[1]朱丽梅,吴小芹*,徐旭凌.具杀松材线虫活性的解淀粉芽孢杆菌 JKJS3的培养条件[J].南京林业大学学报(自然科学版),2010,34(05):055-58.[doi:10.3969/j.jssn.1000-2006.2010.05.012]
 ZHU Li mei,WU Xiao qin*,XU Xu ling.The culture conditions of Bacillus amyloliquefaciens strain JKJS3 with the nematicidal activity on Bursaphelenchus xylophilus[J].Journal of Nanjing Forestry University(Natural Science Edition),2010,34(05):055-58.[doi:10.3969/j.jssn.1000-2006.2010.05.012]
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具杀松材线虫活性的解淀粉芽孢杆菌 JKJS3的培养条件
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
34
期数:
2010年05期
页码:
055-58
栏目:
研究论文
出版日期:
2010-10-08

文章信息/Info

Title:
The culture conditions of Bacillus amyloliquefaciens strain JKJS3 with the nematicidal activity on Bursaphelenchus xylophilus
作者:
朱丽梅12吴小芹1*徐旭凌1
1.南京林业大学森林资源与环境学院,江苏省有害生物预防与控制重点实验室,江苏南京210037; 2.金陵科技学院园艺学院,江苏南京210036
Author(s):
ZHU Limei12 WU Xiaoqin1* XU Xuling1
1.College of Forest Resources and Environment, Nanjing Forestry University, Jiangsu Key Laboratory for Pest Prevention and Management of Invasive Specie, Nanjing 210037,China;2.Gardening College of Jinling Institute of Technology, Nanjing 210036,China
关键词:
杀线活性解淀粉芽孢杆菌培养条件松材线虫
Keywords:
nematicidal activity Bacillus amyloliquefaciens culture condition Bursaphelenchus xylophilus
分类号:
S763
DOI:
10.3969/j.jssn.1000-2006.2010.05.012
文献标志码:
A
摘要:
解淀粉芽孢杆菌(Bacillus amyloliquefaciens)JKJS3菌株分离自马尾松林,对松材线虫(Bursaphelenchus xylophilus)具有较高的杀线活性。为进一步开发和应用该菌株,以松材线虫为测试靶标,对JKJS3菌株分泌杀线活性物质的适宜培养基和培养条件进行了研究。结果表明:不同碳源组分的培养基对JKJS3菌株分泌杀线活性物质无显著影响,而不同氮源对其有显著影响,其中以氮源为5 g/L牛肉膏和6 g/L蛋白胨组成的培养基所获培养滤液的杀线活性最高,为实验室条件下菌株分泌杀线活性物质的最适培养基;JKJS3菌株的最佳培养条件为初始pH=7、培养温度为37 ℃和无光照振荡培养;该菌株分泌杀线活性物质较理想的培养时间为10 d。
Abstract:
Bacillus amyloliquefaciens strain JKJS3 was isolated from the needles of Pinus massoniana, which demonstrated with high nematicidal activity on pine wood nematode Bursaphelenchus xylophilus. The culture conditions of strain JKJS3 were studied in the lab. The results showed there were no significant differences in nematicidal effects on B.xylophilus by metabolic materials secreted from JKJS3 under media with different carbon sources, but the significant differences were observed in those with different nitrogen sources.The best nitrogen sources of the culture media is the mixture of beef extract 5 g/L and peptone 6 g/L. The optimal culture condition for secreting nematicidal materials by JKJS3 is shaking cultured the media(pH=7) at 37 ℃ without light. The obvious correlation between the culture time and the nematicidal activity is observed and the optimal culture time is 10 days. The study may provide information for controlling the pine wood nematode by the strain JKJS3 and its nematicidal materials in future.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2009-12-02修回日期:2010-05-17基金项目:国家林业局“948”项目(2003-4-38);国家自然科学基金项目(30972369)作者简介:朱丽梅(1972—),副教授,博士。*吴小芹(通信作者),教授。 Email: xqwu@njfu.com.cn。引文格式:朱丽梅,吴小芹,徐旭凌. 具杀松材线虫活性的解淀粉芽孢杆菌JKJS3的培养条件[J]. 南京林业大学学报:自然科学版,2010,34(5):55-58.
更新日期/Last Update: 2010-10-08