[1]杨 柳,方崇荣,张 建,等.气质联用鉴别降香黄檀与越南香枝的研究[J].南京林业大学学报(自然科学版),2016,40(01):097-103.[doi:10.3969/j.issn.1000-2006.2016.01.016]
 YANG Liu,FANG Chongrong,ZHANG Jian,et al.The identification of Dalbergia odorifera and D. tonkinensi by gas chromatography mass spectrometry[J].Journal of Nanjing Forestry University(Natural Science Edition),2016,40(01):097-103.[doi:10.3969/j.issn.1000-2006.2016.01.016]
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气质联用鉴别降香黄檀与越南香枝的研究
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
40
期数:
2016年01期
页码:
097-103
栏目:
研究论文
出版日期:
2016-01-31

文章信息/Info

Title:
The identification of Dalbergia odorifera and D. tonkinensi by gas chromatography mass spectrometry
文章编号:
1000-2006(2016)01-0097-07
作者:
杨 柳1方崇荣1张 建2于海霞1王 增1朱杰丽1杨伟明1柴振林1
1.浙江省林产品质量检测站,浙江 杭州 310023;
2.浙江省林业科学研究院,浙江省竹类研究 重点实验室,浙江 杭州 310023
Author(s):
YANG Liu1FANG Chongrong1ZHANG Jian2YU Haixia1WANG Zeng1 ZHU Jieli1YANG Weiming1CHAI Zhenlin1
1.Zhejiang Forestry Product Testing Station, Hangzhou 310023, China;
2.Zhejiang Provincial Key Lab for Bamboo Research,Zhejiang Forestry Academy,Hangzhou 310023,China
关键词:
降香黄檀 越南香枝 气质联用 可挥发性成分 脂溶性成分 材种鉴定
Keywords:
Dalbergia odorifera Dalbergia tonkinensi gas-chromatography-mass-spectrometry volatile components fat-soluble ingredients identification
分类号:
S781
DOI:
10.3969/j.issn.1000-2006.2016.01.016
文献标志码:
A
摘要:
为了研究降香黄檀与越南香枝木材中的化学成分,采用顶空/气质联用法测定了降香黄檀与越南香枝可挥发性成分与脂溶性成分,通过对指纹图谱的对比分析,得出一种可以准确区分两种材质的鉴别方法。结果表明:在可挥发性成分测定中,顶空进样器平衡温度是影响可挥发性成分检出种类及量的主要因素之一,可挥发性成分检出种类及量随平衡温度增高而增加; 两材种可挥发性成分较多,均达30种以上,且种类相似,虽含量有差别但不足以构成其特征性差异,无法将两材种加以区分。在脂溶性成分测定中,乙酸乙酯是提取两材种脂溶性成分的最佳试剂,石油醚提取效果较差,甲醇提取液对检测系统污染程度较高; 两材种脂溶性成分丰富、相同成分众多,且差异性成分也多。两材种均含大量的橙花叔醇、反式-橙花叔醇和6,7-环氧-蛇麻烯等3种脂溶性成分,三者之和均可达脂溶性成分总量的40%以上。越南香枝中有5种含量较高的脂溶性成分即3,3',4,4'-四甲氧基二苯乙烯、松属素、3-(2,4-二甲氧基苯基)-7-甲氧基苯并二氢吡喃、3,4-二氢-7-甲氧基-3-(2,3,4-三甲氧基苯基)-2H-1-苯并吡喃、N-[2-(3,4-二甲氧基苯基)乙基]-3-甲氧基-苯甲酰胺,这5种脂溶性成分在越南香枝中含量之和占总脂溶性成分的20%~40%,而在降香黄檀中不足1%,由此可将两材种进行有效鉴别和区分。分析认为,降香黄檀与越南香枝两材种的化学成分差异显著,其中可挥发性成分差异不明显,无法以此准确区分材种,而脂溶性成分种类及含量差异显著,可准确区分两材种。
Abstract:
For identifing Dalbergia odorifera and D. tonkinensi accurately, the volatile components and fat-soluble ingredients of D. odorifera and D. tonkinensi are tested with gGC-MS. In detecting volatile components, equilibrium temperature of headspace sampler was one of the main factors that the volatile substance species and quantity increased with increasing of the equilibrium temperature. These two wood species share more than 30 kinds of volatile components with great similarity but little difference in content, which was not characteristic for identification.In detecting fat-soluble ingredients ethyl acetate was the best extraction reagent. Both wood species occupied many similar and different fat-soluble ingredients. Similar ingredients included large amounts of 3,7,11-trimethyl-1,6,10-dodecatrien-3-ol,(6Z)3,7,11-trimethyl-1,6,10-dodecatrien-3-ol and 6,7-epoxide humulene(6Z), which accounted for more than 40% of the total fat-soluble ingredients.D. tonkinensi occupies 5 ingredients with higher content which amount up to 20%-40% of total fat-soluble substance, namely, 3,3',4,4'-tetra-methoxystilbene, 5,7-dihydroxyflavanone, 3-(2,4-dimethoxyphenyl)-7-methoxychroman, 3,4-dihydro-7-methoxy-3-(2,3,4-trimethoxyphenyl)-2H-1-benzopyran and N-[2-(3,4-dimethoxyphenyl)ethyl]-3-methoxy-benzamide. However, these substance add up to less than 1% in D. odorifera, making these two species differentiable. In a word, volatile components resemble but fat-soluble ingredients differed distinctly, which was the basis for identification of D. odorifera and D.tonkinensi with GC-MS.

参考文献/References:

[1] 国家质量技术监督局. GB/T 18107—2000红木[S].北京:中国标准出版社,2000.
[2] 李桂兰,徐蜂,罗建举,等.海南香枝木与越南香枝木木材构造特征比较解剖研究[J].广西农业生物科学,2008,27(2):154-157. Li G L, Xu F, Luo J J, et al. Comparative anatomical research on the wood structure between Dalbergia odorifera and Dalbergia tonkinensi[J]. Journal of Guangxi agricultural and biological science,2008,27(2):154-157.
[3] 罗真付,张雪峰,潘彪,等. 越南香枝木解剖构造及物理力学性能研究[J]. 安徽农业大学学报,2012, 39(4):493-496. Luo Z F, Zhang X F, Pan B, et al. Anatomy structure and physical and mechanical properties of Dalbergia tonkinensis[J]. Journal of Anhui Agricultural University,2012,39(4):493-496.
[4] 潘彪, 翟胜丞, 樊昌生.李洲坳东周古墓棺木用材树种鉴定及材性分析[J]. 南京林业大学学报(自然科学版),2013,37(3):87-91. Pan B, Zhai S C, Fan C S. Wood identification and properties analysis of the coffin timbers taken from Lizhouao ancient tomb in Jiang'an County of Jiangxi[J]. Journal of Nanjing Forestry University(natural sciences edition), 2013,37(3):87-91.
[5] 潘彪,翟胜丞,黄建秋,等.连云港藤花落史前古城遗址出土木材的树种鉴定[J]. 南京林业大学学报(自然科学版),2010,34(5):75-78. Pan B, Zhai S C, Huang J Q, et al. Identification of unearthed woods from the Tenghualuo ruins in Lianyungang[J]. Journal of Nanjing Forestry University(natural sciences edition),2010,34(5):75-78.
[6] 潘彪,翟胜丞,祁海宁. 南京大报恩寺遗址出土阿育王塔所用木材的树种鉴定[J]. 南京林业大学学报(自然科学版),2009,33(3):83-86. Pan B, Zhai S C, Qi H N.Wood identification of the wooden parts from the gilded-pagoda excavated at Dabaoen Temple site in Nanjing[J]. Journal of Nanjing Forestry University(natural sciences edition),2009,33(3):83-86.
[7] 任洪娥,高洁,马岩. 我国木材材种识别技术的新进展[J].木材加工机械, 2007,18(4):37-41. Ren H E, Gao J,Ma Y. The newly evolvement of wood recognition technology in China[J]. Wood processing machinery,2007,18(4):37-41.
[8] 唐丽丽,刘丹.几种木材树种的识别[J]. 林业勘查设计,2006(3):55-58. Tang L L, Liu D. Several kind of lumbers tree seeds recognition[J].Forest investigation design, 2006(3):55-58.
[9] 王克奇,杨少春,戴天虹,等. 基于均匀颜色空间的木材分类研究[J]. 计算机工程与设计,2008,29(7):1780-1784. Wang K Q,Yang S C,Dai T H, et al. Research on wood classification using uniform color space[J]. Computer engineering and design,2008,29(7):1780-1784.
[10] Perez-Coello M S, Sanz J, Cabezudo M D. Determination of volatile compounds in hydroalcoholic extracts of French and American oak wood[J]. American joumal of enology and viticulture, 1999, 50(2); 162-165.
[11] Balaban M. Identification of the main phenolic compounds in wood of Ceratonia siliqua by GC-MS[J]. Phytochemical analysis, 2004, 15(6):385-388.
[12] Arias M E, Polvillo O, Rodríguez J, et al. Thermal transformations of pine wood components under pyrolysis/gas chromatography/mass spectrometry conditions[J]. Journal of analytical and applied pyrolysis, 2006, 77(1): 63-67.
[13] 李艳艳,孙多永,朱仲良,等.基于气相色谱—主成分分析的红木分类识别方法研究[J].计算机与应用化学,2010,27(2):237-240. Li Y Y, Sun D Y, Zhu Z L, et al. Study on the classification and recognition of mahogany based on gas chromatograph-principle component analysis[J].Computers and applied chemistry,2010,27(2):237-240.
[14] Alexa E, Poiana M, Peev C, et al. Detection of vegetable oils adulteration on the basis of fatty acids composition delermined by RP-HPLC[J]. Revista de chimie, 2006,57(3):285-289.
[15] Marikkar J M N, Ghazali H M, Man Y B C, et al. Distinguishing lard from other animal fats in admixtures of some vegetable oils using liquid chromatographic data coupled with multivariate data analysis[J]. Food chemistry, 2005, 91(1): 5-14.
[16] Azadmard-Damirchi S, Dutta P C. Rapid separation of methylsterols from vegetable oils by solid-phase extraction[J]. Lipid technology, 2006, 18(10): 231.
[17] 杨柳,吴翠蓉,朱杰丽,等. 顶空-气质联用法鉴别油茶籽油真伪[J].中国粮油学报,2012,27(11):105-109,113. ang L, Wu C R, Zhu J L, et al. Identification of authentic or fake of Camellia oil by headspace gas chromatography-mass Spectrometry[J]. Journal of the Chinese cereals and oils association, 2012,27(11):105-109,113.
[18] 杨柳,方崇荣,于海霞,等.顶空/气质联用鉴别交趾黄檀与微凹黄檀[J].中南林业科技大学学报,2013,33(12):34-39. Yang L, Fang C R, Yu H X, et al. Identification of Dalbergia cochinchinenses and Dalbergia retusa by headspace gas chromatography-mass spectrometry[J]. Journal of Central South University of forestry and technology, 2013,33(12):34-39.
[19] 杨柳,方崇荣,王增,等.檀香紫檀中可挥发性成分对其材种鉴定的初步研究[J]. 浙江林业科技,2013,33(1):40-44. Yang L, Fang C R, Wang Z, et al. Identification of Pterocarpus santalinus Wood by determination of volatile compounds[J]. Journal of Zhejiang forestry science and technology, 2013,33(1):40-44.

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备注/Memo

备注/Memo:
收稿日期:2014-06-04 修回日期:2014-10-08
基金项目:浙江省重点科技创新团队“浙江省木材加工产业科技创新团队”资助项目(2012R10023-04)。
第一作者:杨柳(yangliuxs@163.com)。
引文格式:杨柳,方崇荣,张建,等. 气质联用鉴别降香黄檀与越南香枝的研究[J]. 南京林业大学学报(自然科学版),2016,40(1):97-103.
更新日期/Last Update: 2016-02-25