[1]张金凤,方升佐*,尚旭岚,等.青钱柳幼胚愈伤组织的诱导[J].南京林业大学学报(自然科学版),2012,36(05):047-50.[doi:10.3969/j.jssn.1000-2006.2012.05.008]
 ZHANG Jinfeng,FANG Shengzuo*,SHANG Xulan,et al.Callus induction from young embryos of Cyclocarya paliurus[J].Journal of Nanjing Forestry University(Natural Science Edition),2012,36(05):047-50.[doi:10.3969/j.jssn.1000-2006.2012.05.008]
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青钱柳幼胚愈伤组织的诱导
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
36
期数:
2012年05期
页码:
047-50
栏目:
研究论文
出版日期:
2012-09-30

文章信息/Info

Title:
Callus induction from young embryos of Cyclocarya paliurus
作者:
张金凤方升佐*尚旭岚徐锡增
南京林业大学森林资源与环境学院,江苏南京210037
Author(s):
ZHANG JinfengFANG Shengzuo*SHANG XulanXU Xizeng
College of Forest Resources and Environment, Nanjing Forestry University,Nanjing 210037,China
关键词:
青钱柳 幼胚 愈伤组织 培养基 植物生长调节剂
Keywords:
Cyclocarya paliurus young embryo callus media plant growth regulator
分类号:
S722;Q944.5
DOI:
10.3969/j.jssn.1000-2006.2012.05.008
文献标志码:
A
摘要:
以青钱柳幼胚为外植体,研究了不同幼胚期、基本培养基种类、植物生长调节剂种类及其浓度配比对愈伤组织诱导的影响。结果表明,不同发育期的幼胚诱导出的愈伤组织在形态上差异较大,青钱柳种子发育至子叶胚中期较适合愈伤组织的诱导。青钱柳幼胚愈伤诱导时基本培养基以改良DKW效果最佳,诱导率达到了88.89 %,且无褐化现象;含2,4-D与KT组合的培养基较适宜愈伤组织的诱导,其中2,4-D(0.5 mg/L)+KT (2.0 mg/L)和2,4-D (1.0 mg/L)+KT (1.0 mg/L)的组合对青钱柳幼胚诱导愈伤组织效果最佳,诱导率分别为80.00 %和78.33 %。
Abstract:
Using young embryos of Cyclocarya paliurus as explants, we investigated the effects of embryo development period, basic media, types and the concentrations of plant growth regulators on callus induction of C. paliurus. The results showed that there were obvious differences in the shape of callus among different embryo development periods, and the middle cotyledon embryos were most suitable for callus induction. As a basic media, improved DKW medium was the best selection for young embryo callus induction, of which induction rate could hit to 88.89 % with no browning phenomenon. The medium containing 2,4-D and KT was more appropriate for callus induction. The 2,4-D (0.5 mg/L)+KT (2.0 mg/L) as well as 2,4-D (1.0 mg/L)+KT (1.0 mg/L) were optimal for callus induction of C. paliurus young embryo, and the induction rates were 80.00 % and 78.33 % respectively.

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备注/Memo

备注/Memo:
收稿日期:2011-11-17修回日期:2012-04-25
基金项目:江苏高校优势科技创新团队科研计划(苏教科[2009]10号);国家林业局林业科学技术推广项目﹙[2011]18号);国家林业公益性行业科研专项项目(200904046)
第一作者:张金凤,硕士生。*通信作者:方升佐,教授。E-mail: fangsz@njfu.edu.cn。
引文格式:张金凤,方升佐,尚旭岚,等. 青钱柳幼胚愈伤组织的诱导[J]. 南京林业大学学报:自然科学版,2012,36(5):47-50.
更新日期/Last Update: 2012-09-30