[1]吕月良,陈璋,施季森*,等.福建山樱花不定芽诱导和植株再生规模化繁殖试验[J].南京林业大学学报(自然科学版),2006,30(03):105-108.[doi:10.3969/j.jssn.1000-2006.2006.03.024]
 Lv Yue-liang,CHEN Zhang,SHI Ji sen*,et al.Adventitious Bud Inducing and Plant Regeneration of Cerasus campanulata Maxim. in Large Scale[J].Journal of Nanjing Forestry University(Natural Science Edition),2006,30(03):105-108.[doi:10.3969/j.jssn.1000-2006.2006.03.024]
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福建山樱花不定芽诱导和植株再生规模化繁殖试验
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
30
期数:
2006年03期
页码:
105-108
栏目:
研究论文
出版日期:
2006-03-20

文章信息/Info

Title:
Adventitious Bud Inducing and Plant Regeneration of Cerasus campanulata Maxim. in Large Scale
文章编号:
1000-2006(2006)03-0105-04
作者:
吕月良12陈璋2施季森1*黄宇翔3刘金燕3谢建丽3
1. 国家林业局、江苏省林木遗传基因工程重点实验室南京林业大学, 江苏 南京 210037; 2. 福建省林业厅, 福建 福州 350003; 3. 福建省农业科学院作物研究所, 福建 福州 350013
Author(s):
Lv Yue-liang12 CHEN Zhang2 SHI Ji sen1* HUANG Yu-xiang3 LIU Jin-yan3 XIE Jian-li3
1. The Key Laboratory of Forest Genetics and Gene Engineering of the State Forestry Administration and Jiangsu ProvinceNaniing Forestry University, Nanjing 210037, China; 2. Forestry Administration of Fujian Province, Fuzhou 350003, China; 3. Crop Institute of Fujian Academy of Agriculture, Fuzhou 350013, China
关键词:
福建山樱花 组织培养 不定芽 植株再生 规模化生产
Keywords:
Cerasus campanulata Tissue culture Adventitious bud Plant regeneration Masspropagation
分类号:
S722
DOI:
10.3969/j.jssn.1000-2006.2006.03.024
文献标志码:
A
摘要:
<正>研究了切根和截顶对湿地松苗木质量的影响,结果表明:(1)不同的切根处理对湿地松苗高和地径的生长有显著抑制作用,几种处理中,以9月份切根对苗高的抑制最小,以3次切根的处理对地径生长的抑制作用最小,但切根处理对湿地松苗木的高径比影响较小;(2)几种切根处理中,只有1次切根处理下地上部分的干重大干对照,2次切根处理根系干重最小。全苗千重以1次处理时最大,其次为对照;(3)不同的截顶处理下,湿地松苗高和地径生长都比对照低,单次截顶的苗高均比2坎截顶的高,地径生长以9月中旬处理的较大;(4)几种截顶处理中。根干重和侧须根干重都比对照低,其中8月中旬截顶处理下苗木生长最慢,单次截顶处理的侧主根干重均大于对照,全苗干重都明显小于对照。
Abstract:
The explants in 4 em length collected from spring shoots of 3-year-old selected tree of Cerasus carnpanulata were inoculated on MS culture medium with 6-BA and IBA for adventitious buds mutiplication. The results showed that explants were prevented effectively from being brown and dying when the medium of 1/4MS were used. Explants grew fast and no vitrification when the medium plused with 1.0 mg/L 6-BA and 0.01 mg/L IBA. The medium of MS with 1.0 mg/L 6-BA and 0.3 mg/L GA3 was the best for multiplication and multiplication rate could reach to 600% during one culture cycle. Plantlets were to be transferred on the 1/2MS medium with 1.0 mg/L NAA and 0.2 mg/L IBA for rooting, when the explants were reached in about 4 cm. The root system could be developed well during 25 days and to be come a new plant. The rooting rate could reach to 92.1% after 35 days. The survival rate was above 94% when the plantlets were to be moved to nursery. The medium in 340//00 pearlite and 67%peat soil was good for rooting. This micropropagation technology could be applied in mass propagation.

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更新日期/Last Update: 2013-05-20