[1]兖 攀,彭方仁*,张 瑞,等.美国山核桃嫁接愈合过程的组织细胞学观察[J].南京林业大学学报(自然科学版),2018,42(02):202-206.[doi:10.3969/j.issn.1000-2006.201703067]
 YAN Pan,PENG Fangren*,ZHANG Rui,et al.Histo-cytological observation on the graft union formation in Carya illinoinensis[J].Journal of Nanjing Forestry University(Natural Science Edition),2018,42(02):202-206.[doi:10.3969/j.issn.1000-2006.201703067]
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美国山核桃嫁接愈合过程的组织细胞学观察
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
42
期数:
2018年02期
页码:
202-206
栏目:
研究简报
出版日期:
2018-03-20

文章信息/Info

Title:
Histo-cytological observation on the graft union formation in Carya illinoinensis
文章编号:
1000-2006(2018)02-0202-05
作者:
兖 攀1彭方仁1*张 瑞1曹明闽1翟 敏2刘壮壮1
1 南京林业大学林学院,江苏 南京 210037; 2 南京绿宙薄壳山核桃科技有限公司,江苏 南京 210007
Author(s):
YAN Pan1 PENG Fangren1* ZHANG Rui1 CAO Mingmin1 ZHAI Min2 LIU Zhuangzhuang1
1. College of Forestry, Nanjing Forestry University, Nanjing 210037,China; 2. Nanjing Lüzhou Pecan Technology Company,Nanjing 210007, China
关键词:
美国山核桃 嫁接愈合 愈伤组织 显微结构
Keywords:
Keywords:Carya illinoinensis graft union callus microstructure
分类号:
S644; Q944.55
DOI:
10.3969/j.issn.1000-2006.201703067
文献标志码:
A
摘要:
【目的】揭示美国山核桃(Carya illinoinensis)嫁接愈合过程的组织细胞学特征。【方法】以2年生‘马罕'实生苗为砧木,接穗取自‘波尼'品种的1年生枝条,采用切接法进行嫁接,对其嫁接愈合过程进行了组织细胞学观察。【结果】美国山核桃的嫁接愈合过程历经砧穗隔离期(1~9 d)、愈伤组织生长期(9~20 d)、形成层环构成期(20~25 d)和新生维管组织分化期(25~40 d)4个时期。嫁接后1个月左右形成愈伤组织桥和形成层环,为嫁接成活的关键期; 在未成活的嫁接体中,愈伤组织细胞较少并褐化,不能完成愈合过程。【结论】美国山核桃接穗愈伤组织大量发生的时期早于砧木,愈伤组织大量发生期与叶片展开在同一时期; 愈伤组织主要在形成层处产生; 愈伤组织生长受阻,嫁接愈合过程中断可能是影响嫁接成活的重要内因。因此,为愈伤组织的生长与形成层环的构成创造有利条件,是嫁接技术改进、提高嫁接成活率的重要途径。
Abstract:
【Objective】The purpose of this study was to reveal the histo-cytic characteristics of pecan(Carya illinoinensis)during the process of graft union. 【Method】One-year-old scions of the ‘Pawnee' cultivar were grafted on the two-year-old seedlings of ‘Mahan' by cut grafting, and a histo-cytological observation was performed during the process of graft union. 【Result】Four periods in the process of graft union was observed, including the isolation period of scion and rootstock(1-9 d), callus growth period(9-20 d), cambium ring formation period(20-25 d), and new vascular tissue differentiation period(25-40 d). The callus bridge and cambium ring were formed one month after grafting, which was assumed to be the critical period of graft survival. The union cannot be completed in the dead grafts, in which callus cells were less and browning.【Conclusion】Large amounts of callus occurred in scions earlier than that in rootstocks and the leaves unfolded at the stage when the large amounts of callus occurred; the callus mainly formed in cambium; the inhibition of the growth of callus and the interruption of the process of graft union may be the important factor influencing grafting survival. To improve grafting techniques and grafting survival rate, it appears important that the conditions need to be explored to promote the growth of the callus and the formation of cambium ring.

参考文献/References:

[1] 李永荣, 吴文龙, 刘永芝. 薄壳山核桃种质资源的开发利用[J]. 安徽农业科学, 2009, 37(27): 13306-13308. LI Y R, WU W L, LIU Y Z. Development and utilization of pecan germplasm resources[J]. Journal of Anhui Agricultural Sciences, 2009, 37(27): 13306-13308.
[2] 彭方仁, 李永荣, 郝明灼, 等. 我国薄壳山核桃生产现状与产业化发展策略[J]. 林业科技开发, 2012, 26(4): 1-4. DOI: 10.3969/j.issn.1000-8101.2012.04.001. PENG F R, LI Y R, HAO M Z, et al. The production status and development strategy for pecan industry in China[J]. Journal of Forestry Engineering, 2012, 26(4): 1-4.
[3] 韩杰,张明,林苏宝,等.不同修剪措施对薄壳山核桃幼树光合特性的影响[J].南京林业大学学报(自然科学版),2017,41(6):13-18.DOI:10.3969/j.issn.1000-2006.201703024. HAN J, ZHANG M, LIN S B, et al. Effects of different pruning measures on the photosynthetic characteristics of pecan seedlings[J].Journal of Nanjing Forestry University(Natural Sciences Edition),2017,41(6):13-18.
[4] 夏根清, 翁春余, 王开良, 等. 薄壳山核桃嫁接技术试验[J]. 经济林研究, 2007, 25(4): 109-112. DOI: 10.3969/j.issn.1003-8981.2007.04.024 XIA G Q, WENG C Y, WANG K L, et al. Grafting Techniques in Carya illinoensis[J]. Nonwood Forest Research, 2007, 25(4): 109-112.
[5] AJAMGARD F, RAHEMI M, VAHDATI K. Development of improved techniques for grafting of pecan[J]. Scientia Horticulturae, 2016, 204:65-69. DOI: 10.1016/j.scienta.2016.03.030.
[6] 李和平. 植物显微技术[M]. 北京: 科学出版社, 2009.
[7] 叶宝兴, 毕建杰, 孙印石. 植物细胞与组织研究方法[M]. 北京:化学工业出版社, 2011.
[8] 曹建华, 林位夫, 陈俊明. 砧木与接穗嫁接亲合力研究综述[J]. 热带农业科学, 2005, 25(4): 64-69. DOI:10.3969/j.issn.1009-2196.2005.04.018. CAO J H, LIN W F, CHEN J M. Studies of affinity between rootstock and scion[J]. Chinese Journal of Tropical Agriculture, 2005, 25(4): 64-69.
[9] 丁平海, 郗荣庭. 核桃枝接愈合过程的解剖学观察[J]. 林业科学, 1991, 27(4): 457-460. DING P H, XI R T.The anatomical observation for the process of the graft union by scion grafting in walnut[J]. Scientia Silvae Sinicae, 1991, 27(4): 457-460.
[10] 刘传荷. 山核桃嫁接愈合过程的解剖学研究及IAA免疫金定位[D]. 临安: 浙江林学院, 2008. LIU C H. Anatomical changes and immunogold localization of IAA in cells of hickory's graft union[J]. Lin'an: Zhejiang Forestry University, 2008.
[11] 赵红玲. 葡萄嫁接愈合过程研究及嫁接对葡萄生长发育、产量和品质的影响[D]. 长春: 吉林农业大学, 2004. ZHAO H L. Studies on the process of graft union and its effects on the development, yield and qualities of grapes[D]. Changchun: Jilin Agricultural University, 2004.
[12] 初庆刚, 张长胜. 梨树嫁接愈合的解剖观察[J]. 莱阳农学院学报, 1992, 9(4): 256-259. CHU Q G, ZHANG C S. The anatomical observation for the graft union in pear tree[J]. Journal of Laiyang Agricultural College, 1992, 9(4): 256-259.
[13] PINA A, ERRE A P.A review of new advances in mechanism of graft compatibility-incompatibility[J].Scientia Horticulturae,2005, 106: 1-11. DOI:10.1016/j.scienta.2005.04.003.
[14] FAN J W, YANG R, LI X M, et al. The processes of graft union formation in tomato[J].Horticulture Environment & Biotechnology, 2015, 56(5): 569-574. DOI: 10.1007/s13580-015-0009-1.
[15] MOORE R, WALKER D B. Studies of vegetative compatibility-incompatibility in higher plants[J]. Protoplasma, 1981, 109(3/4): 317-334. DOI: 10.1007/bf01279803.
[16] MILLER H, BARENTT J. The structure and composition of bead-like projections on sitka spruce callus cells formed during grafting and in culture[J]. Annals of Botany, 1993, 72(5): 441-448. DOI: 10.1006/anbo.1993.1130.
[17] EBERHARD S, DOUBRAVA N, MARFA V, et al. Pectic cell wall fragments regulate tobacco thin-cell-layer explant morphogenesis[J].Plant Cell,1989,1(8):747-755. DOI: 10.1105/tpc.1.8.747.
[18] MOHNEN D, EBERGARD S, MARFA V, et al. The control of root, vegetative shoot and flower morpho genesis in tobacco thin cell-layer explants(TCLs)[J]. Development, 1990, 108(1): 191-201.
[19] 杨世杰. Impatiens walleriana/Impatiens olivieri嫁接过程的组织学和细胞学观察[J]. 中国农业大学学报,1987,8(3):359-366. YANG S J. Observation on histological and cellular events of interspecific grafting(Impatiens walleriana/Impatiens olivieri)[J]. Journal of China Agricultural University, 1987,8(3):359-366.
[20] 杨世杰, 卢善发. 植物嫁接基础理论研究[J]. 生物学通报, 1995, 30(10): 4-6. YANG S J, LU S F. Basic theory research on plant grafting[J]. Bulletin of Biology, 1995, 30(10): 4-6.
[21] 韦存虚, 蓝盛银, 徐珍秀. 水稻胚乳细胞质膜内陷的超微结构和磷酸酶的细胞化学定位[J]. 植物生理与分子生物学学报, 2002, 28(3): 221-226. DOI:10.3321/j.issn:1671-3877.2002.03.011. WEI C X, LAN S Y, XU Z X. The ultrastructure and cytochemical localization of phosphatase activity of plasmalemma invagination in endosperm cells of rice[J]. Journal of Plant Physiology and Molecular Biology, 2002, 28(3): 221-226.
[22] TIEDEMANN R. Graft union development and symplastic phloem contact in the heterograft cucumissativus on Cucurbita ficifolia [J]. Journal of Plant Physiology, 1989, 134(4): 427-440. DOI: 10.1016/s0176-1617(89)80006-9.
[23] YIN H, YAN B, SUN J, et al. Graft-union development: a delicate process that involves cell-cell communication between scion and stock for local auxin accumulation[J]. Journal of Experimental Botany, 2012, 63(11): 4219-32. DOI: 10.1093/jxb/ers109.
[24] GAINZA F, OPAZO I, MUNOZ C. Graft incompatibility in plants: metabolic changes during formation and establishment of the rootstock/scion union with emphasis on Prunus species[J]. Chilean Journal of Agricultural Research, 2015, 75(1): 20-31. DOI: 10.4067/s0718-58392015000300004.
[25] HUDINA M, ORAZEM P, JAKOPIC J, et al. Scion-Rootstock interaction affects the phytochemical composition of Prunus phloem[J]. European Journal of Horticultural Science, 2014, 79(5): 248-259.
[26] 莫正海,何海洋,陈文静,等.薄壳山核桃嫁接愈合过程中差异蛋白质的分析[J].南京林业大学学报(自然科学版),2017,41(6): 19-25.DOI:10.3969/j.issn.1000-2006.201703021. MO Z H, HE H Y, CHEN W J, et al. Analysis of the differential proteins in the graft healing process of pecan(Carya illinoinensis)[J].Journal of Nanjing Forestry University(Natural Sciences Edition),2017,41(6):19-25.
[27] STEGEMANN S, BOCK R. Exchange of genetic material between cells in plant tissue grafts[J]. Science, 2009, 324(5927): 649-651. DOI: 10.1126/science.1170397.

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备注/Memo

备注/Memo:
基金项目:国家林业局“948”项目(2015-4-16); 江苏省林业“三新”工程项目(Lysx[2016]44); 江苏高校优势学科建设工程资助项目(PAPD) 第一作者:兖攀(yanpan198687@163.com),工程师。*通信作者:彭方仁(frpeng@ njfu.edu.cn),教授。
更新日期/Last Update: 2018-06-12