[1]建徐超,吴小芹,林司曦,等.马尾松根部蛋白双向电泳分离体系的构[J].南京林业大学学报(自然科学版),2011,35(01):015-18.[doi:10.3969/j.jssn.1000-2006.2011.01.004]
 XU Chao,WU Xiaoqin,LIN Sixi,et al.Establishment of twodimensional gel electrophoresis system for analyzing the root protein of Pinus massoniana[J].Journal of Nanjing Forestry University(Natural Science Edition),2011,35(01):015-18.[doi:10.3969/j.jssn.1000-2006.2011.01.004]
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马尾松根部蛋白双向电泳分离体系的构
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
35
期数:
2011年01期
页码:
015-18
栏目:
研究论文
出版日期:
2011-01-01

文章信息/Info

Title:
Establishment of twodimensional gel electrophoresis system for analyzing the root protein of Pinus massoniana
作者:
建徐超吴小芹林司曦张红岩
南京林业大学,江苏省有害生物入侵预防与控制重点实验室,江苏南京210037
Author(s):
XU Chao WU Xiaoqin LIN Sixi ZHANG Hongyan
Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing 210037, China
关键词:
菌根 根部蛋白 双向电泳 马尾松
Keywords:
mycorrhiza root protein 2DE system Pinus massoniana
分类号:
S7184
DOI:
10.3969/j.jssn.1000-2006.2011.01.004
文献标志码:
A
摘要:
为了在蛋白质水平探讨外生菌根提高马尾松抗旱能力的机制,笔者通过对菌根化和非菌根化马尾松植株根部蛋白提取和SDS聚丙烯酰胺凝胶染色条件进行研究,建立了一套较为适合马尾松根部蛋白双向电泳分离的试验体系。结果表明:在每IPG胶条300 μg上样量条件下,采用酚抽法辅以适度超声波破碎进行马尾松根部蛋白提取、敏化和银染后水洗时间均为5 min×3次的快速银染程序进行染色,可以获得较为理想的马尾松根部蛋白双向电泳图谱,该方法可为其他松属树种的蛋白质组学等研究提供参考。
Abstract:
Plant vital activities could be revealed on protein level. Obtaining good twodimensional gel electrophoresis(2DE) profile is the precondition for experimental procedures such as mass spectrum analysis, and so on. In order to explore the mechanism of mycorrhiza underlying drought resistance of Pinus massoniana on protein level, a set of optimized experiment system of 2DE for analyzing root protein extracted from P.massoniana has been established. The ideal 2DE profile could be obtained when protein was extracted by phenol extraction and shattered the cells with ultrasonic crasher, and then the gels were stained by fast silver stain and washed three times for 5 min after sensitizing and dying. The suitable concentration of protein was 300 μg per IPG gel. It also provided reference for the proteomic studies on other pine species.

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备注/Memo

备注/Memo:
收稿日期:2009-12-29修回日期:2010-04-29基金项目:国家自然科学基金项目(30571471);“十一五”国家科技支撑计划(2006BAD08A1002)作者简介:徐超(1979—),博士。*吴小芹(通信作者),教授。Email: xqwu@njfu.com.cn。
更新日期/Last Update: 2011-01-14