[1]杨 鑫,张高洁,姚继周,等.水杉人工林细根解剖结构和菌根侵染研究[J].南京林业大学学报(自然科学版),2016,40(06):097-102.[doi:10.3969/j.issn.1000-2006.2016.06.015]
 YANG Xin,ZHANG Gaojie,YAO Jizhou,et al.Study on fine root anatomical structure and mycorrhizal colonization in Metasequoia glyptostroboides plantation[J].Journal of Nanjing Forestry University(Natural Science Edition),2016,40(06):097-102.[doi:10.3969/j.issn.1000-2006.2016.06.015]





Study on fine root anatomical structure and mycorrhizal colonization in Metasequoia glyptostroboides plantation
杨 鑫张高洁姚继周于水强*方月丰
南京林业大学,南方现代林业协同创新中心,江苏 南京 210037
YANG Xin ZHANG Gaojie YAO Jizhou YU Shuiqiang* FANG Yuefeng
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037,China
水杉 细根 根序 解剖结构 菌根侵染
Metasequoia glyptostroboides fine root root order anatomical structure mycorrhizal colonization
S718.3; Q945.79
Tree root system is one of the most complex parts of the underground ecosystem. The accuracy and comparability in root ecological function studies is limited by highly heterogeneous morphology and structure. The reasonability of definition on fine roots of Metasequoia glyptostroboides was discussed which could improve fine root studies in the future. We determined fine root anatomical structures and root mycorrhizal colonization with different orders in M. glyptostroboides plantation and explored the relationship between fine root morphology and anatomy. We found: firstly, it was more reasonable to classify the roots by root order, and 1,2 level roots were defined as fine roots; secondly,1,2 level roots were those ones with intact epidermis, thicker and multi-layered cortex, and 3, 4 level roots were skinless roots or only a small amount of residual epidermal cells, with root orders increased, roots cortex layers and epidermis thickness became significantly lower; thirdly, with the root order increasing, the degree of cork periderm was significantly increased, vascular tissue development was gradually improved, the diameter of the vascular tissue and the ratio of vascular tissue to root diameter(V/R)was increasing with root order increase. There were significant correlation(except root level)between fine root diameter and vascular tissue diameter, but there was no correlation between fine root diameter and the cortical parenchyma cells diameter or cortical layers; fourthly,the mycorrhizal colonization in 1-3 order roots could be observed, and with root order increase, the mycorrhizal colonization rate was significantly decreased. There were obvious differences in fungal infestation area of single individual root with different order. In the future study, we should combine fine root morphology investigation and anatomy characteristics to investigate the correspondence between the root order and root diameter, and then determine the division of the range of fine roots.


[1] Jackson R B, Mooney H A,Schulze E D. A global budget for fine root biomass, surface area, and nutrient contents[J]. Proceedings of the National Academy of Sciences,1997,94(14): 7362-7366.
[2] Gill R A,Jackson R B. Global patterns of root turnover for terrestrial ecosystems[J]. New Phytologist, 2000, 147(1): 13-31. Doi:10.1007/s12665-013-2482-0.
[3] McCormack M L, Guo D. Impacts of environmental factors on fine root lifespan[J]. Frontiers in Plant Science, 2014,5(205):1-11. Doi: 10.3389/fpls.2014.00205.
[4] Eissenstat D M, Wells C E,Yanai R D,et al.Building fine roots in a changing environment: implications for root longevity[J]. New Phytologist,2000,147(1):33-42. Doi: 10.1046/j.1469-8137.2000.00686.x.
[5] Eissenstat D M,Yanai R D. The ecology of root lifespan[J]. Advances in Ecological Research, 1997, 27: 1-30. Doi: 10.1016/S0065-2504(08)60005-7.
[6] Brundrett M,Bougher N,Dell B, et al. Working with mycorrhizas in forestry and agriculture[J]. Australian Centre for International Agricultural Research, 1996,32: 374.
[7] McCormack M L,Dickie I A,David M E,et al. Redefining fine roots improves understanding of belowground contributions to terrestrial biosphere processes[J]. New Phytologist, 2015,207(3): 505-518. Doi:10.1111/nph.13363.
[8] McCormack M L, Zeng H,Eissenstat D M, et al. Variation of first-order root traits across climatic gradients and evolutionary trends in geological time[J]. Global Ecology and Biogeography, 2013, 22(7): 846-856. Doi: 10.1111/geb.12048.
[9] Eissenstat D M,Kucharski J M,Marcin Z. Linking root traits to nutrient foraging in arbuscular mycorrhizal trees in a temperate forest[J]. New Phytologist, 2015, 208(1): 114-124. Doi: 10.1111/nph.13451.
[10] Liu B, Li H, Zhu B,et al. Complementarity in nutrient foraging strategies of absorptive fine roots and arbuscular mycorrhizal fungi across 14 coexisting subtropical tree species[J]. New Phytologist, 2015, 208(1):124-136.Doi: 10.1111/nph.13434.
[11] Guo D L, Li H,Robert J M, et al. Fine root heterogeneity by branch order: exploring the discrepancy in root turnover estimates between minirhizotron and carbon isotopic methods[J]. New Phytologist, 2008, 177(2): 443-456.Doi: 10.1111/j.1469-8137.2007.02242.x.
[12] 王向荣,王政权,韩有志,等.水曲柳和落叶松不同根序之间细根直径的变异研究[J]. 植物生态学报, 2005, 29(6):871-877.Doi: 10.1007/s11461-007-0005-4. Wang X R,Wang Z Q,Han Y Z,et al.Researches on root diameter variation between the Fraxinus mandshurica and Larix gmelinii different root sequences [J]. Journal of Plant Ecology, 2005, 29(6):871-877.
[13] Vogt K A,Persson H.Measuring growth and development of roots[C]// Lassoie J P, Hinckley T M. Techniques and approaches in forest tree ecophysiolog. Boca Raton Chemical Rubber Company, 1991:477-502.
[14] Pregitzer K S,Forest D J,Andrew J B,et al. Fine root architecture of nine North American trees[J]. Ecological Monographs,2002,72(2):293-309.Doi:10.1890/0012-9615(2002)072[0293:FRAONN]2.0.CO; 2.
[15] Vogt K A,Vogt D J,Bloomfield J. Analysis of some direct and indirect methods for estimating root biomass and production of forests at an ecosystem level[J].Plant and Soil, 1998, 200(1):687-720. Doi: 10.1007.1978-94-011-5270-9_61.
[16] 卫星,刘颖,陈海波.黄波罗不同根序的解剖结构及其功能异质性[J].植物生态学报,2008,32(6):1238 -1247. Doi: 10.3773/j.issn.1005-264x.2008.06.004. Wei X,Liu Y,Chen H B.Anatomical and functional heterogeneity among different root orders of Phellodendeon amurense [J]. Journal of Plant Ecology, 2008,32(6):1238 -1247.
[17] Hishi T,Takeda H. Dynamics of heterorhizic root systems: Protoxylem groups within the fine-root system of Chamaecyparis obtusa [J]. New Phytologist, 2005, 167(2): 509-521.Doi: 10.1111/j.1469-8137.2005.01418.x.
[18] Hishi H. Heterogeneity of individual roots within the fine root architecture: causal links between physiological and ecosystem functions[J]. Journal of Forest Research, 2007, 12(2): 126- 133.Doi: 10.1007/s10310-006-0260-5.
[19] Li A,Guo D L,Wang Z Q,et al. Nitrogen and phosphorus allocation in leaves, twigs,and fine roots across 49 temperate, subtropical and tropical tree species: a hierarchical pattern[J]. Functional Ecology, 2010, 24(1): 224-232.Doi: 10.1111/j.1365-2435.2009.01603.x.
[20] Wells C E, Eissenstat D M. Marked differences in survivorship among apple roots of different diameters[J]. Ecology, 2001, 82(3): 882- 892. Doi: 10.2307/2680206.
[21] 于水强, 王政权, 史建伟, 等. 氮肥对水曲柳和落叶松细根寿命的影响[J]. 应用生态学报, 2009, 20(10): 2332-2338. Doi:10.13287/j.1001 -9332.2009.0322. Yu S Q,Wang Z Q,Shi J W,et al.Effect of nitrogen fertilizer on leaf and fine root lifespan of Fraxinus mandshurica and Larix gmelinii[J]. Journal of Applied Ecology, 2009, 20(10): 2332-2338.
[22] Jia S X, Wang Z Q,Li X P, et al. Effect of nitrogen fertilizer, root branch order and temperature on respiration and tissue N concentration of fine roots in Larix gmelinii and Fraxinus mandshurica[J]. Tree Physiology, 2011, 31(7):718-726. Doi: 10.1093/treephys/tpr057.
[23] McCormack M L,Thomas S A,Erica A H, et al. Variability in root production phenology, and turnover rate among 12 temperate tree species[J]. Ecology, 2014, 95(8): 2224-2235.Doi:10.1890/13-1942.1.
[24] 史建伟,秦晴,陈建文.柠条人工林细根不同分枝根序寿命估计[J].生态学报,2015,35(12):4045-4052.Doi:10.5846/stxb201405231064. Shi J W,Qin Q,Chen J W.Caragana plantation in different branches of fine root root sequence lifetime estimation [J]. Journal of Applied Ecology,2015,35(12):4045-4052.
[25] Goebel M,Hobbie S E,Bula J B,et,al. Decomposition of the finest root branching orders:linking belowground dynamics to fine-root function and structure[J]. Ecological Monographs, 2011, 81(1): 89-102.Doi: 10.1890/09-2390.1.
[26] 谭小明, 郭顺星. 红豆杉根的显微结构及其内生真菌分布[J]. 中国医学科学院学报, 2006,28(3):372-374.Doi: 1000-503X(2006)03-0372-03. Tan X M,Guo S X.Root microstructure and distribution of the endophytic fungi in Taxus chinensis var. mairei[J].Chinese Academy of Medical Sciences,2006,28(3):372-374.


 WANG Liang-gui,ZHU Qiang-gen,ZHANG Huan-chao*,et al.Annual production and turnover rate of fine roots in poplar plantations in north of Jiangsu[J].Journal of Nanjing Forestry University(Natural Science Edition),2008,32(06):076.[doi:10.3969/j.jssn.1000-2006.2008.05.015]
 Wang Peiqing,Yu Jun & Li Mindong.STUDIES ON STRUCTURES OF THE GALACTOGLUCOMANNANS FROM METASEQUOIA GLYPTOSTROBOIDES[J].Journal of Nanjing Forestry University(Natural Science Edition),1986,10(06):066.[doi:10.3969/j.jssn.1000-2006.1986.02.008]
 Zhou Lin Ma Yixiou Zhang Hongzhong Jiang Jiangang(Forestry and Pasture Bureau of Jianhu County of Jiangsu Province Jianhu 700)Zhu Guosheng.IMPACTS OF THINNING ON THE VOLUME INCREMENT AND REVENUES REVENUES OF METASEQUOIA GLYPTOSTROBOIDES PLANTATIONS[J].Journal of Nanjing Forestry University(Natural Science Edition),1997,21(06):093.[doi:10.3969/j.jssn.1000-2006.1997.02.021]
 Author Wei ChunChen JianqunZhang PengfeiZhang XianChen Gang (Department of Biological Science and Technology Nanjing UniversityNanjing009) Zhang Jilin.The Biossay of Allelopathy of Metasequoia glyptostroboides in Agroforesty System[J].Journal of Nanjing Forestry University(Natural Science Edition),1999,23(06):085.[doi:10.3969/j.jssn.1000-2006.1999.04.019]
[6]卢东升,卢 帅,潘中超,等.水杉内生真菌生物多样性与生态分布[J].南京林业大学学报(自然科学版),2013,37(06):033.[doi:10.3969/j.issn.1000-2006.2013.06.007]
 LU Dongsheng,LU Shuai,PAN Zhongchao,et al.Biological diversity and ecological distribution of endophytic fungi in Metasequoia glyptostroboides[J].Journal of Nanjing Forestry University(Natural Science Edition),2013,37(06):033.[doi:10.3969/j.issn.1000-2006.2013.06.007]
 XU Yu,XU Kai,WANG Wenjuan,et al.The response of carbohydrates compositions in fine root of poplar at different ages to nitrogen depositions[J].Journal of Nanjing Forestry University(Natural Science Edition),2014,38(06):013.[doi:10.3969/j.issn.1000-2006.2014.03.003]
 YUAN Zaixiang,JIN Xuemei,ZHAI Kaiyan,et al.Effects of common urban tree species on vertical distribution of soil carbon & nitrogen and organic carbon storage[J].Journal of Nanjing Forestry University(Natural Science Edition),2018,42(06):153.[doi:10.3969/j.issn.1000-2006.201706016]


基金项目:国家自然科学基金项目(31270489); 国家重点基础研究发展计划(2012CB416904); 江苏高校优势学科建设工程资助项目(PAPD)
引文格式:杨鑫,张高洁,姚继周,等. 水杉人工林细根解剖结构和菌根侵染研究[J]. 南京林业大学学报(自然科学版),2016,40(6):97-102.
更新日期/Last Update: 2016-11-20