[1]宿静,汤庚国*,王光萍,等.美国月桂樱的组织培养及植株再生[J].南京林业大学学报(自然科学版),2008,32(04):098-100.[doi:10.3969/j.jssn.1000-2006.2008.04.022]
 SU Jing,TANG Geng-guo *,WANG Guang-ping,et al.Tissue culture and plant regeneration of Laurocerasus caroliniana Ait.[J].Journal of Nanjing Forestry University(Natural Science Edition),2008,32(04):098-100.[doi:10.3969/j.jssn.1000-2006.2008.04.022]
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美国月桂樱的组织培养及植株再生
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
32
期数:
2008年04期
页码:
098-100
栏目:
研究论文
出版日期:
2008-07-20

文章信息/Info

Title:
Tissue culture and plant regeneration of Laurocerasus caroliniana Ait.
文章编号:
1000-2006(2008)04-0098-03
作者:
宿静汤庚国*王光萍刘雅帅
南京林业大学森林资源与环境学院, 江苏 南京 210037
Author(s):
SU Jing TANG Geng-guo * WANG Guang-ping LIU Ya-shuai
College of Forest Resources and Environment Nanjing Forestry University, Nanjing 210037, China
关键词:
美国月桂樱 组织培养 植株再生
Keywords:
Laurocerasus carotiniana Air. Tissue culture Plant regeneration
分类号:
S722.3
DOI:
10.3969/j.jssn.1000-2006.2008.04.022
文献标志码:
A
摘要:
<正>以油脂下脚料酸化油为原料,通过与顺丁烯二酸酐加成,再与二甘醇酯化,得到聚酯多元醇,其最佳工艺条件为:M(酸化油):M(顺丁烯二酸酐):M(二甘醇)=1:1:3.3;顺丁烯二酸酐在180℃、195~200%:、200~220℃:分3次加入,备反应1h,在230~240℃下反应0.5h;240%:下常压酯化10h;240℃减压酯化1h。合成的聚酯多元醇酶值小于1mg/g。羟值在250~300mg/g。以合成的聚酯多元醇为原料,制备的双组分聚氨酯胶粘剂性能可以达到彩钢板与聚苯乙烯泡沫塑料的胶合工艺要求。
Abstract:
The fasciculated shoots were induced by the explant achieved from the juvenile branches of Laurocerasus caroliniana Air. in spring. The inducing rate of fasciculated shoots attained 100%when they were cultured on the inducing medium of MS with BA O. 5 mg/L and NAA 0. O1 mg/L. The adventitious shoots were propagated most rapidly when subcuhured on the medium of MS with BA 1.0 mg/L and NAA 0.1 mg/L. Moreover,the adventitious shoots with best quality were obtained when they were cultured in the medium of MS with KT I. 0 mg/L and NAA 0. 1 mg/L. The results of this experiment also indicated that gibberellin had no effects on the elongation of adventitious shoots. When the 3--4 cm adventitious shoots were cut and rooted in the rooting medium of 1/2 MS with NAA 1. 0 mg/L,the rooting rate and surviving rate attained 100%and 40%respectively.

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更新日期/Last Update: 2013-05-20