[1]历 娜,栗 君,卢 磊,等.固定化漆酶在染料脱色中的应用[J].南京林业大学学报(自然科学版),2014,38(05):129-133.[doi:10.3969/j.issn.1000-2006.2014.05.025]
 LI Na,LI Jun,LU Lei,et al.Application of immobilized laccase in dye decolorization[J].Journal of Nanjing Forestry University(Natural Science Edition),2014,38(05):129-133.[doi:10.3969/j.issn.1000-2006.2014.05.025]
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固定化漆酶在染料脱色中的应用
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
38
期数:
2014年05期
页码:
129-133
栏目:
研究论文
出版日期:
2014-09-30

文章信息/Info

Title:
Application of immobilized laccase in dye decolorization
文章编号:
1000-2006(2014)05-0129-05
作者:
历 娜栗 君卢 磊王靖瑶王天女李国富徐腾飞赵 敏*
东北林业大学生命科学学院,黑龙江 哈尔滨 150040
Author(s):
LI Na LI Jun LU Lei WANG Jingyao WANG Tiannü LI Guofu XU Tengfei ZHAO Min*
College of Life Sciences, Northeast Forestry University, Harbin 150040, China
关键词:
解淀粉芽孢杆菌 固定化漆酶-介体系统 染料脱色
Keywords:
Bacillus amyloliquefaciens immobilized laccase-mediator dye decolorization
分类号:
Q814.9
DOI:
10.3969/j.issn.1000-2006.2014.05.025
文献标志码:
A
摘要:
为了使解淀粉芽孢杆菌LC03的芽孢漆酶对染料废水有更好的脱色作用,将其固定于海藻酸钙的凝胶中,测定其最适温度和最适pH及操作稳定性,并对RBBR、靛红(IC)、活性黑(RB5)、结晶紫(CV)等4种单染料进行脱色; 通过加入漆酶介体,研究固定化漆酶-介体系统对单染料及模拟染料废水脱色的影响。结果表明:固定化漆酶最适温度65 ℃,最适pH为6.2,重复利用10次酶活还在80%以上; 固定化小球对染料CV脱色作用尤为明显,脱色1 h后脱色率就达到80%以上,对其他3种染料也有一定的脱色作用; 漆酶介体对染料的脱色均有促进作用,对染料IC、RBBR和RB5的促进作用尤为明显,且随着介体浓度的升高脱色率增大; 固定化小球提高了漆酶的重复利用率,增加了漆酶的稳定性,在酸性、碱性、中性条件下,对单染料和模拟染料废水都有很好的脱色效果。
Abstract:
In order to obtain a good decolorization efficiency of dye wastewater,we fixed spore laccase from Bacillus amyloliquefaciens LC03 in seaweed acid calcium gel and determined its optimum temperature and pH and its operational stability.Then we studied the decolorization of four single dyes,including remazol brilliant blue R(RBBR),reactive black 5,indigo carmine and crystal violet, and investigated the effects of immobilized laccase-mediators with laccase mediators added on single dye to stimulate dye wastewater.Experiments showed that the optimum temperature was 65 ℃,the optimum pH was 6.2 and after 10 cycles of operation the immobilized enzyme remained over 80% of its initial activity. The immobilized pellets had a significant effect on decolorization of CV and more than 80% of CV was decolorized after 1 hour,while it also played some role in the decolorization of other three single dyes. Laccase mediators had a promoting effect on the decolorization of dyes especially on the IC,RBBR and RB5 and with the mediators concentration rising,the rate of decolorization increased. The immobilized pellets enhanced laccase repeated utilization ratio and increased the stability of laccase. And under the acid, alkaline, and neutral conditions, immobilizod laccase mediators had a good decolorizing effect on single dye and simulated dye wastewater which indicating potential application of this immobilized laccase-mediators in dye wastewater treatment.

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备注/Memo

备注/Memo:
收稿日期:2013-10-08 修回日期:2014-04-23
基金项目:国家自然科学基金项目(31170553,30671702,30170775); 国家林业局“948”项目(2012-4-03)
第一作者:历娜,硕士生; 栗君,硕士生。*通信作者:赵敏,教授。E-mail: 82191513@163.com。
引文格式:历娜,栗君,卢磊,等. 固定化漆酶在染料脱色中的应用[J]. 南京林业大学学报:自然科学版,2014,38(5):129-133.
更新日期/Last Update: 2014-10-31