[1]杨立伟,施季森’.杉木茎段形成层组织cDNA文库的构建[J].南京林业大学学报(自然科学版),2005,29(06):085-87.[doi:10.3969/j.jssn.1000-2006.2005.06.021]
 YANG Li-wei,SHI Ji-sen*.Construction, of cDNA Library from Stem Cambium Tissue of Cuninghamia lanceolata (Lam.) Hook[J].Journal of Nanjing Forestry University(Natural Science Edition),2005,29(06):085-87.[doi:10.3969/j.jssn.1000-2006.2005.06.021]
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杉木茎段形成层组织cDNA文库的构建
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
29
期数:
2005年06期
页码:
085-87
栏目:
研究论文
出版日期:
2005-10-20

文章信息/Info

Title:
Construction, of cDNA Library from Stem Cambium Tissue of Cuninghamia lanceolata (Lam.) Hook
文章编号:
1000-2006(2005)06-0085-03
作者:
杨立伟施季森’
国家林业局林木遗传与基因工程重点实验室南京林业大学, 江苏 南京 210037
Author(s):
YANG Li-wei SHI Ji-sen*
The Key Laboratory of Forest Genetics and Engineering State Forestry Adrnnistration Nanjing Forestry University, Nanjing 210037, China
关键词:
杉木 茎段 形成层组织 cDNA文库
Keywords:
Cuninghamia lanceolata (Lain.) Hook Stem Cambium tissue cDNA library
分类号:
S722; Q813
DOI:
10.3969/j.jssn.1000-2006.2005.06.021
文献标志码:
A
摘要:
<正>通过不同地点、不同时期采集桉树枯落物样品,对其进行微生物的分离、纯化与鉴定。分离共获得195株微生物菌株,其中细菌129株分属于10属,主要为芽孢杆菌属(Bacillus)、假单胞杆菌属(Pseudomonas)、利斯特菌属(Listeria)等;放线菌23株分属于链霉菌属(streptomyces)、诺卡氏菌属(Nocardia)、小单孢菌属(Micromonospora);真菌44株分属于12个属,主要为镰刀菌属(Fusarium)、头孢霉属(Cephalos-porium)、丝核菌属(Rhizoctonia)等。分析表明:桉树枯落物中微生物数量和种类丰富,表现为细菌数量最高,真菌次之,放线菌最少;不同时期三大菌数量变化不明显;另外,混交林中的三大菌数量比纯桉林中的高。
Abstract:
In order to isolate target genes for controlling the wood formation and quality components, a cDNA library was constructed from Cuninghamia lanceolata stems cambium tissue with the ZAP express phage vector. The host bacteria is E. coli XL1-Blue, and subclone bacteria is E. coli XLOLR. The compacity of primary library is 6.1 X l0s pfu, and the titer of amplified library is 5.2×109 pfu/mL, in which 95% of phages were recombinant, the size of fragments inserted rang from 0.5 kb to 3.0 kb.

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更新日期/Last Update: 2013-05-20