[1]王滑,阎亚波,张俊佩,等.应用ITS序列及SSR标记分析核桃与铁核桃亲缘关系[J].南京林业大学学报(自然科学版),2009,33(06):035.[doi:10.3969/j.jssn.1000-2006.2009.06.008]
 WANG Hua,YAN Ya bo,ZHANG Jun pei,et al.Application of ITS sequence and SSR markers to study the relationship between Juglans regia and Juglans sigillata[J].Journal of Nanjing Forestry University(Natural Science Edition),2009,33(06):035.[doi:10.3969/j.jssn.1000-2006.2009.06.008]
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应用ITS序列及SSR标记分析核桃与铁核桃亲缘关系
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
33
期数:
2009年06期
页码:
035
栏目:
研究论文
出版日期:
2009-11-30

文章信息/Info

Title:
Application of ITS sequence and SSR markers to study the relationship between Juglans regia and Juglans sigillata
作者:
王滑1阎亚波2张俊佩1裴东1*
1.中国林业科学研究院林业研究所,国家林业局森林培育重点实验室,北京100091;2.河南省洛阳市林业局,河南洛阳471000
Author(s):
WANG Hua1 YAN Yabo2 ZHANG Junpei1 PEI Dong1*
1.Institute of Forestry, Key Laboratory Silviculture of the State Forestry Administration, Chinese Academy of Forestry, Beijing 100091, China; 2.Forestry Department of Luoyang Henan Province, Luoyang 471000, China
关键词:
核桃铁核桃ITSSSR亲缘关系
Keywords:
Juglans regia J.sigillata ITS SSR
分类号:
S722
DOI:
10.3969/j.jssn.1000-2006.2009.06.008
文献标志码:
A
摘要:
核桃与铁核桃是我国核桃属两个主要栽培种,而后者又是我国特有种,关于两个种在植物分类学上的归属问题一直存在争议。该研究分别以核桃与铁核桃的5个居群为试材,采用ITS序列多态性分析及SSR分子标记技术,从分子水平上讨论了两个种的亲缘关系。结果显示两个种的ITS序列仅出现了一个碱基差异,序列高度相似。SSR标记共扩出了65个等位基因,其中三家村居群与其宗居群的遗传距离最近,为0.119,遗传距离最远的为MN居群与HS居群,为0.522。种间居群遗传距离与种内遗传距离相近,种间差异不明显。这一结果说明两个种应为不同的地理生态型。
Abstract:
Juglans regia L. and J.sigillata Dode. are two mainlycultivated walnut species in China, and the later is a unique species distributed in the southwest China. Furthermore, the argument about relationship of the two species remains undetermined. Five populations of J.regia and J.sigillata were chosen to investigate their relationship with ITS and SSR markers. Result of ITS sequence only detected 1 nucleotide difference between the two species. Based on 65 alleles generated with SSR, genetic distance between populations was calculated. The closest genetic distance was 0.119 found between SJC and QZ population, and the furthest distance was 0.552 found between MN and HS population. The genetic distance between intraspecies was similar to that between interspecies, and the difference between two species was quite small and was not significant. Those results suggested that the two species might be different ecotypes, which provided a scientific reference for revealing the evolutional and genetic relationship and genetic characteristics of walnut plants.

参考文献/References:

[1]郗荣庭,张毅萍. 中国果树志·核桃卷[M]. 北京:中国林业出版社,1996.
[2]王红霞,张志华,玄立春. 我国核桃种质资源及育种研究进展[J]. 河北林果研究,2007,22(4):387-392.
[3]曲泽洲,孙云蔚. 果树种类论[M]. 北京:农业出版社,1990.
[4]杨自湘,奚声珂. 胡桃属十种植物的过氧化物同工酶分析[J]. 植物分类学报,1989,27 (1):53-57.
[5]匡可任,路安民. 中国植物志:第21 卷[M]. 北京:科学出版社,1979.
[6]吴燕民,裴东,奚声珂,等. 运用RAPD对核桃属种间亲缘关系的研究[J]. 园艺学报,2000,27(1):17-22.
[7]郗荣庭,张毅萍. 中国核桃[M].北京:中国林业出版社,1992.
[8]邹喻萍,葛颂,王晓东. 系统与进化植物学中的分子标记[M]. 北京:科学出版社,2001.
[9]田欣,李德铢. DNA序列在植物系统学研究中的应用[J]. 云南植物研究,2002,24(2):170-184.
[10]Stanford M A, Rachel H, Clifford R P. Phylogeny and biogeography of Juglans (Juglandaceae) based on mATK and ITS sequence data[J]. American Journal of Botany, 2000, 87(6): 872-882.
[11]Mallikarjuna K, Aradhya D P, Gao F Y, et al. Molecular phylogeny of Juglans (Juglandaceae): a biogeographic perspective[J]. Tree Genetics & Genomes, 2007, 3: 363-378.
[12]Lefort F, Douglas G C. An efficient micro–method of DNA isolation from mature leaves of four hardwood tree species Acer, Fraxinus, Prunus and Quercus[J]. Annals of Forest Science, 1999, 56: 259-263.
[13]Woeste K, Burns R, Rhodes O E, et al. Thirty polymorphic nuclear microsatellite loci from black walnut[J]. Journal of Heredity, 2002, 93: 58-60.
[14]Foroni I, Rao R, Woeste K, et al. Characterisation of Juglans regia L. with SSR markers and evaluation of genetic relationships among cultivars and the ’Sorrento’ landrace[J]. J Hort Sci & Biotechnol, 2005, 80(1): 49-53.
[15]Victory E R, Glaubitz J C, Rhodes Jr O E, et al. Genetic homogeneity in Juglans nigra (Juglandaceae) at nuclear microsatellites[J]. American Journal of Botany, 2006, 93(1): 118-126.
[16]Wang Y H, Luo J X, Xue X M. Diversity of microsatellite markers in the populations of Picea asperata originating from the mountains of China[J]. Plant Science, 2005, 168: 707-714.
[17]Panaud O, Chen X, McCouch S D. Development of microsatellite markers and characterization of sample sequence lengthen polymorphism (SSLP) in rice (Oryzastiva L.)[J]. Mol Gen Genet, 1996, 252: 597-607.
[18]Yeh F C, Yang R C, Boyle T. POPGENE version 1.32 ed. Software Microsoft Window–Based Freeware for Population Genetic Analysis[M]. Edmonton, Alta: University of Alberta Press, 1997.
[19]Nei M. Estimation of average heterozygosity and genetic distance from a small number of individuals[J]. Genetics, 1978, 89: 583-590.
[20]WANG H, PEI D, GU R S,et al. Genetic diversity and structure of walnut populations in central and southwestern China revealed by microsatellite markers[J]. Journal of America Society Horticulture Science, 2008, 133(2): 197-203.
[21]路安民,张志耘. 胡桃目的分化、进化和系统关系[J]. 植物分类学报,1990,28(2):96-102.
[22]Peng Y H, Lu Z, Chen K. Population genetic survey of Populus cathayana originating from southeastern Qinghai–Tibetan plateau of China based on SSR markers[J]. Silvae Genetica, 2005, 54(3): 116-122.
[23]范志远,方文亮,杨振邦,等. 南北核桃种间杂交F1代主要性状遗传分析[J]. 云南林业科技,2001(4):31-37.

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备注/Memo

备注/Memo:
收稿日期:2009-02-25修回日期:2009-06-15基金项目:国家“十一五” 科技支撑计划(2006BAD01A1703)作者简介:王滑(1981—),博士生。*裴东(通讯作者),研究员,研究方向为植物生物技术。Email: peigu@caf.ac.cn。引文格式:王滑,阎亚波,张俊佩,等. 应用ITS序列及SSR标记分析核桃与铁核桃亲缘关系[J]. 南京林业大学学报:自然科学版,2009,33(6):35-38.
更新日期/Last Update: 2009-11-30