[1]冯源恒,杨章旗*,李火根*,等.不同时期广西马尾松优良种源的遗传多样性变化趋势[J].南京林业大学学报(自然科学版),2016,40(05):041-46.[doi:10.3969/j.issn.1000-2006.2016.05.007]
 FENG Yuanheng,YANG Zhangqi*,LI Huogen*,et al.A study on changes of genetic diversity for nearly 50 years in superior provenances of Pinus massoniana in Guangxi[J].Journal of Nanjing Forestry University(Natural Science Edition),2016,40(05):041-46.[doi:10.3969/j.issn.1000-2006.2016.05.007]
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不同时期广西马尾松优良种源的遗传多样性变化趋势
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
40
期数:
2016年05期
页码:
041-46
栏目:
研究论文
出版日期:
2016-09-30

文章信息/Info

Title:
A study on changes of genetic diversity for nearly 50 years in superior provenances of Pinus massoniana in Guangxi
文章编号:
1000-2006(2016)05-0041-06
作者:
冯源恒12杨章旗1*李火根2*罗群凤1吴东山1黄永利3
1.广西壮族自治区林业科学研究院,广西 南宁 530002;
2.南京林业大学,林木遗传与生物技术省部共建教育部 重点实验室,江苏 南京 210037;
3.南宁市林业科学研究所,广西 南宁 530107
Author(s):
FENG Yuanheng12 YANG Zhangqi1* LI Huogen2*LUO Qunfeng1 WU Dongshan1 HUANG Yongli3
1.Guangxi Institute of Forestry Science,Nanning 530002,China;
2. Key Lab of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, China;
3. Nanning Forestry Division, Nanning 530107, China
关键词:
马尾松 种源 遗传多样性 SSR
Keywords:
Pinus massoniana provenance genetic diversity SSR
分类号:
S718.46; Q347
DOI:
10.3969/j.issn.1000-2006.2016.05.007
文献标志码:
A
摘要:
马尾松是我国南方重要的乡土树种,近30年来,受人为干扰和破坏十分严重。为了解目前广西马尾松天然遗传资源现状,评价当前种质资源收集对遗传多样性保护的有效程度,采用SSR标记对3个时期收集的广西马尾松3个优良种源进行遗传多样性研究。结果表明:现阶段,马尾松桐棉种源群体的Shannon 多样性指数(I)为 0.64,观测杂合度(Ho)为 0.35,期望杂合度(He)为 0.36; 古蓬种源群体Shannon 多样性指数(I)为 0.53,Ho为 0.36, He为 0.32。说明广西马尾松遗传多样性仍处于较高水平。将3个种源的遗传多样性进行对比,发现桐棉种源的遗传多样性最高,古蓬种源次之,云开大山种源相对较低。群体间遗传分化系数(GST)为0.04,Wright固定指数(FST)平均值为0.026,基因流(Nm)大小为9.56,说明3个种源间不存在明显的遗传分化,群体间基因交流顺畅。将不同时期的遗传多样性进行对比分析可知:桐棉种源在18 a内 Shannon 多样性指数(I)下降了5%、期望杂合度(He)下降了10%; 古蓬种源Shannon 多样性指数下降了13%、期望杂合度(He)下降了14%。说明随着时间的推移,马尾松天然资源受到人为干扰愈来愈强烈,遗传多样性正逐步丧失,急需开展有针对性的保护。
Abstract:
In view of the situation of constant human disturbance to the natural resources of Guangxi Pinus massoniana(masson pine), the dynamic changes of the genetic diversity in provenances of masson pine were studied to provide important reference for the protection of the natural germplasm resources of masson pine. Sixteen SSR molecular markers were used to analyze the genetic diversity of eight populations in three provenances from Guangxi Tongmian, Gupeng and Yunkai Mountain which were collected in 1950s, 1980s and 2012. At the present stage, the mean number of Shannon's information index(I), observed heterozygosity(Ho)and expected heterozygosity(He)in Tongmian provenance were 0.64, 0.35 and 0.36 respectively, while Gupeng provenance were 0.53, 0.36 and 0.32 respectively. Compared the genetic diversity level of the 3 provenances, Tongmian provenance possessed the highest genetic diversity, Gupeng followed, Yunkai mountain was relatively low. Coefficient of genetic differentiation among populations(GST)was 0.04, average FST was 0.026, gene flow(Nm)was 9.56. The results showed that no significant genetic differentiation was available among three provenances, and gene flow among them was smooth. Through the comparing and analyzing of genetic diversity of different periods, the Shannon's information index(I)and expected heterozygosity(He)of Tongmian provenance decreased by 5% and 10% within 18 years, and Gupeng provenance dropped 13% and 14%. Which indicated that over the time masson pine natural resources were affected by human interference more and more, genetic diversity is gradually losing, we urgently need to carry out targeted protection.

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备注/Memo

备注/Memo:
收稿日期:2015-11-12 修回日期:2016-05-08
基金项目:“十三五”国家科技支撑计划(2015BAD09B0102-2); 广西自然科学基金项目(2014GXNSFBA118078); 广西优良用材林资源培育重点实验室自主研究课题(12B0102)
第一作者:冯源恒(nanyuan05@163.com),高级工程师,博士。*通信作者:杨章旗(yangzhangqi@163.com),教授级高级工程师,博士; 提供研究材料及早期基础研究数据,对研究与分析思路进行指导。李火根(hgli@njfu.edu.cn),教授,博士; 对试验设计及数据分析进行指导,并提供实验平台。
引文格式:冯源恒,杨章旗,李火根,等. 不同时期广西马尾松优良种源的遗传多样性变化趋势[J]. 南京林业大学学报(自然科学版),2016,40(5):41-46.
更新日期/Last Update: 2016-10-30