[1]周文才,侯 静,郭 炜,等.基于SSR标记的美洲黑杨杂交子代的鉴定[J].南京林业大学学报(自然科学版),2015,39(03):045-49.[doi:10.3969/j.issn.1000-2006.2015.03.009]
 ZHOU Wencai,HOU Jing,GUO Wei,et al.Identification of the true hybrids for Populus deltoides by using SSR markers[J].Journal of Nanjing Forestry University(Natural Science Edition),2015,39(03):045-49.[doi:10.3969/j.issn.1000-2006.2015.03.009]
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基于SSR标记的美洲黑杨杂交子代的鉴定/HTML
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
39
期数:
2015年03期
页码:
045-49
栏目:
研究论文
出版日期:
2015-05-30

文章信息/Info

Title:
Identification of the true hybrids for Populus deltoides by using SSR markers
文章编号:
1000-2006(2015)03-0045-05
作者:
周文才12 侯 静1 郭 炜1 陈赢男1万志兵1 尹佟明1*
1.南京林业大学林学院,江苏 南京 210037;
2.江西省林业科学院,江西 南昌 330032
Author(s):
ZHOU Wencai12 HOU Jing1 GUO Wei1 CHEN Yingnan1 WAN Zhibing1 YIN Tongming1*
1. College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
2. Jiangxi Academy of Forestry, Nanchang 330032, China
关键词:
美洲黑杨 子代鉴定 SSR标记 遗传图谱构建
Keywords:
Populus deltoides hybrids identification SSR markers genetic map construction
分类号:
S722; Q78
DOI:
10.3969/j.issn.1000-2006.2015.03.009
文献标志码:
A
摘要:
杂交子代的纯度直接影响到遗传图谱的质量,因而在遗传图谱构建前要对杂交子代进行鉴定与选择。笔者从300对SSR标记中,筛选出11对能明确显示亲本来源的标记,利用这11对标记对380株美洲黑杨杂交子代进行了鉴定。结果表明:有372个个体因同时具有亲本各1条等位基因而被认定为亲子代可用于图谱构建,其余8个个体因产生了非亲本来源或额外的等位基因,这些个体可能源于花粉污染或染色体异常,在作图过程中宜从作图群体中排除。
Abstract:
Since the purity of hybrids affects the quality of the genetic map, identification and selection of hybrids should be done before the genetic map construction. In this study, we screened 11 SSR pairs primer that could be used to track the kinship of the hybrids from a total 300 pairs primer. Subsequently, these primers were used to test 380 offsprings from a hybridization of Populus deltoides. Among which, 372 individuals were revealed to own complementary alleles for each parent, and they were identified as true hybrids for genetic map construction. Whereas the remaining eight individuals, they were observed to own non-parental alleles or extra parental alleles, suggesting these progeny were either resulted from pollen contamination or chromosomal abnormalities. Thus they were excluded from map construction.

参考文献/References:

[1] 何祯祥,施季森,邱进清,等.林木遗传图谱构建的技术与策略[J].浙江林学院学报,1998, 15(2):151-157.He Z X, Shi J S, Qiu J Q, et al. Technique and strategy of genome mapping in forest trees[J]. Journal of Zhejiang Forestry College, 1998, 15(2): 151-157.
[2] Frewen B E, Chen T H H, Howe G T, et al. Quantitative trait loci and candidate gene mapping of bud set and bud flush in Populus[J]. Genetics, 2000, 154(2): 837-845.
[3] Cervera M T, Storme V, Ivens B, et al. Dense genetic linkage maps of three Populus species(Populus deltoides, P. nigra and P. trichocarpa)based on AFLP and microsatellite markers[J]. Genetics, 2001, 158(2): 787-809.
[4] 李博.毛白杨与毛新杨转录组图谱构建及若干性状的遗传学联合分析[D].北京:北京林业大学,2009.Li B. Construction of transcriptome maps and QTL analyses of some complex traits by integrating genomics and transcriptomics for Populus tomentosa Carr. and P.tomentos×P.bolleana[D]. Beijing: Beijing Forestry University, 2009.
[5] 董维,苏舒,游录鹏,等.高粱F6代群体分蘖数的QTL定位[J].南京林业大学学报:自然科学版,2013,37(2): 55-58.Dong W, Su S, You L P, et al. QTLs analysis of tillers number in F6 sorghum population[J]. Journal of Nanjing Forestry University: Natural Sciences Edition, 2013, 2013,37(2):55-58.
[6] Yin T M, DiFazio S P, Gunter L E, et al. Large-scale heterospecific segregation distortion in Populus revealed by a dense genetic map[J]. Theoretical and Applied genetics, 2004, 109(3):451-463.
[7] Harald H, Goring H, Terwilliger J D. Linkage analysis in the presence of errors II: marker-locus genotyping errors modeled with hypercomplex recombination fractions[J]. American Journal of Human Genetics, 2000, 66(3): 1107-1118.
[8] Brzustowicz L M, Merette C, Xie X, et al. Molecular and statistical approaches to the detection and correction of errors in genotype databases[J]. American Journal of Human Genetics, 1993, 53(5): 1137.
[9] Lincoln S E, Lander E S. Systematic detection of errors in genetic linkage data[J]. Genomics, 1992, 14(3): 604-610.
[10] Abecasis G R, Cherny S S, Cardon L R. The impact of genotyping error on family-based analysis of quantitative traits[J]. European Journal of Human Genetics, 2001, 9(2): 130-134.
[11] Powell W, Machray G C, Provan J. Polymorphism revealed by simple sequence repeats[J]. Trends in Plant Science, 1996,1(7): 215-222.
[12] Maliepaard C, Jansen J, Van Ooijen J W. Linkage analysis in a full-sib family of an outbreeding plant species: overview and consequences for applications[J]. Genetics Research, 1997, 70(3): 237-250.
[13] Sundaram R M, Naveenkumar B, Biradar S K, et al. Identification of informative SSR markers capable of distinguishing hybrid rice parental lines and their utilization in seed purity assessment[J]. Euphytica, 2008, 163(2): 215-224.
[14] Xin Y Y, Zhang Z, Xiong Y P, et al. Identification and purity test of super hybrid rice with SSR molecular markers[J]. Rice Science, 2005, 12(1): 7-12.
[15] 王立新,常利芳,李宏博,等.小麦种子纯度的分子标记检测方法[J].麦类作物学报,2009,29(1):1-8.Wang L X, Chang L F, Li H B, et al. Method of wheat seeds purity testing by molecular markers[J]. Journal of Triticeae Crops, 2009, 29(1): 1-8.
[16] 吴明生,贾希海,田雷,等.利用SSR技术快速准确鉴定杂交玉米种子纯度[J].分子植物育种,2006,4(3):381-384.Wu M S, Jia X H, Tian L, et al. Rapid and reliable purity identification of F1 hybrids ofmaize(Zea may L.)using SSR markers[J]. Molecular Plant Breeding, 2006, 4(3)381-384.
[17] 王斯琪,唐诗哲,孔德仓,等.利用SSR标记进行枣树子代苗父本鉴定[J].园艺学报,2012,39(11):2133-2141.Wang S Q,Tang S Z,Kong D C, et al. Application of SSR markers for the identification of paternal parent for the seedlings of Chinese jujube[J]. Acta Horticulturae Sinica, 2012, 39(11): 2133-2141.
[18] 林二培,马海泉,樊民亮,等.百山祖冷杉SSR体系的建立及人工辅助授粉子代的初步鉴定[J].浙江农林大学学报,2011,28(2):234-240.Lin E P, Ma H Q, Fan M L, et al.An SSR reaction system for Abies beshanzuensis progeny with artificial pollination and progeny identification[J]. Journal of Zhejiang A&F University, 2011, 28(2): 234-240.
[19] 张红莲,李火根,胥猛,等.鹅掌楸属种及杂种的SSR分子鉴定[J].林业科学,2010,46(1):36-39.Zhang H L, Li H G, Xu M, et al. Identification of Liriodendron tulipifera, Liriodendron chinense and Hybrid Liriodendron using species-specific SSR markers[J].Scientia Silvae Sinicae, 2010,46(1):36-39.
[20] Tuskan G A, Gunter L E, Yang Z K, et al. Characterization of microsatellites revealed by genomic sequencing of Populus trichocarpa[J]. Canadian Journal of Forest Research, 2004, 34(1): 85-93.
[21] Yin T M, Zhang X Y, Gunter L E, et al. Microsatellite primer resource for Populus developed from the mapped sequence scaffolds of the Nisqually-1 genome[J]. New Phytologist, 2009, 181(2): 498-503.
[22] 房经贵,章镇,马正强,等.AFLP标记在两个芒果品种间杂交F1代的多态性及分离方式[J].中国农业科学,2000,33(3):19-24.Fang J G, Zhang Z, Ma Z Q, et al.The polymorphism and segregation patterns of AFLP markers in the F1 progenies from the cross of two Mango cultivars[J]. Scientia Agricultura Sinica, 2000,33(3):19-24.
[23] Moriya S, Iwanami H, Okada K, et al. A practical method for apple cultivar identification and parent-offspring analysis using simple sequence repeat markers[J]. Euphytica, 2011, 177(1): 135-150.
[24] Bradshaw Jr H D, Stettler R F. Molecular genetics of growth and development in Populus. I. Triploidy in hybrid poplars[J]. Theoretical and Applied Genetics, 1993, 86(2-3): 301-307.
[25] Kong F, Liu J, Chen Y, et al. Marker-aided selection of polyploid poplars[J]. BioEnergy Research, 2013, 6(3): 984-990.

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备注/Memo

备注/Memo:
收稿日期:2014-07-24 修回日期:2015-01-19
基金项目:国家林业公益性行业科研重大项目(201304102); 国家自然科学基金项目(31400564,31400563); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:周文才,博士生。*通信作者:尹佟明,教授。E-mail: tmyin@njfu.com.cn
引文格式:周文才,侯静,郭炜,等. 基于SSR标记的美洲黑杨杂交子代的鉴定[J]. 南京林业大学学报:自然科学版,2015,39(3):45-49.
更新日期/Last Update: 2015-05-30