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 ZHOU Wencai,HOU Jing,GUO Wei,et al.Identification of the true hybrids for Populus deltoides by using SSR markers[J].Journal of Nanjing Forestry University(Natural Science Edition),2015,39(03):045-49.[doi:10.3969/j.issn.1000-2006.2015.03.009]





Identification of the true hybrids for Populus deltoides by using SSR markers
周文才12 侯 静1 郭 炜1 陈赢男1万志兵1 尹佟明1*
1.南京林业大学林学院,江苏 南京 210037;
2.江西省林业科学院,江西 南昌 330032
ZHOU Wencai12 HOU Jing1 GUO Wei1 CHEN Yingnan1 WAN Zhibing1 YIN Tongming1*
1. College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
2. Jiangxi Academy of Forestry, Nanchang 330032, China
美洲黑杨 子代鉴定 SSR标记 遗传图谱构建
Populus deltoides hybrids identification SSR markers genetic map construction
S722; Q78
Since the purity of hybrids affects the quality of the genetic map, identification and selection of hybrids should be done before the genetic map construction. In this study, we screened 11 SSR pairs primer that could be used to track the kinship of the hybrids from a total 300 pairs primer. Subsequently, these primers were used to test 380 offsprings from a hybridization of Populus deltoides. Among which, 372 individuals were revealed to own complementary alleles for each parent, and they were identified as true hybrids for genetic map construction. Whereas the remaining eight individuals, they were observed to own non-parental alleles or extra parental alleles, suggesting these progeny were either resulted from pollen contamination or chromosomal abnormalities. Thus they were excluded from map construction.


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收稿日期:2014-07-24 修回日期:2015-01-19
基金项目:国家林业公益性行业科研重大项目(201304102); 国家自然科学基金项目(31400564,31400563); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:周文才,博士生。*通信作者:尹佟明,教授。E-mail: tmyin@njfu.com.cn
引文格式:周文才,侯静,郭炜,等. 基于SSR标记的美洲黑杨杂交子代的鉴定[J]. 南京林业大学学报:自然科学版,2015,39(3):45-49.
更新日期/Last Update: 2015-05-30