[1]吴秋芳,胡海波*,张 鑫.黑曲霉及其代谢产物对花岗岩风化作用的影响[J].南京林业大学学报(自然科学版),2018,42(01):081-88.[doi:10.3969/j.issn.1000-2006.201610049 ]
 WU Qiufang,HU Haibo*,ZHANG Xin.Effect of Aspergillus niger and its metabolites on weathering of granite[J].Journal of Nanjing Forestry University(Natural Science Edition),2018,42(01):081-88.[doi:10.3969/j.issn.1000-2006.201610049 ]
点击复制

黑曲霉及其代谢产物对花岗岩风化作用的影响
分享到:

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
42
期数:
2018年01期
页码:
081-88
栏目:
研究论文
出版日期:
2018-01-31

文章信息/Info

Title:
Effect of Aspergillus niger and its metabolites on weathering of granite
文章编号:
1000-2006(2018)01-0081-07
作者:
吴秋芳12胡海波1*张 鑫1
1.南方现代林业协同创新中心,南京林业大学,江苏 南京 210037; 2.安阳工学院生物与食品工程学院,河南 安阳 455000
Author(s):
WU Qiufang12 HU Haibo1* ZHANG Xin1
1. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China; 2. College of Biology and Food Engineering, Anyang Institute of Technology, Anyang 455000, China
关键词:
黑曲霉 风化 花岗岩 有机酸 氨基酸 多糖
Keywords:
Keywords:Aspergillus niger weathering granite organic acid amino acid polysaccharide
分类号:
S151; Q938
DOI:
10.3969/j.issn.1000-2006.201610049
文献标志码:
A
摘要:
【目的】为揭示微生物风化岩石的机理,促进土壤的形成和岩石边坡覆绿,改善生态环境,笔者研究了黑曲霉(Aspergillus niger)及代谢产物对花岗岩风化作用的影响。【方法】从花岗岩风化土壤中分离、纯化得到一株高效风化黑曲霉菌株,采用摇瓶实验和电镜分析,研究黑曲霉在发酵过程中产生代谢产物的种类及其对花岗岩中硅、铁、铝、钠等元素的浸出效果。【结果】在含有花岗岩粉的培养基中,黑曲霉发酵液中合成的6种有机酸(草酸、酒石酸、琥珀酸、苹果酸、乙酸、柠檬酸)的含量在发酵初期逐渐升高,在发酵后期随着发酵时间的延长而逐渐降低; 在不同发酵期合成的氨基酸种类不同。含花岗岩粉的培养基有利于黑曲霉大量合成多糖类物质。黑曲霉代谢产物摇瓶实验表明,有机酸、氨基酸、多糖等均可破坏岩石结构,并释放出硅、铁、铝、钠等元素。在岩石风化时,代谢产物具有协同作用,黑曲霉风化花岗岩效果最优,有机酸、氨基酸、多糖混合物浸出元素的效果又明显优于它们各自单独作用时的效果。【结论】黑曲霉及其代谢产物有机酸、氨基酸、多糖以及三者混合物都具有风化岩石的作用,且黑曲霉风化岩石的效果优于单一和混合代谢产物。
Abstract:
【Objective】To reveal the mechanism of microbial weathering of rocks, promote the rock slope to covered with plants, and improve the ecosystem, the effect of Aspergillus niger and its metabolites on the weathering of granite were studied in this research.【Method】An A. niger strain, which is highly efficient in weathering rocks, was isolated and purified from the granite soil. Liquid dynamic culture and electron microscopic analysis were used to study the metabolites produced by A. niger during the fermentation. Effects of the metabolites on leaching of silicon, iron, aluminum, and other elements from granite were studied.【Result】The results indicated that A. niger in the medium containing granite powder can synthesize six kinds of organic acids, including oxalic acid, tartaric acid, succinic acid, malic acid, acetic acid, and citric acid in its fermentation liquors. The contents of these organic acids gradually increased at early stage of fermentation, and then decreased with the fermentation process. Which indicated that A. niger could uptake and utilization those organic acids during growth and reproduction. A. niger synthesizes different amino acids in different fermentation stages, and the medium containing granite powder is beneficial in synthesizing polysaccharides. The results of metabolites of A. niger in liquid dynamic culture indicate that organic acid, amino acid, and polysaccharides can destroy the structure of rocks and release silicon, iron, aluminum, sodium, and other elements from granite. During rock weathering, the metabolites have certain synergistic effect. The effect of the mixture of organic acids, amino acids, and polysaccharides on elements extractions is significantly better than their respective effects.【Conclusion】A. niger and its metabolites, including organic acids, amino acids, and polysaccharides, and their mixture facilitate the weathering of granite, and the effect of weathering granite by A.niger is superior to that of single and mixed metabolites.

参考文献/References:

[1] 杨金玲,张甘霖,黄来明.典型亚热带花岗岩地区森林流域岩石风化和土壤形成速率研究[J]. 土壤学报, 2013, 50(2): 253-259. DOI: 10.11766/trxb201204120128. YANG J L, ZHANG G L, HUANG L M. Rock weathering and soil formation rates of a forested watershed in the typical subtropical granite area[J]. Acta Pedologica Sinica, 2013, 50(2):253-259.
[2] 陈骏,姚素平.地质微生物学及其发展方向[J].高校地质学报,2005, 11(2): 154-166. DOI: 10.3969/j.issn.1006-7493.2005.02.002. CHEN J, YAO S P. Geomicrobiology and its progress[J].Geological Journal of China Universities, 2005, 11(2): 154-166.
[3] 成玉祥,段玉贵,李格烨,等.岩石冻融风化作用积累泥石流物源试验研究[J].灾害学,2015, 30(2): 46-50. DOI: 10.3969/j.issn.1000-811X.2015.02.009. CHENG Y X, DUAN Y G, LI G Y, et al. The study on debris flows material source accumulation by rock freezing and thawing weathering test[J]. Journal of Catastrophology, 2015, 30(2): 46-50.
[4] 杜叶,周雪莹,连宾.胶质芽孢杆菌的胞外分泌物与细菌的解钾作用[J].地学前缘, 2008, 15(6): 107-111. DOI: 10.3321/j.issn:1005-2321.2008.06.014. DU Y, ZHOU X Y, LIAN B. The extracellular secretion of Bacillus mucilaginosus and capabillty of releasing potassium from potassium-bearing minerals[J]. Earth Science Frontiers, 2008, 15(6): 107-111.
[5] 李福春,李莎,杨用钊,等.原生硅酸盐矿物风化产物的研究进展——以云母和长石为例[J].岩石矿物学杂志,2006, 25(5): 440-448. LI F C, LI S, YANG Y Z, et al. Advances in the study of weathering products of primary siliate minerals, exemplified by mica and feldspar [J]. Acta Petrologica ET Mineralogica,2006, 25(5): 440-448.
[6] 李涵,蔡林,姚奇志,等.真菌的生物地质作用及其在环境修复中的应用[J].高校地质学报,2015, 21(3): 383-394. DOI: 10.16108/j.issn1006-7493.2015169. LI H, CAI L, YAO Q Z, et al. Fungal involvement in biogeological processes and application to environmental bioremediation[J].Geological Journal China Universities, 2015, 21(3): 383-394.
[7] 连宾,陈烨,朱立军.等.微生物对碳酸盐岩的风化作用[J].地质前缘, 2008, 15(6): 90-99. DOI: 10.3321/j.issn:1005-2321.2008.06.012. LIAN B,CHEN Y, ZHU L J, et al. Progress in the study of the weathering of carbonate rock by microbes[J]. Earth Science Frontiers, 2008, 15(6): 90-99.
[8] 胡海波,魏勇,仇才楼.苏北沿海防护林土壤可蚀性的研究[J].水土保持研究, 2001, 8(1): 150-154. DOI: 10.3969/j.issn.1005-3409.2001.01.033. HU H B, WEI Y, QIU C L. Study on soil erodibility of shelter-forest in silting coastal area of northern Jiangsu Province[J]. Research of Soil and Water Conservation, 2001, 8(1): 150-154.
[9] 薛建辉,吴永波,方升佐.退耕还林工程困难立地植被恢复与生态重建[J].南京林业大学学报(自然科学版), 2003, 27(6): 84-88. DOI: 10.3969/j.issn.1000-2006.2003.06.021. XUE J H, WU Y B, FANG S Z. A study on the vegetation restoration and ecological reconstruction in degraded sites of forest rehabilitation areas[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2003, 27(6): 84-88.
[10] 吴雁雯,张金池.微生物碳酸酐酶在岩溶系统循环中的作用与应用研究进展[J].生物学杂志, 2015, 32(3): 78-83. DOI: 10. 3969/j.issn. 2095-1736. 2015. 03. 078. WU Y W, ZHANG J C. Microbial carbonic anhydrase action and application on carbon cycling in Karst dynamic system:a review[J]. Journal of Biology, 2015, 32(3): 78-83.
[11] 单奇华,李卫正,俞元春,等. 南京城市林业土壤可蚀性及影响因素[J].南京林业大学学报(自然科学版), 2008, 32(2): 47-50. DOI: 10.3969/j.issn.1000-2006.2008.02.010. SHAN Q H, LI W Z, YU Y C, et al. Erodibility of urban forest soil in Nanjing[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2008, 32(2): 47-50.
[12] 孙蕾蕾,肖雷雷,肖波,等.黑曲霉风化含钾岩石过程中碳酸酐酸和半胱氨酸合成酶基因表达量的差异[J].中国科学:地球科学,2013, 43(11): 1828-1833. DOI: 10.1007/S11430-013-4704-4. SUN L L, XIAO L L, XIAO B, et al. Differences in the gene expressive quantities of carbonic anhydrase and cysteine synthase in the weathering of potassium-bearing minerals by Aspergillus niger[J]. Science China: Earth Sciences, 2013, 43(11): 1828-1833.
[13] 董翠玲,连宾.细菌与真菌对黑云母的风化作用比较——以胶质芽孢杆菌和黑曲霉为例[J].矿物岩石地球化学通报, 2014, 33(6): 772-777. DOI: 10.3969/j.issn.1007-2802.2014.06.003. DONG C L, LIAN B. Comparing the bio-weathering effects for biotite by Bacillus mucilaginosus and Aspergillus niger[J]. Bulletin of Mineralogy, Petrology and Geochemistry, 2014, 33(6): 772-777.
[14] 胡婕,郁建平,连宾,等.黑曲霉对含钾矿物的解钾作用与机理分析[J].矿物岩石地球化学通报, 2011,30(3): 277-285. DOI: 10.3969/j.issn.1007-2802.2011.03.006. HU J, YU J P, LIAN B, et al. Capability and mechanism of potassium releasing frompotassium-bearing minerals by Aspergillus niger[J]. Bulletin of Mineralogy, Petrology and Geochemistry, 2011, 30(3): 277-285.
[15] 孙德四,万谦,赵薪萍,等.胶质芽孢杆菌JXF菌株代谢产物与脱硅作用研究[J].矿业工程, 2008, 28(3): 52-56. DOI: 10.3969/j.issn.0253-6099.2008.03.014. SUN D S, WAN Q, ZHAO X P. Metabolites of Bacillus mucilaginous JXF and their desiliconization effect[J]. Mining and Metallurgicaleng Ineering, 2008, 28(3): 52-56.
[16] 王秀林,孙德四,曹飞,等.硅酸盐细菌代谢产物对不同结构硅酸盐矿物风化作用的影响[J].非金属矿, 2013, 36(1): 1-4. DOI: 10.3969/j.issn.1000-8098.2013.01.003. WANG X L, SUN D S, CAO F, et al. The influence of silicate bacterial metabolites on weathering of silicate minerals with different crystal structures[J]. Non-Metallic Mines, 2013, 36(1): 1-4.
[17] 孙德四,张贤珍,张强.硅酸盐细菌代谢产物对硅酸盐矿物的浸溶作用研究[J]. 矿冶工程, 2006, 26(3): 39-42. DOI: 10.3969/j.issn.0253-6099.2006.03.008. SUN D S, ZHANG X Z, ZHANG Q. Leaching effects of metabolites of silicate bacterium on silicate minerals[J]. Mining and Metallurgicaleng Ineering, 2006, 26(3): 39-42.
[18] 肖国光,孙德四,曹飞.硅酸盐细菌代谢产物影响斜长石风化作用的模拟试验[J].矿物岩石, 2013, 33(3): 8-15. XIAO G G, SUN D S, CAO F. Wearthering of silicate minerals by metabolites produced by siliccate bacteria in culture experiments[J]. Mineral Petrol, 2013, 33(3): 8-15.
[19] TYRIAKOVÁ I, TYRIAK I, OBERHÄNSLI H. Rock weathering by indigenous heterotrophic bacteria of Bacillus spp. at different temperature:a laboratory experiment[J]. Miner Petro, 2012, 105:135-144. DOI:10.1007/s00710-012-0201-2.
[20] KEVIN H, JOSELITO M A, JAN B, et al. The influence of aspect on the biological weathering of granites:observations from the Kunlun Mountain China[J]. Geomorphology, 2005, 67: 171-188. DOI: 10.1016/j.geomorph.2004.09.027.

相似文献/References:

[1]宋向阳,方浩,欧阳嘉,等.液态混合菌发酵优化纤维素酶组分[J].南京林业大学学报(自然科学版),2011,35(02):078.[doi:10.3969/j.jssn.1000-2006.2011.02.017]
 SONG Xiangyang,FANG Hao,OUYANG Jia,et al.ptimization of cellulase components by mixed culture of Trichoderma reesei and Aspergillus niger[J].Journal of Nanjing Forestry University(Natural Science Edition),2011,35(01):078.[doi:10.3969/j.jssn.1000-2006.2011.02.017]
[2]宋娜娜,宋向阳*,连之娜,等.纤维素酶液中β-葡萄糖苷酶的分离纯化[J].南京林业大学学报(自然科学版),2011,35(04):111.[doi:10.3969/j.jssn.1000-2006.2011.04.023]
 SONG Nana,SONG Xiangyang*,LIAN Zhina,et al.Isolation and purification of β-glucosidase from cellulase solution[J].Journal of Nanjing Forestry University(Natural Science Edition),2011,35(01):111.[doi:10.3969/j.jssn.1000-2006.2011.04.023]
[3]赵林果,游丽金,倪 浩,等.碳源和氮源对黑曲霉分泌β-葡萄糖苷酶的影响[J].南京林业大学学报(自然科学版),2008,32(06):001.[doi:10.3969/j.jssn.1000-2006.2008.06.001]
 ZHAO Lin-guo,YOU Li-jin,NI Hao,et al.Effects of carbon and nitrogen sources on β- glucosidase biosynthesis by Aspergillus niger nl -1[J].Journal of Nanjing Forestry University(Natural Science Edition),2008,32(01):001.[doi:10.3969/j.jssn.1000-2006.2008.06.001]
[4]张晓燕,李 鑫,王 静,等.碳源和氮源对固态发酵制备β-甘露聚糖酶的影响[J].南京林业大学学报(自然科学版),2015,39(04):143.[doi:10.3969/j.issn.1000-2006.2015.04.025]
 ZHANG Xiaoyan,LI Xin,WANG Jing,et al.Effects of carbon source and nitrogen source on solid state fermentation for the production of β-mannanase[J].Journal of Nanjing Forestry University(Natural Science Edition),2015,39(01):143.[doi:10.3969/j.issn.1000-2006.2015.04.025]

备注/Memo

备注/Memo:
基金项目:江苏省林业“三新”工程项目(lysx[2013] 10); 长江三角洲森林生态系统定位研究项目; 江苏高校优势学科建设工程资助项目(PAPD); 江苏省水土保持与生态修复重点实验室开放项目(2016); 安阳工学院校级基金项目(YJJ2015011) 第一作者:吴秋芳(410972369@qq.com)。*通信作者:胡海波(huhb2000@aliyun.com),教授。
更新日期/Last Update: 2018-03-30