[1]马迎莉,时 蕾,张雨峰,等.萘乙酸对太子参生长及其块根有效成分的影响[J].南京林业大学学报(自然科学版),2018,42(03):123-130.[doi:10.3969/j.issn.1000-2006.201701014]
 MA Yingli,SHI Lei,ZHANG Yufeng,et al.Effects of NAA on growth and active ingredients in root tuber of Pseudostellaria heterophylla[J].Journal of Nanjing Forestry University(Natural Science Edition),2018,42(03):123-130.[doi:10.3969/j.issn.1000-2006.201701014]





Effects of NAA on growth and active ingredients in root tuber of Pseudostellaria heterophylla
马迎莉时 蕾张雨峰谷 慧王晓容代 丽谢寅峰*
南方现代林业协同创新中心,南京林业大学生物与环境学院,江苏 南京 210037
MA Yingli SHI Lei ZHANG Yufeng GU Hui WANG Xiaorong DAI Li XIE Yinfeng*
Co-Innovation Center for the Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing 210037, China
太子参 萘乙酸 块根 皂甙 微量元素 氨基酸
Keywords:Pseudostellaria heterophylla NAA root tuber saponin trace elements amino acids
【目的】探究萘乙酸(NAA)对太子参生长及其块根有效成分含量的影响,以期提高太子参产量并改善品质,为太子参高效栽培提供理论和应用依据。【方法】通过不同质量浓度萘乙酸(CK、10、20、50 mg/L)处理后,测定太子参生长指标、有效成分皂甙、微量元素、氨基酸含量变化,并进行数据分析。【结果】适宜质量浓度的萘乙酸处理可以显著提高太子参块根产量和有效成分的含量,以10 mg/L质量浓度处理组效果最佳,块根干、鲜产量、皂甙产量、氨基酸总量和药用氨基酸含量分别比对照增加39.88%、39.36%、60.69%、39.77%、43.42%,且差异显著(P<0.05)。在人体必需的微量元素中,除Cu元素外,Fe、Mn、Zn、Co和Se 5种元素均以10 mg/L NAA溶液处理组含量最高,而人体常量元素Ca、Mg在NAA质量浓度为50 mg/L时含量最高。【结论】适度质量浓度萘乙酸不仅可以提高太子参块根产量,还可以提高其主要药用成分的含量,改善其品质。太子参块根中皂甙含量与Fe、Co和Se含量及苏氨酸等氨基酸含量呈极显著正相关性。
Abstract: 【Objective】 To improve the yield and quality of Pseudostellaria heterophylla and provide a theoretical and practical basis for high-yield cultivation of P. heterophylla, the effects of NAA(naphthalene acetic acid)on the growth and active ingredients in the root tuber of P. heterophylla seedlings were explored.【Methods】After treatment with different concentrations NAA(CK, 10, 20, 50 mg/L), growth indexes and the contents of active ingredients of P. heterophylla were determined. The contents of saponin, trace elements and amino acids were measured by HPLC, ICP-OES and a high speed amino acid analyzer, respectively. 【Results】The results showed that the appropriate concentrations of NAA could significantly increase root tuber yield and active ingredient contents of P. heterophylla. The treatment with 10 mg/L concentration had the best effect. The dry and fresh weight of root tuber yield per unit area, and saponin yield, significantly increased by 39.88%, 39.36% and 60.69%, respectively, compared with that of the control. The contents of the mineral elements Fe, Mn, Zn, Co and Se were the highest with the concentration of 10 mg/L NAA, except for Cu, and were significantly higher than that of the control. However, the maximal increase in human constant elements Ca and Mg was attained with the 50 mg/L treatment. NAA treatments could increase contents of various amino acids in the root tubers of P. heterophylla by different degrees. The contents of total amino acids and medicinal amino acids were the highest for the 10 mg/L NAA treatment and significantly increased by 39.77%, 43.42%, respectively, compared with that of the control. 【Conclusion】The application of the appropriate concentrations of NAA were effective in increasing root tuber yield and active ingredient contents of P. heterophylla, improving its quality. Correlation analysis revealed relationships between saponin content and the contents of Fe, Co, Se, threonine, and other amino acids were significantly positive.


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基金项目:江苏高校优势学科建设工程资助项目(PAPD); 南京林业大学优秀博士学位论文创新基金项目 第一作者:马迎莉(yli_ma@163.com),博士生。*通信作者:谢寅峰(xxyy@njfu.edu.cn),教授。
更新日期/Last Update: 2018-06-06