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桂花品种的ISSR-PCR分析
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
28
期数:
2004年07期
页码:
071-75
栏目:
研究论文
出版日期:
1900-01-01

文章信息/Info

Title:
A ISSR-PCR Analyze of the Osmanthus fragrans Cultivars
文章编号:
1000-2006(2004)S1-0076-01
作者:
胡绍庆;邱英雄;吴光洪;宣子灿
杭州植物园;浙江杭州310013;浙江大学;浙江杭州310027;杭州市园林绿化工程有限公司;浙江杭州310030;杭州市园林绿化工程有限公司;浙江杭州310030
Author(s):
Hu Shao-qingQiu Ying-xiongWu Guang-hongXuan zi-can
Hangzhou Botanical Garden,Hangzhou 310013,China,Zhejiang University; Hangzhou 310027,China, Hangzhou Landscape Engineering Co.,Ltd,Hongzhou 310030,China
关键词:
桂花ISSR品种鉴定遗传关系(遗传多样性)
Keywords:
Osmanthus fragransISSRCultivar identificationGenetic relationship (genetic diversity)
DOI:
10.3969/j.jssn.1000-2006.2004.07.011
文献标志码:
A
摘要:
<正>利用ISSR—PCR方法对桂花的54个品种进行了基因组多态性分析,从70个ISSR引物中筛选出13个多态性引物用于正式扩增,共扩增出90条DNA片段,其中多态性DNA带79条,占总扩增片段的87.8%,平均每个引物扩增的DNA带的数目为6.92条。依据扩增结果,应用RAPD Distans软件进行Nei相似性系数和遗传距离计算,利用UPGMA法构建聚类树状图。结果表明:ISSR分析中产生了一些品种特有的指纹图谱,因此ISSR技术在桂花品种和品种群(品系)的鉴定和阐明遗传关系中有很大的应用潜力。利用DNA扩增结果进行聚类分析,把供试桂花的54个品种分为7个大类,并对品种进行了处理及种下品种群的遗传关系进行了探讨。
Abstract:
In this study, inter-simple sequence repeat (ISSR) was evaluated for its potential use in the identification of 54 Osmanthus fragrans cultivars. 13 out of 70 (18. 6%) ISSR primers could generate reproducible polymorphic fragments. The ISSR-PCR assay revealed a total number of 90 DNA bands, of which 79 bands were polymorphic (the percentage of polymorphic bands, PPB=89. 9%). Optimized ISSR primers amplified 4 to 10 bands ranging in size from 300 bp to 2000 bp, with an overall average of 6. 92 amplified bands per primer. Additionally, ISSR produced 1 cultivar-specific molecular marker. AMOVA(Analysis of molecular variance) software was used to calculate the Nei’s genetic distance and a dendrogram was constructed based on UPGMA cluster analysis. These 54 sweet osmanthus cultivars surveyed were classified into 7 major groups, and each cultivar in this study could be distinguished from others, suggesting that PCR-based ISSR analysis was an efficient method for cultivar identification. The efficiency of ISSR analysis for cultivar identification and classification of sweet olive were also discussed.

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更新日期/Last Update: 1900-01-01