[1]安常蓉,韦小丽*,段如雁,等.接种方式对花榈木幼苗结瘤效应的影响[J].南京林业大学学报(自然科学版),2018,42(02):009-16.[doi:10.3969/j.issn.1000-2006.201705049]
 AN Changrong,WEI Xiaoli*,DUAN Ruyan,et al.Effects of inoculation ways on nodule formations of precious timber tree species Ormosia henryi seedlings[J].Journal of Nanjing Forestry University(Natural Science Edition),2018,42(02):009-16.[doi:10.3969/j.issn.1000-2006.201705049]
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接种方式对花榈木幼苗结瘤效应的影响
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
42
期数:
2018年02期
页码:
009-16
栏目:
研究论文
出版日期:
2018-03-20

文章信息/Info

Title:
Effects of inoculation ways on nodule formations of precious timber tree species Ormosia henryi seedlings
文章编号:
1000-2006(2018)02-0009-08
作者:
安常蓉1 韦小丽1* 段如雁12 叶 润1 黎星炜1
1.贵州大学林学院,贵州 贵阳 550025; 2.贵州省生物研究所,贵州 贵阳 550009
Author(s):
AN Changrong1 WEI Xiaoli1* DUAN Ruyan12 YE Run1 LI Xingwei1
1.Forestry College of Guizhou University, Guiyang 550025, China; 2.Guizhou Institute of Biology,Guiyang 550009, China
关键词:
花榈木幼苗 接种方式 结瘤 生长 生理生化 固氮
Keywords:
Keywords:Ormosia henryi seedlings inoculation way nodules growth physiology and biochemy nitrogen fixation
分类号:
S718.8; S723.1
DOI:
10.3969/j.issn.1000-2006.201705049
文献标志码:
A
摘要:
【目的】探讨珍贵用材树种花榈木幼苗接种根瘤菌的有效方法。【方法】设计了浸种、浸根、拌种和浇灌4种接种方式进行盆栽试验,以不接根瘤菌的盆栽苗为对照,观察接种后幼苗根系形态、结瘤数量变化,并测定幼苗生长、生理生化指标及固氮相关指标。【结果】不同接种方式处理下花榈木幼苗根系松软、呈土黄色,幼瘤多呈球形、黄褐色,结瘤部位多位于侧须根系上。不同接种方式处理的花榈木幼苗结瘤数量和质量差异明显,浇灌处理的结瘤数量最多,鲜瘤生物量最大,结瘤率最高,其次是浸根处理。接种上根瘤的花榈木幼苗生长指标均大于CK,与CK相比,4种接种方式处理下的苗高、地径和总生物量增幅分别为11.48%~29.51%、3.21%~26.61%和36.30%~148.18%; 总根长、总根表面积、根平均直径、总根体积和根尖数增幅分别为70.62%~139.39%、74.70%~140.97%、164.28%~200.00%、75.00%~250.00%和28.03%~167.42%。不同接种方式处理的花榈木结瘤幼苗生理生化指标差异显著,浇灌处理的花榈木结瘤幼苗叶绿素含量、硝酸还原酶活性、硝态氮含量和根系活力都明显高于其他处理。接种上根瘤的花榈木幼苗叶片全氮、鲜瘤豆血红蛋白含量、总氮量以及固氮量明显高于CK,浇灌和浸根处理优于其他处理。【结论】花榈木根瘤接种的适宜方式为用萌发种子浸根和用菌液浇灌长出真叶后的幼苗。
Abstract:
【Objective】The aim of this study was to explore the effects of rhizobium inoculation methods on Ormosia henryi seedlings.【Method】We designed a potting test that included four inoculation methods: soaking the seed, soaking the radicle, mixing rhizobium liquid with the seed, and pouring rhizobium liquid, which non-inoculated rhizobium as a control. We observed the root morphology and quantity of nodules, and determined some indexes that relate to growth, physiology, and biochemistry as well as nitrogen fixation. 【Result】The result showed that the roots of the seedlings were loose, soft and appear khaki, most of the young nodules are spherical and appear yellowish-brow, and most nodules grew from the lateral root. The quantity and quality of nodules were significantly different among the different inoculation methods; the seedlings inoculated by pouring rhizobium showed a higher number and weight of nodules and a higher nodulation rate than those by other methods, and the seedlings inoculated by soaking the radicle were second highest. The growth indices of Ormosia henryi seedlings with nodules were bigger than that of the control. Compared to the control, the height, ground diameter, and biomass of seedlings treated by four inoculation methods increased by 11.48%-29.51%, 3.21%-26.61% and 36.30%-148.18%, respectively; the total root length, total root surface area, average root diameter, total root volume, and root tip number increased by 70.62%-139.39%, 74.70%-140.97%, 164.28%-200.00%, 75.00%-250.00% and 28.03%-167.42%, respectively. The physiological and biochemical indices of seedlings with nodules by different inoculation methods showed significant difference. Under the pouring rhizobium method, the chlorophyll content, nitrate nitrogen content, nitrate reductase activity, and root activity were significantly higher than those seen in other treatments. The total nitrogen content, hemoglobin content, total nitrogen content, and nitrogen fixation rate of inoculated seedling leaves were significantly higher than those without inoculated rhizobium, and the nitrogen fixation effect in the seedlings inoculated by pouring rhizobium liquid and soaking the radicle were higher than those in the other treatment groups.【Conclusion】The most suitable methods of inoculation are soaking the roots of germination seeds and irrigating the Ormosia henryi seedlings using rhizobium liquid when nodules grow on the seedlings.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(31460193); 贵州省高层次创新人才培养计划项目(2006-1); 贵州省林业厅重大攻关项目(黔林科合[2010]重大02) 第一作者:安常蓉(1522281316@qq.com)。*通信作者:韦小丽(gdwxl-69@126.com),教授。
更新日期/Last Update: 2018-06-12