[1]兰 月,辛昊阳,赵乙琏,等.小叶杨花粉母细胞减数分裂及花粉萌发力观测[J].南京林业大学学报(自然科学版),2017,41(06):177-180.[doi:10.3969/j.issn.1000-2006.201704060]
 LAN Yue,XIN Haoyang,ZHAO Yilian,et al.Study on meiosis of microspore mother cells and germination rate of pollen grains in Populus simonii Carr.[J].Journal of Nanjing Forestry University(Natural Science Edition),2017,41(06):177-180.[doi:10.3969/j.issn.1000-2006.201704060]
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小叶杨花粉母细胞减数分裂及花粉萌发力观测
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
41
期数:
2017年06期
页码:
177-180
栏目:
研究简报
出版日期:
2017-11-30

文章信息/Info

Title:
Study on meiosis of microspore mother cells and germination rate of pollen grains in Populus simonii Carr.
文章编号:
1000-2006(2017)06-0177-04
作者:
兰 月1辛昊阳1赵乙琏1孙志强2戴 筱1解志伟1席梦利1*
1.南京林业大学林学院,南方现代林业协同创新中心,江苏 南京 210037; 2.渭南职业技术学院,陕西 渭南 714000
Author(s):
LAN Yue1 XIN Haoyang1 ZHAO Yilian1 SUN Zhiqiang2 DAI Xiao1 XIE Zhiwei1 XI Mengli1*
1. Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China; 2. Weinan Vocational & Technical College,Weinan 714000, China
关键词:
小叶杨 小孢子母细胞 减数分裂 2n花粉
Keywords:
Keywords:Populus simonii Carr. microsporocyte meiosis 2n pollen
分类号:
S792.11
DOI:
10.3969/j.issn.1000-2006.201704060
文献标志码:
A
摘要:
【目的】探讨小叶杨(Populus simonii)小孢子母细胞减数分裂过程的特征及花粉萌发能力,为其杂交育种及分子细胞遗传学研究奠定基础。【方法】以小叶杨发育良好的雄花枝为研究材料,采用卡宝品红压片法,观察花粉母细胞减数分裂过程的特点,比较不同条件水培花枝对花粉萌发能力的影响。【结果】从小孢子母细胞发育到花粉粒,花芽除了稍增粗以外,外观没有明显变化; 减数分裂过程存在核仁数目的动态变化、落后染色体及不同类型的纺锤体; 室内水培花枝的花粉较室外水培的萌发能力强,室外水培的花枝花粉中观察到了2n花粉; 同一花枝上的不同花芽及同一花芽中不同小花的发育不同步,但同一小花中的不同花药发育基本一致。【结论】小叶杨可以产生天然2n花粉,是杨树多倍体育种及分子细胞遗传学研究的良好试验材料。
Abstract:
【Objective】In order to lay the foundation for cross breeding and molecular cytogenetics of Populus simonii, the characteristics of the meiosis processes of microsporocytes and pollen germination ability were analyzed.【Method】We observed the meiosis processes of microsporocytes using carbol fuchin staining, and compared the pollen germination capacity under different conditions for culturing flower branches.【Result】There was no obvious change in the flower buds except slightly thicker from the stage of microsporocyte to pollen grains during the meiosis. There was dynamic change of the number of nucleolus, lagged chromosomes and different kinds of spindles during the meiosis. The germination rate for the pollen grains of indoor flower branch is higher than the pollen grains of outdoor flower branch, and 2n gametes were observed from the pollen grains of outdoor flower branch. Different flower buds in one flowering branch or different florets in one flower bud showed non-synchronization in the meiosis processes of microsporocytes, but different anthers in one floret showed synchronization in the processes of meiosis. 【Conclusion】The P. simonii could produce natural 2n pollen. It is an ideal species for the research of polyploid breeding and molecular cytogenetics of poplar.

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相似文献/References:

[1]王影,黄敏仁,陈道明,等.杨树细胞悬浮培养及体细胞胚胎发生的研究[J].南京林业大学学报(自然科学版),1991,15(03):031.[doi:10.3969/j.jssn.1000-2006.1991.03.006]
 Wang Ying Huang Minren Chen Daoming Xu Nong.A STUDY ON CELL SUSPENSION CULTURE AND SOMATIC EMBRYOGENESIS OF POPULUS SIMONII[J].Journal of Nanjing Forestry University(Natural Science Edition),1991,15(06):031.[doi:10.3969/j.jssn.1000-2006.1991.03.006]
[2]辛昊阳,王 帅,刘光欣,等.美洲黑杨小孢子母细胞减数分裂进程与花芽及 花药外观形态相关性研究[J].南京林业大学学报(自然科学版),2016,40(02):048.[doi:10.3969/j.issn.1000-2006.2016.02.008]
 XIN Haoyang,WANG Shuai,LIU Guangxin,et al.Relationship between the meiosis processes of microsporocytes and morphology of male flower buds and anthers in Populus deltoides Marsh[J].Journal of Nanjing Forestry University(Natural Science Edition),2016,40(06):048.[doi:10.3969/j.issn.1000-2006.2016.02.008]

备注/Memo

备注/Memo:
基金项目:江苏省高校自然科学研究重大项目(16KJA220001); 国家自然科学基金项目(31670603); 江苏高校优势学科建设工程资助项目(PAPD) 第一作者:兰月(834648244@qq.com)。*通信作者:席梦利(ximenglinjfu@126.com),教授。
更新日期/Last Update: 1900-01-01