[1]高景斌,徐六一,叶建仁*,等.运用MuPS标记识别马尾松抗松材线虫病个体[J].南京林业大学学报(自然科学版),2009,33(03):001-4.[doi:10.3969/j.jssn.1000-2006.2009.03.001]
 GAO Jing bin,XU Liu yi,YE Jian ren*,et al.Using MuPS marker technique to identify individual of Pinus massoniana resistance to pine wilt disease[J].Journal of Nanjing Forestry University(Natural Science Edition),2009,33(03):001-4.[doi:10.3969/j.jssn.1000-2006.2009.03.001]
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运用MuPS标记识别马尾松抗松材线虫病个体
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
33
期数:
2009年03期
页码:
001-4
栏目:
研究论文
出版日期:
2009-05-30

文章信息/Info

Title:
Using MuPS marker technique to identify individual of Pinus massoniana resistance to pine wilt disease
作者:
高景斌12徐六一2叶建仁1*矶田圭哉3生方正俊3
1.南京林业大学森林资源与环境学院,江苏南京210037;2.安徽省松材线虫抗性育种中心,安徽合肥230031; 3.日本森林综合研究所林木育种中心,日本茨城县3191301
Author(s):
GAO Jingbin12 XU Liuyi2 YE Jianren1* ISODA Keiya3 UBUKATA Masatoshi3
1.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China; 2.Anhui Province Forestry Bureau Resistance Breeding for Pine nematode Centre, Hefei 230031, China; 3.Forest Tree Breeding Center Forestry Research Products Research Institute, Ibaraki 3191301, Japan
关键词:
松材线虫马尾松抗性选育MuPS
Keywords:
Bursaphelenchus xylophilus Pinus massoniana resistance breeding MultiplexPCR of SCAR markers
分类号:
S763
DOI:
10.3969/j.jssn.1000-2006.2009.03.001
文献标志码:
A
摘要:
运用14个SCAR标记引物对松材线虫病马尾松(Pinus massoniana)抗性候选个体开展MuPS(MultiplexPCR of SCAR markers)分析。结果表明,120个样品中出现104个不同的MuPS型,区分率86.7%、74.2%的个体完全能够被识别。引物Pmscar157、Pmscar007、Pmscar053的多态位点数出现率非常高,分别占总位点数的131%、13.3%、18.4%,而引物Pmscar039、Pmscar499的位点数出现率非常低,分别占总位点数的0.16%、048%。因引物重复性高、稳定可靠,可通过获得的MuPS型识别抗性候选个体、管理抗性候选家系。
Abstract:
Using 14 SCAR marker primers to analyze the resistance seedlings of Pinus massoniana which resist Bursaphelenchus xylophilus. The results indicated that there were 104 different types of MuPS among those 120 samples, which could distinguish 86.6% samples, and 74.2% samples identified completely. Some primers such as Pmscar157, Pmscar007 and Pmscar053 produced more bands, which was 13.1%, 13.3%, 18.4% of the total bands respectively, while the primers Pmscar039 and Pmscar499 produced less band, and they were 0.16% and 0.48% of the total bands respectively. The primers used in this paper could fingerprint well because of high stability and reliability. Therefore, the attained MuPS could be used to identify and manage the resistance individual and the resistance family of P.massoniana.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2008-09-03修回日期:2008-12-29基金项目:日本国际协力机构(JICA)援助项目作者简介:高景斌(1965—),博士生。*叶建仁(通讯作者),教授,研究方向为森林保护学。Email: jryenanjing@yahoo.com.cn。引文格式:高景斌,徐六一,叶建仁,等. 运用MuPS标记识别马尾松抗松材线虫病个体[J]. 南京林业大学学报:自然科学版,2009,33(3):1-4.
更新日期/Last Update: 2009-05-30