[1]朱丽梅,吴小芹*,蒋 萍.细菌XSJS3对松材线虫杀线活性的测定[J].南京林业大学学报(自然科学版),2008,32(01):083.[doi:10.3969/j.jssn.1000-2006.2008.01.019]
　ZHU Li-mei,WU Xiao-qin*,JIANG Ping.A study of the nematicidal activity of bacterium XSJS3 to Bursaphelenchus xylophilus[J].Journal of Nanjing Forestry University(Natural Science Edition),2008,32(01):083.[doi:10.3969/j.jssn.1000-2006.2008.01.019]
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细菌XSJS3对松材线虫杀线活性的测定
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
32
期数:
2008年01期
页码:
083
栏目:
研究论文
出版日期:
2008-01-30

文章信息/Info

Title:
A study of the nematicidal activity of bacterium XSJS3 to Bursaphelenchus xylophilus
文章编号:
1000-2006 (2008) 01-0079-04
作者:
朱丽梅1; 2; 吴小芹1*; 蒋 萍1; 3
1.南京林业大学森林资源与环境学院,江苏 南京 210037
2.金陵科技学院园艺学院,江苏 南京 210038
3.新疆农业大学林学院,新疆 乌鲁木齐 830052
Author(s):
ZHU Li-mei1; 2;  WU Xiao-qin1*;  JIANG Ping1; 3
1.College of Forest Resources and Environment Nanjing Forestry University,Nanjing 210037, China
2. College of Gardening,Jinling Institute of Technology,Nanjing 210038, China
3.College of Forestry Xinjiang Agricultural University, Urmqi 830052, China
关键词:
细菌; 松材线虫; 死亡率; 生物防治
分类号:
S763
DOI:
10.3969/j.jssn.1000-2006.2008.01.019
文献标志码:
A
摘要:
细菌XSJS3是一种新发现的对松材线虫(Bursaphelenchus xylophilus)具有较高杀线活性的细菌,试验比较了细菌XSJS3培养液、细菌培养液滤液及含菌体水溶液对松材线虫的杀线活性,结果发现细菌菌体本身对松材线虫无杀线活性,杀线活性物质存在于细菌的培养液滤液中。通过对该细菌培养液滤液对松材线虫致死过程的观察发现:松材线虫接触该细菌培养液滤液30min后就表现为行动迟缓;在5h后所有虫体均不活动,针刺无反应;24h后部分僵直虫体出现局部膨大,且膨大部位出现体液外渗而虫体最终被消解。对不同培养时间的细菌XSJS3滤液杀线活性的比较试验表明:该细菌分泌的杀线物质在10d内随培养时间的延长而含量增加,杀线活性逐渐提高,不同培养时间下滤液的杀线活性差异明显；但培养15d后,稀释10倍的滤液对松材线虫杀线活性比培养10d时同样滤液的杀线活性则略有下降,但差异不明显,因此获得该细菌分泌的杀线物质较理想的培养时间为10d。将细菌XSJS3培养液滤液进行不同温度的热处理后测定对松材线虫的杀线活性,结果表明引起松材线虫死亡和消解的物质是两个不同物质,存在于培养液滤液中的对松材线虫有杀线活性的物质是热稳定的,而引起松材线虫虫体消解的物质是热不稳定的。

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备注/Memo

备注/Memo:
收稿日期: 2006-11-20
基金项目：国家林业局“948”创新项目(2003-4-38)
作者简介: 朱丽梅(1972-),女,副教授,博士生。*通讯作者:吴小芹,女,教授,博士生导师,主要研究方向森林保护学，xqwy@njfu.edu.cn.
更新日期/Last Update: 2008-03-19