[1]沈艳华,徐锡增,方升佐*,等.硅对盐胁迫下杨树叶片细胞超微结构的影响[J].南京林业大学学报(自然科学版),2009,33(01):049-53.[doi:10.3969/j.jssn.1000-2006.2009.01.010]
 SHEN Yan hua,XU Xi zeng,FANG Sheng zuo,et al.Effects of silicon on cell ultrastructure of poplar leaves under salt stress[J].Journal of Nanjing Forestry University(Natural Science Edition),2009,33(01):049-53.[doi:10.3969/j.jssn.1000-2006.2009.01.010]
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硅对盐胁迫下杨树叶片细胞超微结构的影响
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
33
期数:
2009年01期
页码:
049-53
栏目:
研究论文
出版日期:
2009-01-30

文章信息/Info

Title:
Effects of silicon on cell ultrastructure of poplar leaves under salt stress
作者:
沈艳华徐锡增方升佐* 杨万霞 尚旭岚
南京林业大学森林资源与环境学院,江苏南京210037
Author(s):
SHEN Yanhua XU Xizeng FANG Shengzuo YANG Wanxia SHANG Xulan
College of Forest Resource and Environment, Nanjing Forestry University, Nanjing 210037, China
关键词:
杨树盐胁迫叶绿体超微结构
Keywords:
poplarsiliconsalt stresschloroplastultrastructure
分类号:
S718
DOI:
10.3969/j.jssn.1000-2006.2009.01.010
文献标志码:
A
摘要:
研究了杨树在不同程度盐胁迫下加硅处理30d后,叶片细胞超微结构的变化。结果表明,无硅条件下,随着盐胁迫浓度的增加和时间的延长,杨树叶肉细胞内叶绿体类囊体扭曲、变形、松散,嗜锇颗粒增多,外膜逐渐断裂解体,局部消失,细胞核固缩,核物质凝聚,核膜不清晰。盐质量浓度为40g/L比盐质量浓度为20g/L和30g/L的处理对叶片细胞结构的损伤程度更为严重。盐胁迫下加硅后杨树叶肉细胞细胞器损伤得到明显改善,表现为叶绿体类囊体排列有序,嗜锇颗粒略有增多,细胞核基本完好,局部核物质凝聚。施硅质量浓度为05g/L比施10g/L的效果显著,且对盐质量浓度为20g/L和30g/L的缓解效果比质量浓度为40g/L的更为明显。
Abstract:
The effects of silicon on cell ultrastructure of poplar leaves were investigated under salt stress for 30 days. The results showed that in the salt treatments without adding silicon, the slices of basal granule distorted or even disintegrated, the quantity of osmiophilic granule increased, the membrane of chloroplast became disintegrated and some of them disappeared with increasing external NaCl concentration and prolonging treatment time. Under salt stress,the cell nucleus were also condensed, and the nuclear membrane could not be seen clearly. The phenomena mentioned above was more obvious in the treatment with 4.0g/L NaCl than that in the 2.0g/L and 3.0g/L salt treatment. However, in the salt treatments with the addition of silicon, the degeneration of membrane systems decreased, and the lamellae were lined regularly, while the chloroplast grana lamellae just began to degenerate and the number of osmiophilic granule were increased more or less,even if some cell nucleus were condensed. The study also indicated that effects on mitigating salt stress were more significant in the treatment with silicon addition of 0.5g/L than in the addition of 1.0g/L. Moreover, the mitigating effects were more obvious in the 2.0,3.0g/L salt treatments than that in the 4.0g/L treatment.

参考文献/References:

[1]刘友良,章文华,丁念诚,等.大麦耐盐机理的研究[C]//中国大麦文集:第三集.南昌:江西科学技术出版社,1993.
[2]孔令安,郭洪海,董晓霞.盐胁迫下杂交酸模超微结构的研究[J].草业学报, 2000, 9(2):5357.
[3]Katsuhara M, Kawasaki T. Salt stress induced nuclear and DNA degradation in meristematic cell of barley roots[J]. Plant Cell Physiol,1996,37:169173.
[4]束良佐,刘英慧.硅对盐胁迫下玉米幼苗叶片膜脂过氧化和保护系统的影响[J].厦门大学学报,2001,40(6):12951300.
[5]陈阳,王贺,张福锁,等.硅盐互作下小獐毛植物体内元素分布及生理特性的研究[J].植物生态学报,2003,27(2):195198.
[6]梁永超,丁瑞兴.硅对大麦根系中离子的微域分布的影响及其与大麦耐盐性的关系[J].中国科学:C辑,2002,32(2):113121.
[7]Liang Y C. Effect s of silicon on leaf ultrastructure, chlorophyll content and photosynthetic activity in barley under salt stress[J].Pedosphere,1998,8:289296.
[8]徐呈祥,刘友良,马艳萍.硅对盐胁迫下库拉索芦荟叶绿素荧光参数和叶绿体超微结构的影响[J].园艺学报,2007,34(4):979984.
[9]郑文菊,张承烈.盐生和中生环境中宁枸杞叶显微和超微结构的研究[J].草业科学,1998,7(3):7276.
[10]闫先喜,赵檀方,胡延吉. 盐胁迫预处理对大麦根尖分生区细胞超微结构的影响[J]. 西北植物学报,1994,14(4):2732771.
[11]王羽梅,任安详,潘春香. 长时间盐胁迫对苋菜叶片细胞结构的影响[J].植物生理学通讯,2004,40(3):2892921.
[12]刘永霞,徐锡增.硅对盐胁迫下‘金丝小枣’叶片超微结构的影响[J].林业科技开发,2007,21(4):3942.

[13]秘彩莉,黄占景,邵素霞,等.近似等位基因系小麦盐胁迫下叶绿体超微结构下比较研究[J].电子显微学报,2001,20(2):98101.
[14]王波,宋凤斌,任长忠,等.盐碱胁迫对燕麦叶绿体及一些生理指标的影响[J].吉林农业大学学报,2005,27(5):473477,485.

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备注/Memo

备注/Memo:
收稿日期:2008-01-11修回日期:2008-05-21 基金项目:高等学校博士学科点专项科研基金资助项目(20050298011); 国家“十一五”科技支撑计划(2006BAD24B0402) 作者简介:沈艳华(1982—),硕士生。*方升佐(通讯作者),教授,研究方向为人工林定向培育和能源林培育。Email:Fangsz@njfu.com.cn 引文格式:沈艳华,徐锡增,方升佐,等.硅对盐胁迫下杨树叶片细胞超微结构的影响[J].南京林业大学学报:自然科学版,2009,33(1):4953.
更新日期/Last Update: 2009-05-06