475001,China,Gulin Heishan Botanical Garden,Guilin 541002,China
关键词:
RAPD桂林桂花亲缘关系
Keywords:
RAPDGuilinOsmanthus fragrans Lour.Genetic relationship
DOI:
10.3969/j.jssn.1000-2006.2004.07.010
文献标志码:
A
摘要:
<正>采用随机扩增多态性DNA(RAPD)技术,从100个随机引物中筛选出扩增效果较好的20个引物,分析桂林市23个桂花品种的基因组多态性。结果表明,20个随机引物共检测到193个位点,其中多态位点为114个,占59.1%。根据扩增结果对这些品种进行聚类分析,并构建出了树状聚类图,可将这些品种划分为4个品种群,与传统分类学结果一致。此项研究表明,以基因型为基础分析桂花品种间的区别是可行的。因此,RAPD技术为解决桂林市的桂花品种分类问题提供了重要依据。
Abstract:
Random Amplified Polymorphic DNA(RAPD) markers were applied to analyze 23 Osmanthus fragrans cultivars in Guilin City. Among the total 193 fragments generated by 20 selected primers (among 100 primers) , 114 appeared to be polymorphic(59. 1%). Cluster analysis based on the RAPD results was performed and dendrogram was constructed. These cultivars tested by RAPD were divided into 4 cultivar groups. The result was consistent with that from traditional taxonomy analysis. The RAPD study illustrated that it is possible to analyze intra-cultivar variation of O. fragrans cultivars on the basis of genotype rather than phenotype and therefore, offered significant evidences in solving taxonomic problem of O. fragrans cultivars in Guilin City.

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更新日期/Last Update: 1900-01-01