[1]王亚召,嵇保中*,刘曙雯*,等.菌圃材料对黑翅土白蚁采食工蚁踪迹活性的影响[J].南京林业大学学报(自然科学版),2017,41(06):073-78.[doi:10.3969/j.issn.1000-2006.201604016]
 Trail-following activity of Odontotermes formosanus foragers for fungus-garden materials.Trail-following activity of Odontotermes formosanus foragers for fungus-garden materials[J].Journal of Nanjing Forestry University(Natural Science Edition),2017,41(06):073-78.[doi:10.3969/j.issn.1000-2006.201604016]
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菌圃材料对黑翅土白蚁采食工蚁踪迹活性的影响
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
41
期数:
2017年06期
页码:
073-78
栏目:
研究论文
出版日期:
2017-11-30

文章信息/Info

Title:
Trail-following activity of Odontotermes formosanus foragers for fungus-garden materials
文章编号:
1000-2006(2017)06-0073-06
作者:
王亚召1嵇保中1*刘曙雯2*丁 芳1徐立军1
1. 南方现代林业协同创新中心,南京林业大学林学院,江苏 南京 210037; 2. 南京中山陵园管理局,江苏 南京 210014
Author(s):
Trail-following activity of Odontotermes formosanus foragers for fungus-garden materials
1. Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037,China; 2. The Administration Bureau of Dr. Sun Yat-sen's Mausoleum, Nanjing 210014,China
关键词:
黑翅土白蚁 主巢菌圃 共生真菌 采食工蚁 踪迹活性
Keywords:
Keywords:Odontotermes formosanus fungus garden of insect chamber symbiotic fungus foragers trail-following activity
分类号:
S764
DOI:
10.3969/j.issn.1000-2006.201604016
文献标志码:
A
摘要:
【目的】研究菌圃、杂交鹅掌楸树干外表皮、密粘褶菌松木粉培养物共3种提取液对黑翅土白蚁采食工蚁的踪迹活性,揭示培菌白蚁在食物采集、加工、分配过程中信息联系机制,以便开发出针对培菌白蚁的高效食性饵剂。【方法】利用划线法测定采食工蚁对上、中、下层主巢菌圃与副巢菌圃的踪迹反应率与选择系数,选出最佳提取试剂; 比较菌圃、杂交鹅掌楸外表皮、密粘褶菌松木粉培养物提取液对采食工蚁的踪迹活性,测定采食工蚁对菌圃提取液踪迹活性的浓度效应。【结果】正己烷提取液的踪迹活性最高,乙醇、丙酮和乙酸乙酯提取液之间踪迹活性呈现不规律变化,蒸馏水提取液踪迹活性最低; 主巢下层菌圃踪迹活性物质含量高,为最佳提取材料; 经拟合,得到浓度(x)-踪迹反应率(y)方程:y = 1.695 8x3-13.346x2 + 30.137x + 33.409。【结论】菌圃材料最佳提取溶剂为正已烷,黑翅土白蚁共生菌圃对采食工蚁具有明显的踪迹活性,踪迹活性强度显著高于林间采集材料(杂交鹅掌楸树干外表皮)的影响,与密粘褶菌松木粉培养物相近。采食工蚁在主巢下层菌圃踪迹活性最高,上层菌圃次之,中层菌圃和副巢菌圃最低,但相互之间差异不显著。
Abstract:
【Objective】Odontotermes formosanus is an important fungus-growing termite. In this study, we investigated trail-following activity of O. formosanus foragers of extracts of fungus garden, outer epidermis of the trunk of Liriodendron chinense× L. tulipifera,and pine wood powder infected by Gloeophyllum trabeum(PWG). 【Method】The rate of trail-following response and selection coefficient of the different materials were evaluated using the streak plate method. The analyzed materials included the top layer of fungus garden in the main chamber(TFM), middle layer of fungus garden in the main chamber(MFM), bottom layer of fungus garden in the main chamber(BFM), fungus garden in the satellite chamber(FSC).【Result】From the results, the best chemical was determined. Furthermore, trail-following activities were compared using extracts from the fungus garden, TEL and PWG. Moreover, the effects of concentrations of the fungus garden extracts on trail-following activity were studied. O. formosanus foragers showed the highest trail-following activity for N-hexane; trail-following activities for ethyl alcohol, acetone, and ethyl acetate varied irregularly, and were the lowest for distilled water. BFM induced the highest trail-following activity among all analyzed fungus-garden materials, therefore, it was best for extraction. The linear relationship between the concentration of N-hexane solution(x)and rate of trail-following response(y)could be expressed by the equation:y = 1.695 8x3-13.346x2 + 30.137x + 33.409.【Conclusion】Regarding the extracts for trail-following activity, N-hexane was the best chemical. The fungus garden in the O. formosanus chambers induced obviously trail-following activity in the foragers. The trail-following activity for the fungus garden was fairly higher than that for the field-collected materials, and similar for PWG. BFM induced the highest trail-following activity, followed by TFM, and MFM and FSC induced the lowest activity. However, there were no significant differences among them.

参考文献/References:

[1] 张新慰, 嵇保中, 刘曙雯, 等. 白蚁消化系统解剖构造及共生物研究进展[J]. 南京林业大学学报(自然科学版), 2015, 39(1): 155-161. DOI: 10.3969/j.issn.1000-2006.2015.01.028. ZHANG X W, JI B Z, LIU S W, et al. Research progress inanatomic structures of digestive system and symbiotes in termites[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2015, 39(1): 155-161.
[2] HINZE B, CRAILSHEIM K, LEUTHOLD R H. Polyethism in food processing and social organisation in the nest of Macrotermes bellicosus(Isoptera, Termitidae)[J]. Insectes Sociaux, 2002, 49(1): 31-37. DOI: 10.1007/s00040-002-8275-1.
[3] HONGOH Y, EKPORNPRASIT L, INOUE T, et al. Intracolony variation of bacterial gut microbiota among castes and ages in the fungus-growing termite Macrotermes gilvus[J]. Molecular Ecology, 2006, 15(2): 505-516. DOI: 10.1111/j.1365-294X.2005.02795.x.
[4] BADERSCHER S, GERBER C, LEUTHOLD R H. Polyethism in food supply and processing in termite colonies of Macrotermes subhyalinus(Isoptera)[J]. Behavioral Ecology and Sociobiology, 1983, 12(2): 115-119. DOI: 10.1007/BF00343201.
[5] VEIVERS P C, MUHLEMANN R, SLAYTOR M, et al. Digestion, diet and polyethism in two fungus-growing termites:Macrotermes subhyalinus Rambur and M. michaelseni Sjostedt[J]. Journal of Insect Physiology, 1991, 37(9): 675-682. DOI: 10.1016/0022-1910(91)90044-Z.
[6] NOBRE T, ROULAND-LEFEVRE C, AANEN D K. Comparative biology of fungus cultivation in termites and ants [C]//BIGNELL D E, ROISIN Y, LO N. Biology of termites: a modern synthesis. Berlin: Springer Verlag Press, 2010, 193-210. DOI: 10.1007/978-90-481-3977-4_8.
[7] 王亚召, 嵇保中, 刘曙雯, 等. 白蚁取食行为多型及其机理[J]. 环境昆虫学报, 2016, 38(1): 181-192. DOI: 10. 3969/j. issn. 1674-0858. 2016. 01. 24. WANG Y Z, JI B Z, LIU S W, et al. Feeding polyethism and its mechanism of termites[J]. Journal of Environmental Entomology, 2016, 38(1): 181-192.
[8] 李鸿杰. 黑翅土白蚁及其微生物协同降解木质纤维素机制研究[D]. 杭州: 浙江大学, 2014. LI H J. The mechanism of lignocellulose degradation in the mutualism system of Odontotermes formosanus and associated microorganism[D]. Hangzhou: Zhejiang University, 2014.
[9] 刘佳佳, 嵇保中, 刘曙雯, 等. 南京地区黑翅土白蚁饵料筛选与取食记忆研究[J]. 林业科学研究, 2015, 28(1): 127-133. DOI:10.13275/j.cnki.lykxyj.2015.01.020 LIU J J, JI B Z, LIU S W, et al. Diets selection and feeding memory of Odontotermes formosanus in Nanjing[J]. Forest Research, 2015, 28(1): 127-133.
[10] 丁芳, 嵇保中, 刘曙雯, 等. 密粘褶菌松木粉培养物对黑翅土白蚁的生物活性[J]. 应用昆虫学报, 2015, 52(3): 736-742. DOI: 10.7679/j.issn.2095-1353.2015.086. DING F, JI B Z, LIU S W, et al. The biological activity of pine wood powder infected with Gloeophyllum trabeum on Odontotermes formosanus(Shiraki)[J]. Chinese Journal of Applied Entomology, 2015, 52(3): 736-742.
[11] 文平, 嵇保中, 刘曙雯, 等. 黑翅土白蚁踪迹信息素组分数量的柱层析分离和活性测定分析[J]. 应用昆虫学报, 2013, 50(6): 1622-1627. DOI: 10. 7679/j.issn.2095-1353. 2013. 223. WEN P, JI B Z, LIU S W, et al. Liquid chromatography and bioassay analysis of components in the trail pheromone of the black-wing subterranean termite Odontotermes formosanus(Shiraki)[J]. Chinese Journal of Applied Entomology, 2013, 50(6): 1622-1627.
[12] ESENTHER G R, ALLEN T C, CASIDA J E, et al. Termite attractant from fungus-infected wood[J]. Science, 1961, 134(347): 50. DOI: 10.1126/science.134.3471.50.
[13] OHMURA W, TOKORO M, TSUNODA K, et al. Termite trail-following substances produced by brown-rot fungi[J]. Material and Organismen. 1995, 29(2): 133-146.
[14] LEUTHOLD R H, LUSCHER M. An unusual caste polymorphism of the sternal gland and its trail pheromone production in the termite Trinervitermes bettonianus[J]. Insectes Sociaux, 1974, 21(4): 335-341. DOI: 10.1007/BF02331563.
[15] OLOO G W. The sternal gland: variation in size and activity in worker instars of Trinervitermes bettonianus (Sjöstedt)(Termitidae)[J]. Insect Science and its Application, 1981, 2(3): 145-147. DOI: 10.1017/S1742758400000928.
[16] SOLEYMANINEJADIAN E. Polyethism and its mechanism in Odontotermes formosanus(Shiraki)[D]. Nanjing: Nanjing Forestry University, 2014.
[17] 蔡邦华, 陈宁生, 陈安国, 等. 黑翅土白蚁Odontotermes formosanus (Shiraki)的蚁巢结构及其发展[J]. 昆虫学报, 1965, 14(1): 53-70. DOI:10.16380/j.kcxb.1965.01.007. CAI B H, CHEN N S, CHEN A G, et al. Architecture and development of the termitarium of Odontotermes formosanus(Shiraki)[J]. Acta Entomologica Sinica, 1965, 14(1): 53-70.
[18] WANG Z Y, MO J C, LU Y J. Biology and ecology of Macrotermes barneyi(Isoptera: Termitidae)[J]. Sociobiology, 2009, 54(3): 777-785. DOI:10.1007/s10618-007-0079-5.
[19] KIRKER G T, WAGNER T L, DIEHL S V. Relationship between wood-inhabiting fungi and Reticulitermes spp. in four forest habitats of northeastern Mississippi[J]. International Biodeterioration and Biodegradation, 2012, 72: 18-25. DOI: 10.1016/j.ibiod.2012.04.011.
[20] NGEE P S T, YOSHIMURA T, LEE C Y. Foraging populations and control strategies of subterranean termites in the urban environment, with special reference to baiting[J]. Japanese Journal of Environmental Entomology and Zoology, 2004, 15(3): 197-215.
[21] KASSENEY B D, DENG T, MO J. Effect of wood hardness and secondary compounds on feeding preference of Odontotermes formosanus(Isoptera: Termitidae)[J]. Journal of Economic Entomology, 2011, 104(3): 862-867. DOI: http://dx.doi.org/10.1603/EC10216.
[22] LITTLE N S, SCHULTZ T P, DIEHL S V, et al. Field evaluations of subterranean termite preference for sap-stain inoculated wood[J]. Journal of Insect Behavior, 2013, 26: 649-659. DOI: 10.1007/s10905-013-9380-x.
[23] 龙雁华. 高等培菌白蚁高效利用木质纤维素的机理研究[D]. 合肥: 安徽农业大学, 2009. LONG Y H. Study on mechanism of efficient utilization of lignocellulose by higher “fungus-growing” termite[D]. Hefei: Anhui Agricultural University, 2009.

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备注/Memo

备注/Memo:
基金项目:江苏高校优势学科建设工程资助项目(PAPD); 高等学校博士学科点专项科研基金项目(20123204110001); 江苏省自然科学基金项目(BK2012816); 南京市建委、中山陵园管理局资助项目(201409); 江苏省农业科技自主创新项目(CY(16)1005); 江苏省普通高校学术学位研究生科研创新计划项目(KYLX-150911) 第一作者:王亚召(775159143@qq.com)。*通信作者:嵇保中(jbz9885@njfu.edu.cn),教授,负责实验部分的指导工作。刘曙雯
更新日期/Last Update: 1900-01-01