[1]宋向阳,方浩,欧阳嘉,等.液态混合菌发酵优化纤维素酶组分[J].南京林业大学学报(自然科学版),2011,35(02):078-82.[doi:10.3969/j.jssn.1000-2006.2011.02.017]
 SONG Xiangyang,FANG Hao,OUYANG Jia,et al.ptimization of cellulase components by mixed culture of Trichoderma reesei and Aspergillus niger[J].Journal of Nanjing Forestry University(Natural Science Edition),2011,35(02):078-82.[doi:10.3969/j.jssn.1000-2006.2011.02.017]
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液态混合菌发酵优化纤维素酶组分
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
35
期数:
2011年02期
页码:
078-82
栏目:
研究论文
出版日期:
2011-03-28

文章信息/Info

Title:
ptimization of cellulase components by mixed culture of Trichoderma reesei and Aspergillus niger
作者:
宋向阳方浩欧阳嘉李鑫勇强
南京林业大学化学工程学院,林木遗传与生物技术省部共建教育部重点实验室,江苏南京210037
Author(s):
SONG XiangyangFANG HaoOUYANG JiaLI XinYONG Qiang
Key Laboratory of Forest Genetic and Biotechnology Ministry of Education, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037 China
关键词:
里氏木霉黑曲霉混合培养纤维素酶组分优化
Keywords:
Trichoderma reesei Aspergillus niger mixed culture cellulase component optimization
分类号:
TQ925
DOI:
10.3969/j.jssn.1000-2006.2011.02.017
文献标志码:
A
摘要:
在里氏木霉和黑曲霉液态混合条件下培养纤维素酶,分析两个菌种的接种比和延迟黑曲霉的接种时间对产酶的影响,探讨两个菌种发挥协同作用的最佳条件。结果表明:黑曲霉延迟接种48 h及里氏木霉与黑曲霉接种质量比1∶1时 ,所产纤维素酶的滤纸酶活为1163 μmol/(min·mL);β-葡萄糖苷酶活为0606 μmol/(min·mL),β-葡萄糖苷酶活与滤纸酶活比值为0521,比单一里氏木霉产纤维素酶的酶活高,并在后续的酶解效果对比中表现最佳。48 h时的酶解得率为6561 %,高于里氏木霉单一培养时所产纤维素酶的得率5391 %和商品纤维素酶的得率4964 %。说明通过混合发酵,纤维素酶的组分得到了优化。
Abstract:
Mixed culture of Trichoderma reesei and Aspergillus niger was investigated to optimize the cellulase components in liquidstate fermentation. A mode of delayed inoculation was applied for cocultivation of T. reesei and A. niger. After cultivation of T. reesei for 48 h, A. niger was inoculated at the 1∶1 inoculum ratio. The βglucosidase activity and filter paper activity of the mixed fungi culture were 0.606 μmol/(min·mL) and 1.163 μmol/(min·mL), respectively, corresponding to a ratio of 0.521, which was higher than that of T. reesei under single cultivation. The yield of enzymatic hydrolysis was 65.61 % by the cocultivated cellulase, which was higher than those of singlecultivated cellulase and commercial cellulase. These results demonstrated that cocultivation of T. reesei and A. niger was suitable for optimization of cellulase components.

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备注/Memo

备注/Memo:
收稿日期:2010-04-29修回日期:2010-12-09 基金项目:国家自然科学基金项目(30871992);江苏省科技支撑计划(BE2010732);江苏高校科技创新团队项目作者简介:宋向阳(1965—),副教授,博士。Email: xiangyangsong@hotmail.com。引文格式:宋向阳,方浩,欧阳嘉,等. 液态混合菌发酵优化纤维素酶组分[J]. 南京林业大学学报:自然科学版,2011,35(2):78-82.
更新日期/Last Update: 2011-04-13