[1]房小雪,郑 楠,赵 敏*.新月弯孢霉高产漆酶菌株的诱变及其培养条件优化[J].南京林业大学学报(自然科学版),2014,38(02):117-122.[doi:10.3969/j.issn.1000-2006.2014.02.022]
 FANG Xiaoxue,ZHENG Nan,ZHAO Min*.Mutation and culture conditions optimization of Curvularia lunata forhigh producing strain of laccase[J].Journal of Nanjing Forestry University(Natural Science Edition),2014,38(02):117-122.[doi:10.3969/j.issn.1000-2006.2014.02.022]
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新月弯孢霉高产漆酶菌株的诱变及其培养条件优化
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
38
期数:
2014年02期
页码:
117-122
栏目:
研究论文
出版日期:
2014-03-24

文章信息/Info

Title:
Mutation and culture conditions optimization of Curvularia lunata for high producing strain of laccase
文章编号:
1000-2006(2014)02-0117-06
作者:
房小雪郑 楠赵 敏*
东北林业大学生命科学学院,黑龙江 哈尔滨 150040
Author(s):
FANG XiaoxueZHENG NanZHAO Min*
College of Life Science, Northeast Forestry University, Harbin 150040, China
关键词:
新月弯孢霉 诱变 漆酶 优化条件
Keywords:
Curvularia lunata mutagenesis laccase optimization
分类号:
Q939.9
DOI:
10.3969/j.issn.1000-2006.2014.02.022
文献标志码:
A
摘要:
利用紫外线-60Co-γ、微波及硫酸二乙酯诱变新月弯孢霉菌株,使其产漆酶能力大幅度提高。漆酶活性由原来的0.55 μmol/(min·mL)提高到1.949 μmol/(min·mL)。进一步通过单因素实验及正交实验得出最佳产酶条件为:发酵温度30 ℃,发酵时间72 h,培养基pH为 6,装液量120 mL,摇床转速160 r/min,土豆200 g/L,蔗糖30 g/L,大豆蛋白胨5 g/L,KH2PO4 5 g/L,愈创木酚0.2 mmol/L,吐温80 0.1 mL/L,Mg2+ 0.5 mmol/L,Cu2+ 1 mmol/L,Ca2+ 1 mmol/L,Mn2+ 0.25 mmol/L。在此条件下,最终漆酶活性可达2.306 μmol/(min·mL)。
Abstract:
In this paper,Curvularia lunata strains were mutagenized by UV-60Co-γ,microwave and diethyl sulfate. By means of the mutation treatment, the laccase ability of C. lunata substantially increased from 0.55 μmol/(min·mL)to 1.949 μmol/(min·mL). In order to increase the laccase ability further, the single factor experiment and orthogonal design experiment were carried out, the result indicated that the optimal conditions were as follows: fermentation temperature 30 ℃, fermentation time 72 h, culture medium pH 6, liquid volume 120 mL, rotation rate 160 r/min, potato 200 g/L, sucrose 30 g/L, soy peptone 5 g/L, KH2PO4 5 g/L, guaiacol 0.2 mmol/L, Tween 80 0.1 mL/L, Mg2+ 0.5 mmol/L, Cu2+ 1 mmol/L, Ca2+ 1 mmol/L, Mn2+ 0.25 mmol/L. Ultimate laccase activity 2.306 μmol/(min·mL).

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2013-06-26 修回日期:2013-12-20
基金项目:国家林业局“948”项目(2012-4-03); 国家自然科学基金项目(31170553)
第一作者:房小雪,硕士生; 郑楠,博士生。*通信作者:赵敏,教授。E-mail: 82191513@163.com。
引文格式:房小雪,郑楠,赵敏. 新月弯孢霉高产漆酶菌株的诱变及其培养条件优化[J]. 南京林业大学学报:自然科学版,2014,38(2):117-122.
更新日期/Last Update: 2014-03-10