[1]官民晓,刘雪梅*,张 妍,等.白桦SPL8转录因子基因的分离及转录表达分析[J].南京林业大学学报(自然科学版),2013,37(03):017-22.[doi:10.3969/j.issn.1000-2006.2013.03.004]
　GUAN Minxiao,LIU Xuemei*,ZHANG Yan,et al.Isolation and transcription expression analysis of SPL8 transcription factors gene of Betula platyphylla[J].Journal of Nanjing Forestry University(Natural Science Edition),2013,37(03):017-22.[doi:10.3969/j.issn.1000-2006.2013.03.004]
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白桦SPL8转录因子基因的分离及转录表达分析/HTML
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
37
期数:
2013年03期
页码:
017-22
栏目:
研究论文
出版日期:
2013-05-20

文章信息/Info

Title:
Isolation and transcription expression analysis of SPL8 transcription factors gene of Betula platyphylla
作者:
官民晓; 刘雪梅*; 张 妍; 刘 瀛; 孙丰宾
东北林业大学生命科学学院,黑龙江 哈尔滨 150040
Author(s):
GUAN Minxiao;  LIU Xuemei*;  ZHANG Yan;  LIU Ying;  SUN Fengbin
College of Life Science,Northeastern Forest University, Harbin 150040, China
关键词:
白桦;  SPL8基因;  转录;  qRT-PCR;  突变体;  花发育;  差异表达
Keywords:
Betula platyphylla;  SPL8 gene;  transcript;  qRT-PCR;  mutant;  flower development;  differential expression
分类号:
Q943.2
DOI:
10.3969/j.issn.1000-2006.2013.03.004
摘要:
利用RACE和RT-PCR技术对白桦SPL转录因子SPL8进行克隆,获得cDNA全长为1 230 bp,其完整的开放读码框为930 bp,编码309个氨基酸,包含1个高度保守的SBP域,预测编码蛋白的分子质量为34.32 ku,理论等电点为9.08。将该基因命名为BplSPL8,并将它的cDNA序列提交到Genbank,登录号为JQ354964。同源序列比对分析表明,白桦BplSPL8与拟南芥AtSPL8、大豆GmSPL8、马铃薯ScSPL8、葡萄VvSPL8的氨基酸序列相似性分别为55.4%、73.1%、63.9%、70.8%。qRT-PCR结果显示,BplSPL8基因在不同组织均有表达,但在雄花序中最高,在种子和成熟花粉中最低; BplSPL8在雄花序不同发育阶段的表达量不同,另外,在雄花序发育突变体各组织中的表达水平均低于野生型。推测BplSPL8在白桦雄花序发育中的特定阶段起重要作用,其异常表达可能与雄性不育有关。
Abstract:
A 1 230 bp full-length cDNA of SPL8 gene was isolated from Betula platyphylla Suk. using RACE(rapid amplification of cDNA ends)and RT-PCR(reverse transcription polymerase chain reaction). SPL8 gene contains an open reading frame of 930 bp which encodes 309 amino acid with the deduced molecular mass around 34.32 ku and the theoretical isoelectric point of 9.08.The gene was named BplSPL8 and submitted to GenBank with accession number JQ354964.The homology of amino acid compared with Arabidopsis thaliana AtSPL8, Glycine max GmSPL8, Solanum chacoense ScSPL8 and Vitis vinifera VvSPL8 was 55.4%,73.1%,63.9% and 70.8%, respectively. The qRT-PCR result showed that BplSPL8 is expressed in all different tissues, the expression was the highest in the male inflorescences, whereas lowest in the seed and pollen. The expression level changed at different development stages in the male inflorescences. In addition, the expression levels of BplSPL8 in mutant was below that in wild-type in all different tissues, which suggests that BplSPL8 may play an important role on the development’s specific stage of the male inflorescences in the Betula and its abnormal expression may be associated with male sterility.

参考文献/References:

[1] Klein J, Saedler H, Huijser P. A new family of DNA binding proteins includes putative transcriptional regulators of the Antirrhinum majus floral meristem identity gene SQUAMOSA[J]. Mol Gen Genet, 1996, 250:7-16.
[2] Cardon G H, Hohmann S, Nettesheim K, et al. Functional analysis of the Arabidopsis thaliana SBP-box gene SPL3:a novel gene involved in the floral transition[J]. Plant J, 1997, 12:367-377.
[3] Lannenpaa M, Janonen I, Holtta-Vuori M, et al. A new SBP-box gene BpSPL1 in silver birch(Betula pendula)[J]. Plant Physiol,2004, 120:491-500.
[4] Becraft P W, Bongard-Pierce D K, Sylvester A W, et al. The LIGULELESS-1 gene acts tissue specifically in maize leaf development[J]. Dev Biol, 1990,141:220-232.
[5] Moreno M A, Harper L C, Krueger R W, et al. Liguleless1 encodes a nuclear-localized protein required for induction of ligules and auricles during maize leaf organogenesis[J].Genes Dev, 1997,11:616-628.
[6] Manning K, Tor M, Poole M, et al. A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening [J]. Nat Genet, 2006,38:948-952.
[7] 赵晓初,李贺,代红艳,等.草莓 miR156 靶基因 SPL9 的克隆与表达分析[J].中国农业科学,2011,44(12):2515-2522. Zhao X C, Li H, Dai H Y, et al. Cloning and expression analysis of miR156-targeted SPL9 gene from strawberry[J]. Scientia Agricultura Sinica, 2011,44(12):2515-2522.
[8] Wang Y, Hu Z, Yang Y, et al. Function annotation of an SBP-box gene in Arabidopsis based on analysis of co-expression networks and promoters[J]. Int J Mol Sci, 2009,10:116-132.
[9] Wu G, Poethig R S. Temporal regulation of shoot development in Arabidopsis thaliana by miR156 and its target SPL3[J]. Development, 2006,133:3539-3547.
[10] Nan Y, Cai W J, Wang S C, et al. Temporal control of trichome distribution by microRNA156-targeted SPL genes in Arabidopsis thaliana[J]. Plant Cell, 2011, http://dx.doi.org/10.1105/tpc.109.072579.
[11] Usami T, Horiguchi G, Yano S, et al. The more and smaller cells mutants of Arabidopsis thaliana identify novel roles for SQUAMOSA PROMOTER BINDING PROTEIN-LIKE genes in the control of heteroblasty [J]. Development, 2009,136,955-964.
[12] Wang H, Nussbaum-Wagler T, Li B, et al. The origin of the naked grains of maize[J]. Nature, 2005,436:714-719.
[13] Jiao Y, Wang Y, Xue D, et al. Regulation of OsSPL14 by OsmiR156 defi nes ideal plant architecture in rice [J]. Nat Genet, 2010,42:541-544.
[14] Unte U S, Sorensen A M, Pesaresi P, et al. SPL8, an SBP-box gene that affects pollen sac development in Arabidopsis[J]. Plant Cell, 2003,15:1009-1019.
[15] Zhang Y, Schwarz S, Saedler H, et al. SPL8,a local regulator in a subset of gibberellin-mediated developmental processes in Arabidopsis[J]. Plant Mol Biol, 2007,63:429-439.
[16] Eriksson M, Moseley J L, Tottey S, et al. Genetic dissection of nutritional copper signaling in chlamydomonas distinguishes regulatory and target genes[J]. Genetics, 2004,168:795-807.
[17] Stone J, Liang X, Nekl E, et al. Arabidopsis AtSPL14,a plant-specic SBP-domain transcription factor, participates in plant development and sensitivity to fumonisin B1[J]. Plant J, 2005, 41:744-754.
[18] 刘雪梅,杨传平.白桦雌雄花发育周期的时序特征[J].林业科学,2006,42(12):28-32. Liu X M, Yang C P. Temporal characteristics of developmental cycles of female and male flowers in Betula platyphylla in northeastern China[J]. Scientia Silvae Sinicae, 2006, 42(12):28-32.
[19] 刘雪梅,周菲,邢磊,等.白桦雄花突变体及其细胞学特征[J].东北林业大学学报,2010,38(6):1-3,14. Liu X M, Zhou F, Xing L, et al. Cytological characteristics of male floral mutantin Betula platyphylla[J]. Jouranl of Northeast Forestry University, 2010,38(6):1-3,14.
[20] 施季森.迎接21世纪现代林木生物技术育种的挑战[J].南京林业大学学报,2000,24(1):1-6. Shi J S. To meet the 21st century modern forest biotechnology breeding challenge[J].Journal of Nanjing Forestry Unviersity, 2000,24(1):1-6.
[21] 张妍,刘瀛,孙丰宾,等.白桦APETALA2(AP2)转录因子基因的分离及其表达[J].林业科学研究,2012,25(2):254-260. Zhang Y, Liu Y, Sun F B, et al. Isolation and expression of APETALA2 transcription factor gene in Betula platyphylla[J]. Forest Research, 2012,25(2):254-260.
[22] 曾凡锁,南楠,詹亚光.富含多糖和次生代谢产物的白桦成熟叶中总RNA的提取[J].植物生理学通讯,2007,43(5):913-916. Zeng F S, Nan N, Zhan Y G. Extraction of total RNA from mature leaves rich in polysaccharides and secondary metabolites of Betula platyphylla Suk.[J].Plant Physiology Communications, 2007,43(5):913-916.
[23] 戴超,刘雪梅,周菲.白桦基因表达半定量RT-PCR中内参基因的选择[J].经济林研究,2011,29(1):34-39. Dai C, Liu X M, Zhou F. Selection of internal control genes in semi-quantitative RT-PCR in Betula platyphylla[J].Economic Forest Researches, 2011,29(1):34-39.
[24] Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T))[J]. Methods Methods, 2001,25:402-408.
[25] Yamasaki K, Kigawa T, Inoue M, et al. A novel zinc-binding motif revealed by solution structures of DNA-binding domains of Arabidopsis SBP-family transcription factors[J]. J Mol Biol, 2004,337(1):49-63.
[26] Birkenbihl R P, Jach G, Saedler H, et al. Functional dissection of the plant-specific SBP-domain, overlap of the DNA-binding and nuclear localization domains [J]. J Mol Biol, 2005,352:585-596.
[27] 吴晓宇,胡尚连,曹颖,等.慈竹 CCoAOMT 基因的克隆及生物信息学分析[J].南京林业大学学报:自然科学版,2012,36(3):17-22. Wu X Y, Hu S L, Cao Y, et al. Cloning of CCoAOMT gene in Neosinocalamus affinis and its bioinformatics analysis[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2012,36(3): 17-22.
[28] 张萍,潘惠新,黄敏仁,等.杨树抗杨四瘿螨相关基因的表达分析[J].南京林业大学学报:自然科学版, 2009, 33(4):8-12. Zhang P, Pan H X, Huang M R, et al. Differentially expressed gene analysis of populus induced by Tetra lobulifera(Keifer)[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2009, 33(4): 8-12.
[29] Yang J H, Fang Z M, Yu J Q. Relationship between cytoplasmic male sterility and SPL-like gene expression in stem mustard [J]. Physiol Plantarum, 2008,133:426-434.

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备注/Memo

备注/Memo:
收稿日期:2012-03-18 修回日期:2012-06-23
基金项目:国家自然科学基金项目(31100449); 黑龙江省自然科学基金项目(C201040); 东北林业大学研究生论文资助项目(ST1P10)
第一作者:官民晓,硕士生。*通信作者:刘雪梅,副教授,博士。liuxuemei@nefu.edu.cn。
引文格式:官民晓,刘雪梅,张妍,等. 白桦SPL8转录因子基因的分离及转录表达分析[J]. 南京林业大学学报:自然科学版,2013,37(3):17-22.
更新日期/Last Update: 2013-05-31