[1]陈 茜,范佳露,王宝腾,等.落叶型冬青雌、雄株茎段的组织培养技术[J].南京林业大学学报(自然科学版),2017,41(06):181-186.[doi:10.3969/j.issn.1000-2006.201704002]
 CHEN Xi,FAN Jialu,WANG Baoteng,et al.Micropropagation of Ilex verticillata axillary buds from male and female seedlings[J].Journal of Nanjing Forestry University(Natural Science Edition),2017,41(06):181-186.[doi:10.3969/j.issn.1000-2006.201704002]
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落叶型冬青雌、雄株茎段的组织培养技术
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
41
期数:
2017年06期
页码:
181-186
栏目:
研究简报
出版日期:
2017-11-30

文章信息/Info

Title:
Micropropagation of Ilex verticillata axillary buds from male and female seedlings
文章编号:
1000-2006(2017)06-0181-06
作者:
陈 茜12 范佳露13王宝腾13王瑞琪12 袁斌玲12魏子涵12陈 颖12*
1.南方现代林业协同创新中心,江苏 南京 210037; 2. 南京林业大学生物与环境学院, 江苏 南京 210037; 3. 南京林业大学林学院; 江苏 南京 210037
Author(s):
CHEN Xi12 FAN Jialu13 WANG Baoteng13 WANG Ruiqi12 YUAN Binling12 WEI Zihan12 CHEN Ying12*
1. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China; 2. College of Biology and Environment, Nanjing Forestry University; 3. College of Forestry, Nanjing Foresty University, Nanjing 210037, China
关键词:
落叶型冬青 组织培养 雌雄株 腋芽
Keywords:
Keywords:Ilex verticillata micropropagation male and female seedlings axillary buds
分类号:
S722.8
DOI:
10.3969/j.issn.1000-2006.201704002
文献标志码:
A
摘要:
【目的】建立落叶型冬青(Ilex verticillata L.)雌雄株的组织培养快繁体系。【方法】以落叶型冬青优良雌株和雄株的幼嫩茎段为材料, 通过添加不同浓度的6-BA、IBA、NAA、氯吡苯脲(CPPU)等植物生长调节剂,对外植体消毒时间、诱导培养基、增殖及生根培养基进行了优化。【结果】落叶型冬青雌株、雄株茎段外植体腋芽诱导最好的培养基分别为MS+0.5 mg/L 6-BA+0.2 mg/L IBA和MS+0.5 mg/L 6-BA+0.5 mg/L IBA,诱导率分别为93.3%和83.3%; 雌株和雄株丛芽增殖效果最好的培养基分别是MS+1.0 mg/L CPPU+0.2 mg/L NAA和MS+1.0 mg/L CPPU+0.1 mg/L NAA,增殖系数分别达3.48和1.25; 落叶型冬青在1/2 MS+0.4 mg/L NAA培养基上雌、雄株的生根率分别为26.7%和20.0%; 添加一种促根剂后,雌、雄株的生根率可达66.7%和46.7%。【结论】该组织培养体系特别是雄株组培体系的建立可为落叶型冬青苗木的快速繁殖提供技术支持。
Abstract:
【Objective】Establish a micropropagation system for male and female of Ilex verticillata L. plant(Winterberry).【Method】In this work, we optimized the culture mediums of axillary bud induction, clustered bud proliferation and shoot rooting by using semi-lignied buds of male and female seedling and adding different regulators such as 6-benzylaminopurine(6-BA), indole-3-butyric acid(IBA), naphthaleneacetic acid(NAA), and N-(2-chloro-4-pyridyl)-N'-phenylurea(CPPU)in vitro.【Result】The optimal method for sterilization of explants was treatment with 70% alcohol for 30 s, and 0.1% HgCl2 for 8 min; the optimal media for axillary bud induction of female and male explants were MS+0.5 mg/L 6-BA+0.2 mg/L IBA and MS + 0.5 mg/L 6-BA + 0.5 mg/L IBA, and this treatment resulted in axillary bud germination rates up to 93.3% and 83.3%, respectively. Furthermore, the optimal media for female and male multiple shoot clumps were MS + 1.0 mg/L CPPU + 0.2 mg/L NAA and MS + 1.0 mg/L CPPU + 0.1 mg/L NAA, this treatment resulted in proliferation rates up to 3.48 and 1.25, respectively. The shoots were induced to form roots on a 1/2MS + 0.4 mg/L NAA medium, and the rooting rates of females and males were only 26.7% and 20.0%, which increased to 66.7% and 46.7%, respectively, when a rooting agent was added to the medium. 【Conclusion】The establishment of this tissue culture system, especially the male plant tissue culture, provides technical support for the rapid propagation of Ilex verticillata species.

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备注/Memo

备注/Memo:
基金项目:国家林业局“948”项目(2014-4-61); 江苏高校优势学科建设工程资助项目(PAPD); 南京林业大学大学生创新训练计划项目(2016NFUSPITP002)。 第一作者:陈茜(948092138@qq.com)完成了试验设计及论文写作; 范佳露(987506225@qq.com)完成了试验操作及论文写作及修改。*通信作者:陈颖(chynjfu@163.com),教授。
更新日期/Last Update: 1900-01-01