[1]刘龙昌,向其柏,刘玉莲.RAPD标记在桂花遗传多样性检测和品种鉴定中的应用(英文)[J].南京林业大学学报(自然科学版),2004,28(07):076-82.[doi:10.3969/j.jssn.1000-2006.2004.07.012]
 LIU Long-chang,XIANG Qi-bai,LIU Yu-lian.The Application of RAPD Markers in Diversity Detection and Cultivars Identification of Osmanthus fragrans[J].Journal of Nanjing Forestry University(Natural Science Edition),2004,28(07):076-82.[doi:10.3969/j.jssn.1000-2006.2004.07.012]
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RAPD标记在桂花遗传多样性检测和品种鉴定中的应用(英文)
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
28
期数:
2004年07期
页码:
076-82
栏目:
研究论文
出版日期:
1900-01-01

文章信息/Info

Title:
The Application of RAPD Markers in Diversity Detection and Cultivars Identification of Osmanthus fragrans
文章编号:
1000-2006(2004)S1-0081-01
作者:
刘龙昌;向其柏;刘玉莲
南京林业大学;江苏南京210037新疆农业大学,新疆乌鲁木齐830052;南京林业大学;江苏南京210037;南京林业大学;江苏南京210037
Author(s):
LIU Long-changXIANG Qi-baiLIU Yu-lian
Nanjing Forestry University,Nanjing 210037,China;Xinjiang Agricultural University,Urumqi 830052,China
关键词:
桂花品种资源RAPD分析
Keywords:
Osmanthus gragransCultivars identificationRAPD
DOI:
10.3969/j.jssn.1000-2006.2004.07.012
文献标志码:
A
摘要:
<正>从100个随机引物中筛选出28个多态性稳定的引物,对34个桂花品种、6个桂花野生居群个体、5个木犀属其他种进行了遗传多样性和亲缘关系的RAPD分析。共检测了231个位点,其中209个位点为多态位点(90.4%)。不同品种群的多态性位点频率差异明显,按大小依次排序为:银桂品种群>四季桂品种群、金桂品种群>丹桂品种群。聚类结果与形态分类结果不完全一致。利用来自引物A11和M18扩增的21条多态性条带构建了桂花品种的DNA指纹图谱,该图谱可以方便地应用于桂花品种的鉴定。不同桂花品种之间存在明显差异。研究结果表明,RAPD技术可用于桂花品种的鉴定和亲缘关系的探讨。
Abstract:
Based on 28 Primers which are of stable polymorphism selected from 100 random primers, RAPD polymorphism of 34 sweet osmanthus(Osmanthus fragrans)cultivars, 6 individuals from wild population and 5 other species of Osmanthus, and their relationships were detected. Among a total of 231 fragments observed, 209 fragments were polymorphic (90.4%). There were obvious differentiation among different goup in polymorphism, and the order was as follows: Latifolius Group>Fragrans Group and Thunbergii Group>Auran-tiacus Group. The results of cluster analysis were consistent with morphological classification by and large. A DNA fingerprinting based on 21 bands amplified with All and M18 was constructed and might be used in sweet osmanthus cultivars identification. There were obvious differentiaion among different cultivars . In addition, it is feasible to identify the sweet osmanthus cultivars and to study their relationships of this cultivars by use of the RAPD technique.

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更新日期/Last Update: 1900-01-01