[1]席梦利,施季森*.杉木合子胚愈伤组织的诱导及植株再生[J].南京林业大学学报(自然科学版),2006,30(02):006-10.[doi:10.3969/j.jssn.1000-2006.2006.02.002]
 XI Meng-li,SHI Jisen*.Callus Induction and Plantlets Regeneration from Zygotic Embryos of Cunninghamia lanceolata Hook.[J].Journal of Nanjing Forestry University(Natural Science Edition),2006,30(02):006-10.[doi:10.3969/j.jssn.1000-2006.2006.02.002]
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杉木合子胚愈伤组织的诱导及植株再生
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
30
期数:
2006年02期
页码:
006-10
栏目:
研究论文
出版日期:
2006-02-20

文章信息/Info

Title:
Callus Induction and Plantlets Regeneration from Zygotic Embryos of Cunninghamia lanceolata Hook.
文章编号:
1000-2006(2006)02-0006-05
作者:
席梦利施季森*
国家林业局、江苏省林术遗传和基因工程重点实验室南京林业大学, 江苏 南京 210037
Author(s):
XI Meng-li SHI Jisen*
The Key Laboratory of Forest Genetics and Gene Engineering of the State Forestry Administration and Jiangsu Province Nanjing Forestry University, Nanjing 210037, China
关键词:
杉木 愈伤组织 植株再生
Keywords:
Cunninghamia lanceolata Hook. Callus Plantlets regeneration
分类号:
S722.3+7
DOI:
10.3969/j.jssn.1000-2006.2006.02.002
文献标志码:
A
摘要:
<正>从复合材料力学理论出发,建立了不对称人造板耦合刚度矩阵计算模型,讨论了耦合刚度系数B11、B22与板坯翘曲度的关系,分析了板坯层数、铺层顺序和铺设方向等对板坯耦合效应的影响。结果表明:板坯耦合刚度矩阵B中各元素绝对值越大,其翘曲度越大;在B矩阵中,系数B11和B22值对板坯翘曲度影响较大。可以通过改变板的层数、铺层顺序及铺设方向等因素来进行不对称人造板的结构设计,减少板坯的翘曲度。
Abstract:
Using mature zygotic embryos and immature zygotic embryos as initial explants, the effect of the following factors on induction callus and differentiation adventitious buds.. basal medium, plant hormone and development stages of zygotic embryo were studied. Results indicated that the optimal medium for mature zygotic embryos induction callus was 1/2MS+2mg/L 2,4-D+1.0mg/L 6-BA+1. 0mg/L KT+30 g/L sucrose. This medium can induce soft, transparent callus from immature zygotic embryos of embryo selection or tissue and organ differentiation stages. The induction frequency was 2%~6%. Immature zygotic embryos of tissue and organ differentiation or embryo mature stages induction callus were similar in state with those of mature zygotic embryo. The callus induction frequency was significantly improved with the maturity of immature zygotic embryos. The optimal medium for differentiation adventitious buds was DCR+1.5mg/L 6-BA+1. 5mg/L KT+20 g/L sucrose. Shoots with 1. 5 cm high were transfered to rooting medium and rooting frequency reached 100%.

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更新日期/Last Update: 2013-05-20