[1]张往祥,范俊俊,杨 萍,等.‘紫王子’海棠半同胞家系苗期表型特征分析[J].南京林业大学学报(自然科学版),2017,41(06):033-40.[doi:10.3969/j.issn.1000-2006.201708012]
 ZHANG Wangxiang,FAN Junjun,YANG Ping,et al.Analysis of phenotypic characteristics of the Malus ‘Purple Prince’ half-sib progenies at the seedling stage[J].Journal of Nanjing Forestry University(Natural Science Edition),2017,41(06):033-40.[doi:10.3969/j.issn.1000-2006.201708012]





Analysis of phenotypic characteristics of the Malus ‘Purple Prince’ half-sib progenies at the seedling stage
张往祥12范俊俊1杨 萍3周 婷1浦 静1曹福亮1
1.南方现代林业协同创新中心,南京林业大学林学院,江苏 南京 210037; 2.扬州小苹果园艺有限公司,江苏 扬州 225200; 3.罗甸县林业局,贵州 黔南 555128
ZHANG Wangxiang12 FAN Junjun1 YANG Ping3 ZHOU Ting1PU Jing1 CAO Fuliang
1.Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China; 2. Yangzhou Crabapple Horticulture Limited Company, Yangzhou 225200, China; 3. Luodian Forestry Bureau, Qiannan 555128, China
紫王子’海棠 半同胞家系 表型多样性 超亲性 优株评价
Keywords:Malus ‘Purple Prince’ half-sib progenies phenotypic diversity transgressive characteristics comprehensive evaluation
【目的】基于海棠种质基因库优良品种资源丰富的优势,开展了以‘紫王子’海棠(Malus ‘Purple Prince’)为母本的自由授粉子代试验研究,探索其半同胞家系表型特征及育种成效。【方法】运用Shannon-Wiener(HR)及Pielou(ER)等指数进行14个性状的表型多样性比较,采用频率分布函数法进行亲子代间性状变异趋势及超亲性分析,通过层次分析法(AHP)进行表型性状综合评价。【结果】在表型多样性方面,‘紫王子’海棠半同胞家系14个性状的HR平均值比疑似父本群体提高了13%,其中地径生长量、分枝数、完全功能叶颜色的HR和ER超过疑似父本群体23.8%~53.2%。在遗传改良方面,与疑似父本群体相比,半同胞家系中综合性状得分高的优株比例下降了37.0%,紫红叶优株和速生优株的比例分别下降了14.9%和37.5%,但枫叶型、分枝多、通直度高、耐热性强的优株比例分别增加了12.6%、48.3%、28.7%和18.5%; 与母本相比,7.8%的优株综合性状得分超过母本; 此外,有些单株综合得分略低于母本,但部分单一性状表现比较突出,如F113和F60优株的新叶为鲜见的亮红色,F35和F108优株的叶片为枫叶型,观赏效果佳。【结论】尽管自由授粉降低了优株比例,但有效扩大了子代群体的遗传基础,对部分性状的遗传改良具有明显的效果。
【Objective】In this project, experiment was conducted on the free pollination progeny of Malus ‘Purple Prince’ based on the advantages of the abundant resources in a crabapple germplasm bank. The goal of this study was to explore the phenotypic characteristics and breeding effectiveness of half-sib progenies.【Method】The phenotypic diversity of 14 traits was compared using the value of Shannon-Wiener(HR)and Pielou indexes(ER). The trends in variation and transgressive characteristics of traits between parents and progeny were analyzed using a frequency distribution function. Subsequently, the comprehensive evaluation of phenotypic traits was conducted by the analytic hierarchy process(AHP). 【Result】In terms of phenotypic diversity, the average HR of the 14 traits of the half-sib progenies increased by 13% compared to that of the possible paternal parent group. Among them, the HR and ER of the ground diameter growth, branch number, heat resistance and mature leaf color increased by 23.8%-53.2%. Regarding genetic improvement, the proportion of excellent seedlings with high comprehensive scores in the half-sib progenies decreased by 37.0% compared with the possible paternal parent group. The proportion of seedlings with purple-red colored leaves or a fast-growing character decreased by 14.9% and 37.5%, respectively. However, the proportion of seedlings with maple leaves, many branches, high seedling straightness, or high heat resistance increased by 12.6%, 48.3%, 28.7% and 18.5%, respectively. Compared with the maternal parent clones, the proportion of seedlings with high comprehensive scores increased by 7.8%(12 seedlings). In addition, although the comprehensive score of some individual was slightly lower than that of the maternal parent clones, some single traits performed notably well. For example, the young leaves of F113 and F60 excellent seedlings were rarely bright red, the leaf type of F35 and F108 excellent seedlings as maple.【Conclusions】Although free pollination reduced the proportion of excellent seedlings with high comprehensive scores, it effectively expanded the genetic basis of half-sib progenies. Furthermore, it had obvious effect on the genetic improvement of some single traits.


[1] 李育农. 苹果属植物种质资源研究[M]. 北京:中国农业出版社, 2001: 181-183; 315-335.
[2] REHDER A. Manual of cultivated trees and shrubs[M]. New York: Macmillam Co, 1940: 389-399.
[3] FIALA J L. Flowering crabapples[M]. Portland: Timber Press, 1994:106-273.
[4] HOFER M, MOHAMED M A, ALI S E, et al. Phenotypic evaluation and characterization of a collection of Malus species[J]. Genetic Resources & Crop Evolution, 2014, 61(5):943-964. DOI: 10.1007/s10722-014-0088-3.
[5] 刘志强, 汤庚国. 南京地区垂丝海棠品种分类研究[J]. 南京林业大学学报(自然科学版), 2004, 28(1):101-106. DOI: 10. 3969/j. jssn. 1000-2006. 2004. 01. 026.LIU Z Q, TANG G G. A study on cultivar classification system of Malus halliana Koehne[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2004, 28(1): 101-106.
[6] CHANTA-MELLIZAO A, CAMPBELL S A, BONILLA M A, et al. Effects of natural and artificial pollination on fruit and offspring quality[J]. Basic & Applied Ecology, 2012, 13(6): 524-532. DOI: 10. 1016/j. baae. 2012. 08. 013.
[7] HONSHO C, YONEMORI K, SOMSRI S, et al. Marked improvement of fruit set in Thai durian by artificial cross-pollination[J]. Scientia Horticulturae, 2004, 101(4): 399-406. DOI: 10. 1016/j. scienta. 2003. 11. 019.
[8] STONE J L, THOMSON J D, DENT-ACOSTA S J. Assessment of pollen viability in hand-pollination experiments: a review[J]. American Journal of Botany, 1995, 82(9): 1186-1197. DOI: 10. 2307/2446073.
[9] MUNZUROGLU O, OBEK E H. Effects of simulated acid rain on the pollen germination and pollen tube growth of apple(Malus sylvestris Miller cv. Golden)[J]. Acta Biologica Hungarica, 2003, 54(1): 95-103. DOI: 10. 1002/anie. 200500382.
[10] 刘会超, 贾文庆. 离体萌发法测定垂丝海棠花粉生活力的研究[J]. 山西农业科学, 2007, 35(5):39-41. DOI: 10. 3969/j.issn. 1002-2481. 2007. 05. 011.LIU H C, JIA W Q. Determination on pollen viability of Malus halliana[J]. Journal of Shanxi Agricultural Sciences, 2007, 35(5): 39-41.
[11] 张素华, 吕晓燕, 王奇. 西府海棠花粉生活力最佳培养基的研究[J]. 河南林业科技, 2013, 33(3): 5-7.ZHANG S H, LV X Y, WANG Q. Study on the optimum media of pollen vitality of Malus micromalus Makino[J]. Journal of Henan Forestry Science and Technology, 2013, 33(3): 5-7.
[12] 乌吉玛, 德英, 斯琴巴特尔. 自交、异交及开放授粉对不同熟性麦宾草结实率的影响[J]. 内蒙古农业科技, 2013(3): 28-29. DOI: 10.3969/j. jssn. 1007-0907. 2013. 03. 014.WU J M, DE Y, Sechenbater. Effect of inbred, out breeding and open pollination on setting percentage of different maturity Elymus tangutorum[J]. Inner Mongolia Agricultural Science and Technology, 2013(3): 28-29.
[13] GVARYAHU G, ROBINZON B, MELTZER A, et al. Artificial insemination and natural mating in the crossbreeding of the Muscovy drake and the Pekinduck[J]. Poultry Science, 1984, 63(2): 386. DOI: 10. 3382/ps. 0630386.
[14] COHEN L, POSCH T E. Positive time-frequency distribution functions[J]. IEEE Transactions on Acoustics Speech & Signal Processing, 1985, 33(1): 31-38. DOI: 10.1109/TASSP. 1985. 1164512.
[15] VAIDYA O S, KUMAR S. Analytic hierarchy process: an overview of applications[J]. European Journal of operational research, 2006, 169(1): 1-29. DOI: 10.1016/j.ejor. 2004. 04. 028.
[16] Van NOCKER S, BERRY G, NAJDOWSKI J, et al. Genetic diversity of red-fleshed apples(Malus)[J]. Euphytica, 2012, 185(2): 281-293. DOI: 10.1007/s10681-011-0579-7.
[17] ELLIS B, DALY D, HICKEY L J, et al. Manual of leaf architecture[M]. New York: Comstock Publishing Associates, Cornell University Press, 2009.
[18] 郭金玉, 张忠彬, 孙庆云. 层次分析法的研究与应用[J]. 中国安全科学学报, 2008, 18(5):148-153.DOI:10.3969/j.issn.1003-3033.2008.05.025.GUO J Y,ZHANG Z B, SUN Q Y. Study and applications of analytic hierarchy process[J]. China Safety Science Journal, 2008, 18(5): 148-153.
[19] 俞德浚. 中国植物志:第36卷[M]. 北京:科学出版社, 1974: 372-402.
[20] 李艳艳. 玫瑰花产量性状、观赏特性的遗传变异及选育策略研究[D]. 泰安:山东农业大学, 2008. LI Y Y. Genetic variation and ornamental strategy of yield characters, ornamental characters in roses[D]. Tai’an: Shangdong Agricultural University, 2008.
[21] JEFFERSON R M. History, progeny, and locations of crabapples of documented authentic origin[M]. Washington D C: U.S. Department of Agricultures, 1970.
[22] 陈忠文, 文西强, 王宏飞. 不同种植方式和距离对豆薯异交结实率的影响[J]. 中国种业, 2014(5): 49-50.CHEN Z W, WEN X Q, WANG H F. Effects of different planting patterns and distance on the seed setting rate of outcrossing Yambean[J]. China Seed Industry, 2014(5): 49-50.


 TONG Fang-ping,FANG Wei,MA Lij-yi,et al.Study on Response to Photosynthetic Characters of Slash Pine’s Half-sib Under Water Stress[J].Journal of Nanjing Forestry University(Natural Science Edition),2007,31(06):032.[doi:10.3969/j.jssn.1000-2006.2007.02.008]


基金项目:江苏省科技厅重点研发计划(BE2016388); 江苏省农业科技自主创新资金项目(CX(16)1005-4-1) 第一作者:张往祥(malus2011@163.com),副教授,博士。*通信作者:E-mail: ××。
更新日期/Last Update: 1900-01-01