[1]李 祯,伊贤贵,顾 宇,等.山樱花基因组大小的测定[J].南京林业大学学报(自然科学版),2014,38(增刊):017-19.[doi:10.3969/j.issn.1000-2006.2014.S1.004]
 LI Zhen,YI Xiangui,GU Yu,et al.The genome size determination of Cerasus serrulata[J].Journal of Nanjing Forestry University(Natural Science Edition),2014,38(增刊):017-19.[doi:10.3969/j.issn.1000-2006.2014.S1.004]
点击复制

山樱花基因组大小的测定
分享到:

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
38
期数:
2014年增刊
页码:
017-19
栏目:
研究论文
出版日期:
2014-10-29

文章信息/Info

Title:
The genome size determination of Cerasus serrulata
文章编号:
1000-2006(2014)S1-0017-03
作者:
李 祯伊贤贵顾 宇王贤荣*
南京林业大学生物与环境学院,江苏 南京 210037
Author(s):
LI Zhen YI Xiangui GU Yu WANG Xianrong*
College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China
关键词:
山樱花 流式细胞仪 基因组大小
Keywords:
Cerasus serrulata FCM genome size
分类号:
S685
DOI:
10.3969/j.issn.1000-2006.2014.S1.004
文献标志码:
A
摘要:
为了测定山樱花基因组的大小,获得适合山樱花裂解的流式细胞术的最适裂解液,优化了樱属植物流式细胞术流程。结果显示,在所对比的6种裂解液中,LB01最适合樱属植物细胞裂解。以大豆(Glycine max (L.)Merrill)为内标,比较待测样品和内参峰值,计算得出山樱花基因组大小为366.67 Mb,C值为0.375 pg。可用于樱属植物的倍性鉴定。
Abstract:
The present study optimized the process of flow cytometry(FCM)for Cerasus with the purpose of determining the genome size of Cerasus serrulata and obtaining optimal buffer for its FCM process. The results showed that the LB01 was the best buffer for C. serrulata among the six analyzed buffers. This paper used Clycine max(L.)Merrill was the internal standard to test the genome size of C. serrulata, which was 366.67 Mb with C value 0.375 pg. That present results would lay foundations for ploidy identification of Cerasus serrulata.

参考文献/References:

[1] 李潞滨, 武静宇, 胡陶, 等. 毛竹基因组大小测定[J]. 植物学通报, 2008, 25(5): 547-578.Li L B, Wu J Y, Hu T, et al. Estimation of genome size of moso bamboo[J]. Chinese Bulletin of Botany, 2008, 25(5): 74-578.
[2] Bennett M D, Leitch I J. Genome size evolution in plants[J]. The Evolution of the Genome, 2005: 89-162.
[3] Gregory T R. Genome size evolution in animals[G]//Gregory T R. The Evolution of the Genome. San Diego: Elsevier, 2005:3-87.
[4] Greilhuber J, Dolezel M Lysak, Bennett M D. The origin evolution and proposed stabilization of the terms genome size and C-value to describe nuclear DNA contents[J]. Annals of Botany, 2005, 95(1): 225-260.
[5] 李蒙. 山樱花高海拔居群生态学特征及组织培养[D]. 南京:南京林业大学, 2013.Li M. Ecological characteristics and tissue culture of the high altitude population of Cerasus serrulata[D]. Nanjing:Nanjing Forestry University, 2013.
[6] 陈瑞阳. 中国主要经济植物基因组染色体图谱[M]. 北京: 科学出版社, 2003.
[7] Oginuma K. Karyomorphological studies on Prunus in Japan[J]. Journal of Science of the Hiroshima University:Series B, Division 2. Botany, 1987, 2(21): 1-66.
[8] Oginuma K. Karyomorphological studies on some species of Japanese Prunus[C]//Proc Sino-Jpn Symposium Pl Chromes. Plant Chromosome Research, 1987:131-134.
[9] 田新民, 周香艳, 弓娜. 流式细胞术在植物学研究中的应用——检测植物核DNA含量和倍性水平[J]. 中国农学通报, 2011, 9: 21-27.Tian X M, Zhou X Y, Gong N, et al. Application of flow cytometry in plant research——Analysis of nuclear DNA content and ploidy level in plant cell[J]. Chinese Agricultural Science Bulletin,2011, 9: 21-27.
[10] Doleel J, Greilhuber J, Suda J. Estimation of nuclear DNA content in plants using flow cytometry[J]. Nature Protocols, 2007, 2(9): 2233-2244.
[11] Loureiro J, Rodriguez E, Doleel J, et al. Two new nuclear isolation buffers for plant DNA flow cytometry: a test with 37 species[J]. Annals of Botany, 2007, 100(4): 875-888.
[12] Courtney H Wilcox. 美科学家完成大豆基因组测序[J]. 农业生物技术学报, 2010(1): 191.Courtney H Wilcox. US Scientist sequenced the genome of soybean[J]. Journal of Agricultural Biotechnology, 2010(1): 191.
[13] Johnson J S, Bennett M D, Rayburn A L, et al. Reference standards for determination of DNA content of plant nuclei[J]. American Journal of Botany, 1998, 82: 829-833.

相似文献/References:

[1]李蒙,严邦祥,赵昌高,等.大仰山高山湿地山樱花种群数量结构特征[J].南京林业大学学报(自然科学版),2013,37(05):040.[doi:10.3969/j.issn.1000-2006.2013.05.008]
 LI Meng,YAN Bangxiang,ZHAO Changgao,et al.Numeric and structural characteristics of Cerasus serrulata population aroundthe highelevation wetlands of Dayangshan[J].Journal of Nanjing Forestry University(Natural Science Edition),2013,37(增刊):040.[doi:10.3969/j.issn.1000-2006.2013.05.008]
[2]李 蒙,伊贤贵,王华辰,等.山樱花地理分布与水热环境因子的关系[J].南京林业大学学报(自然科学版),2014,38(增刊):074.[doi:10.3969/j.issn.1000-2006.2014.S1.016]
 LI Meng,YI Xiangui,WANG Huachen,et al.Studies on the relationship between Cerasus serrulata distribution region and the environmental factors[J].Journal of Nanjing Forestry University(Natural Science Edition),2014,38(增刊):074.[doi:10.3969/j.issn.1000-2006.2014.S1.016]
[3]伊贤贵,陈 洁,尤禄祥,等.山樱花群体遗传多样性的SSR分析[J].南京林业大学学报(自然科学版),2018,42(05):025.[doi:10.3969/j.issn.1000-2006.201702036]
 YI Xiangui,CHEN Jie,YOU Luxiang,et al.Genetic divetsity of Cerasus serrulata populations assessed by SSR markers[J].Journal of Nanjing Forestry University(Natural Science Edition),2018,42(增刊):025.[doi:10.3969/j.issn.1000-2006.201702036]

备注/Memo

备注/Memo:
收稿日期:2014-09-10
基金项目:江苏省科技支撑计划(BE2012346); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:李祯,硕士生*通信作者:王贤荣,教授,博士。E-mail: wangxianrong66@njfu.edu.cn。
引文格式:李祯,伊贤贵,顾宇,等. 山樱花基因组大小的测定[J]. 南京林业大学学报:自然科学版,2014,38(S1):17-19.
更新日期/Last Update: 1900-01-01